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PIG17_CLAP2
ID   PIG17_CLAP2             Reviewed;         292 AA.
AC   M1W848;
DT   29-SEP-2021, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2013, sequence version 1.
DT   03-AUG-2022, entry version 28.
DE   RecName: Full=Short chain dehydrogenase/reductase CPUR_05418 {ECO:0000303|PubMed:30996871};
DE            Short=SDR CPUR_05418 {ECO:0000303|PubMed:30996871};
DE            EC=1.1.1.- {ECO:0000269|PubMed:32105084};
DE   AltName: Full=Ergochrome biosynthesis cluster protein CPUR_05418 {ECO:0000303|PubMed:30996871};
GN   ORFNames=CPUR_05418;
OS   Claviceps purpurea (strain 20.1) (Ergot fungus) (Sphacelia segetum).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Hypocreomycetidae; Hypocreales; Clavicipitaceae; Claviceps.
OX   NCBI_TaxID=1111077;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=20.1;
RX   PubMed=23468653; DOI=10.1371/journal.pgen.1003323;
RA   Schardl C.L., Young C.A., Hesse U., Amyotte S.G., Andreeva K., Calie P.J.,
RA   Fleetwood D.J., Haws D.C., Moore N., Oeser B., Panaccione D.G.,
RA   Schweri K.K., Voisey C.R., Farman M.L., Jaromczyk J.W., Roe B.A.,
RA   O'Sullivan D.M., Scott B., Tudzynski P., An Z., Arnaoudova E.G.,
RA   Bullock C.T., Charlton N.D., Chen L., Cox M., Dinkins R.D., Florea S.,
RA   Glenn A.E., Gordon A., Gueldener U., Harris D.R., Hollin W., Jaromczyk J.,
RA   Johnson R.D., Khan A.K., Leistner E., Leuchtmann A., Li C., Liu J., Liu J.,
RA   Liu M., Mace W., Machado C., Nagabhyru P., Pan J., Schmid J., Sugawara K.,
RA   Steiner U., Takach J.E., Tanaka E., Webb J.S., Wilson E.V., Wiseman J.L.,
RA   Yoshida R., Zeng Z.;
RT   "Plant-symbiotic fungi as chemical engineers: Multi-genome analysis of the
RT   Clavicipitaceae reveals dynamics of alkaloid loci.";
RL   PLoS Genet. 9:E1003323-E1003323(2013).
RN   [2]
RP   FUNCTION.
RX   PubMed=28955461; DOI=10.1186/s40694-016-0020-z;
RA   Neubauer L., Dopstadt J., Humpf H.U., Tudzynski P.;
RT   "Identification and characterization of the ergochrome gene cluster in the
RT   plant pathogenic fungus Claviceps purpurea.";
RL   Fungal Biol. Biotechnol. 3:2-2(2016).
RN   [3]
RP   IDENTIFICATION, AND FUNCTION.
RX   PubMed=30996871; DOI=10.1039/c8sc05126g;
RA   Greco C., de Mattos-Shipley K., Bailey A.M., Mulholland N.P., Vincent J.L.,
RA   Willis C.L., Cox R.J., Simpson T.J.;
RT   "Structure revision of cryptosporioptides and determination of the genetic
RT   basis for dimeric xanthone biosynthesis in fungi.";
RL   Chem. Sci. 10:2930-2939(2019).
RN   [4]
RP   FUNCTION, CATALYTIC ACTIVITY, AND PATHWAY.
RX   PubMed=32105084; DOI=10.1021/acs.orglett.0c00285;
RA   Wei X., Matsuda Y.;
RT   "Unraveling the fungal strategy for tetrahydroxanthone biosynthesis and
RT   diversification.";
RL   Org. Lett. 22:1919-1923(2020).
