ID   PIFO_BOVIN              Reviewed;         192 AA.
AC   Q2TBS4;
DT   17-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT   24-JAN-2006, sequence version 1.
DT   25-MAY-2022, entry version 65.
DE   RecName: Full=Protein pitchfork;
GN   Name=PIFO;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Crossbred X Angus; TISSUE=Liver;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: During primary cilia disassembly, involved in cilia
CC       disassembly. Required specifically to control cilia retraction as well
CC       as the liberation and duplication of the basal body/centrosome. May act
CC       by stimulating AURKA activity at the basal body in a cell cycle-
CC       dependent manner (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with proteins involved in ciliary transport,
CC       including ARL13B, CETN1, KIF3A, RAB6A, RAB8A, TUBB1 and TUBG1.
CC       Interacts with AURKA (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle. Golgi apparatus, trans-Golgi
CC       network. Cytoplasm. Note=Accumulates specifically at the basal body and
CC       ciliary necklace during the early steps of cilia assembly and
CC       disassembly, when structural, functional and regulatory proteins are
CC       delivered to cilia. At S phase, accumulates in vesicles and declines
CC       during mitosis (By similarity). {ECO:0000250}.
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DR   EMBL; BC109727; AAI09728.1; -; mRNA.
DR   RefSeq; NP_001069932.1; NM_001076464.2.
DR   AlphaFoldDB; Q2TBS4; -.
DR   STRING; 9913.ENSBTAP00000010125; -.
DR   PaxDb; Q2TBS4; -.
DR   GeneID; 617486; -.
DR   KEGG; bta:617486; -.
DR   eggNOG; ENOG502RZWF; Eukaryota.
DR   InParanoid; Q2TBS4; -.
DR   OrthoDB; 1094638at2759; -.
DR   Proteomes; UP000009136; Unplaced.
DR   GO; GO:0036064; C:ciliary basal body; ISS:UniProtKB.
DR   GO; GO:0031410; C:cytoplasmic vesicle; IEA:UniProtKB-KW.
DR   GO; GO:0005802; C:trans-Golgi network; ISS:UniProtKB.
DR   GO; GO:0030030; P:cell projection organization; IEA:UniProtKB-KW.
DR   GO; GO:0033674; P:positive regulation of kinase activity; ISS:UniProtKB.
DR   GO; GO:0031344; P:regulation of cell projection organization; ISS:UniProtKB.
DR   InterPro; IPR033602; Pitchfork.
DR   PANTHER; PTHR31508; PTHR31508; 1.
PE   2: Evidence at transcript level;
KW   Cilium biogenesis/degradation; Cytoplasm; Cytoplasmic vesicle;
KW   Golgi apparatus; Reference proteome.
FT   CHAIN           1..192
FT                   /note="Protein pitchfork"
FT                   /id="PRO_0000284525"
SQ   SEQUENCE   192 AA;  22173 MW;  234D421FFB2ED7F5 CRC64;
     MCFSQAETLV NYSFGTCQHR KIFPHFFPSD LLGNKFLPIR GVPHRGPGCY IAEESKDLAY
     SLAKIPTSIK GYGFGARTAV RFKPKNKDVT PYPGMYQTVN LQEQTHKPNF APFNTLSPRF
     RTYSKDPCYP GPGTYKLEKK EPQKVTWPMK FGSPDWSQVP CLQKRTLKTE LSTDKDFRKH
     RNRLAYLSLY YN