ID   PIFI_ARATH              Reviewed;         268 AA.
AC   Q9LVZ5; A0A178V9N9; Q9FPH6;
DT   17-JUN-2020, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   25-MAY-2022, entry version 133.
DE   RecName: Full=Protein POST-ILLUMINATION CHLOROPHYLL FLUORESCENCE INCREASE, chloroplastic {ECO:0000303|PubMed:17573537};
DE   Flags: Precursor;
GN   Name=PIFI {ECO:0000303|PubMed:17573537};
GN   OrderedLocusNames=At3g15840 {ECO:0000312|Araport:AT3G15840};
GN   ORFNames=MSJ11.24 {ECO:0000312|EMBL:BAB02315.1};
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA   Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT   features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT   clones.";
RL   DNA Res. 7:131-135(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [4]
RP   FUNCTION, DISRUPTION PHENOTYPE, AND SUBCELLULAR LOCATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=17573537; DOI=10.1104/pp.107.103218;
RA   Wang D., Portis A.R. Jr.;
RT   "A novel nucleus-encoded chloroplast protein, PIFI, is involved in NAD(P)H
RT   dehydrogenase complex-mediated chlororespiratory electron transport in
RT   Arabidopsis.";
RL   Plant Physiol. 144:1742-1752(2007).
CC   -!- FUNCTION: Required during nonphotochemical quenching (PubMed:17573537).
CC       Involved in NAD(P)H dehydrogenase complex-mediated chlororespiratory
CC       electron transport which mediates electron flow from stromal reductants
CC       to the plastoquinone (PQ) pool during chlorophyll fluorescence increase
CC       after turning off actinic light, in response to photoinhibition
CC       conditions (e.g. high light intensities), and upon long-term mild heat
CC       stress (PubMed:17573537). {ECO:0000269|PubMed:17573537}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast stroma
CC       {ECO:0000269|PubMed:17573537}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=1;
CC         Comment=Additional isoforms seem to exist.
CC         {ECO:0000312|Araport:AT3G15840};
CC       Name=1;
CC         IsoId=Q9LVZ5-1; Sequence=Displayed;
CC   -!- DISRUPTION PHENOTYPE: Normal growth and photosynthesis in normal
CC       conditions (PubMed:17573537). Intact NDH complex, but altered NDH-
CC       dependent chlorophyll fluorescence increase after turning off actinic
CC       light and lower capacity for nonphotochemical quenching in high light
CC       intensities, due to altered nonphotochemical reduction of the
CC       plastoquinone (PQ) (PubMed:17573537). Increased sensitivity to
CC       photoinhibition and long-term mild heat stress (PubMed:17573537).
CC       {ECO:0000269|PubMed:17573537}.
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DR   EMBL; AB017071; BAB02315.1; -; Genomic_DNA.
DR   EMBL; CP002686; AEE75732.1; -; Genomic_DNA.
DR   EMBL; AF325024; AAG40376.1; -; mRNA.
DR   EMBL; AY035025; AAK59530.1; -; mRNA.
DR   EMBL; AY133879; AAM91813.1; -; mRNA.
DR   RefSeq; NP_566528.1; NM_112454.3. [Q9LVZ5-1]
DR   AlphaFoldDB; Q9LVZ5; -.
DR   SMR; Q9LVZ5; -.
DR   IntAct; Q9LVZ5; 2.
DR   STRING; 3702.AT3G15840.1; -.
DR   PaxDb; Q9LVZ5; -.
DR   PRIDE; Q9LVZ5; -.
DR   ProteomicsDB; 181653; -. [Q9LVZ5-1]
DR   EnsemblPlants; AT3G15840.1; AT3G15840.1; AT3G15840. [Q9LVZ5-1]
DR   GeneID; 820827; -.
DR   Gramene; AT3G15840.1; AT3G15840.1; AT3G15840. [Q9LVZ5-1]
DR   KEGG; ath:AT3G15840; -.
DR   Araport; AT3G15840; -.
DR   TAIR; locus:2093287; AT3G15840.
DR   eggNOG; ENOG502QUCM; Eukaryota.
DR   OrthoDB; 1120274at2759; -.
DR   PhylomeDB; Q9LVZ5; -.
DR   PRO; PR:Q9LVZ5; -.
DR   Proteomes; UP000006548; Chromosome 3.
DR   ExpressionAtlas; Q9LVZ5; baseline and differential.
DR   GO; GO:0009507; C:chloroplast; HDA:TAIR.
DR   GO; GO:0009570; C:chloroplast stroma; IDA:TAIR.
DR   GO; GO:0005829; C:cytosol; HDA:TAIR.
DR   GO; GO:0009579; C:thylakoid; HDA:TAIR.
DR   GO; GO:0070370; P:cellular heat acclimation; IMP:UniProtKB.
DR   GO; GO:0010478; P:chlororespiration; IMP:TAIR.
DR   GO; GO:0010196; P:nonphotochemical quenching; IMP:UniProtKB.
DR   InterPro; IPR044960; RCA-like.
DR   PANTHER; PTHR32429; PTHR32429; 1.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Chloroplast; Plastid; Reference proteome;
KW   Stress response; Transit peptide.
FT   TRANSIT         1..49
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000255"
FT   CHAIN           50..268
FT                   /note="Protein POST-ILLUMINATION CHLOROPHYLL FLUORESCENCE
FT                   INCREASE, chloroplastic"
FT                   /id="PRO_0000450266"
FT   CONFLICT        181
FT                   /note="W -> R (in Ref. 3; AAG40376)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        219
FT                   /note="T -> K (in Ref. 3; AAG40376)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   268 AA;  29691 MW;  8C0FC797F2F9A6A3 CRC64;
     MAAAANTSAV FASPSQPLSS KRSFLYSSRI GPILRRFPRK KLDLQVKAVA TTLAPLEEIK
     EYKLPSWAMF EMGTAPVYWK TMNGLPPTSG EKLKLFYNPA ASKLTLNEDY GVAFNGGFNQ
     PIMCGGEPRA MLKKDRGKAD SPIYTMQICI PKHAVNLIFS FTNGVDWDGP YRLQFQVPKR
     WQNKPIEFFN EGLANELSQD GACERAIFPD SNVVPTRCTM IANLTVEGGD RCNLDLVPGC
     MDTNSEHFNP YANVDDGSCP LELSDSDE