CC   -!- FUNCTION: Short chain dehydrogenase/reductase; part of the ergochrome
CC       gene cluster responsible for the typical purple-black color of the
CC       ergot sclerotia (Probable). The ergochrome gene cluster produces
CC       several ergot pigments including the yellow ergochrome secalonic acid
CC       and its derivatives, as well as the red anthraquinones endocrocin and
CC       clavorubin (PubMed:28955461). The pathway begins with the synthesis of
CC       atrochrysone thioester by the polyketide synthase (PKS) CPUR_05437 (By
CC       similarity). The atrochrysone carboxyl ACP thioesterase CPUR_05436 then
CC       breaks the thioester bond and releases the atrochrysone carboxylic acid
CC       from CPUR_05437 (By similarity). The atrochrysone carboxylic acid is
CC       then converted to atrochrysone which is further transformed into emodin
CC       anthrone (By similarity). The next step is performed by the anthrone
CC       oxygenase CPUR_05434 that catalyzes the oxidation of emodinanthrone to
CC       emodin (By similarity). Emodin is further modified to yield
CC       monodictyphenone via several steps involving CPUR_05427, CPUR_05428,
CC       CPUR_05429 and CPUR_05430 (By similarity). The short chain
CC       dehydrogenase/reductase CPUR_05418 then catalyzes the C-5 ketoreduction
CC       to give the xanthone skeleton of the monomeric units (PubMed:32105084).
CC       Ergochromes formation requires further dimerization steps of different
CC       xanthone units, probably catalyzed by the cytochrome P450 monooxygenase
CC       CPUR_05419 (PubMed:28955461). CPUR_05425, CPUR_05426 and CPUR_05431 are
CC       unique to Claviceps, thus it is likely that they are involved in
CC       further modification of xanthone units or in their dimerization
CC       (PubMed:28955461). The yellow ergochromes and the red anthraquinone
CC       pigments endocrocin and clavorubin are products from the same PKS
CC       derived precursors and the latter are likely shunt products in the
CC       pathway of xanthone biosynthesis (PubMed:28955461). It is proposed that
CC       atrochrysone carboxylic acid released from the PKS CPUR_05437 can also
CC       be converted to endocrocin anthrone which is further oxidized into
CC       endocrocin by CPUR_05435 (By similarity). Endocrocin could be then
CC       modified to clavorubin, possibly by CPUR_05423 and CPUR_05431
CC       (PubMed:28955461). Clavorubin is the principal anthraquinone metabolite
CC       produced by the cluster with a much higher yield compared to endocrocin
CC       (PubMed:28955461). {ECO:0000250|UniProtKB:Q4W944,
CC       ECO:0000250|UniProtKB:Q5BH30, ECO:0000269|PubMed:28955461,
CC       ECO:0000269|PubMed:32105084, ECO:0000305|PubMed:30996871}.
CC   -!- PATHWAY: Secondary metabolite biosynthesis.
CC       {ECO:0000269|PubMed:32105084}.
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC       family. {ECO:0000305}.
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DR   EMBL; CAGA01000032; CCE31565.1; -; Genomic_DNA.
DR   SMR; M1W848; -.
DR   EnsemblFungi; CCE31565; CCE31565; CPUR_05418.
DR   VEuPathDB; FungiDB:CPUR_05418; -.
DR   eggNOG; KOG4169; Eukaryota.
DR   HOGENOM; CLU_010194_8_2_1; -.
DR   OrthoDB; 1194344at2759; -.
DR   Proteomes; UP000016801; Unassembled WGS sequence.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR002347; SDR_fam.
DR   Pfam; PF00106; adh_short; 1.
DR   PRINTS; PR00081; GDHRDH.
DR   SUPFAM; SSF51735; SSF51735; 1.
PE   1: Evidence at protein level;
KW   NAD; NADP; Oxidoreductase; Reference proteome.
FT   CHAIN           1..292
FT                   /note="Short chain dehydrogenase/reductase CPUR_05418"
FT                   /id="PRO_0000453454"
FT   ACT_SITE        186
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250|UniProtKB:Q5BEK1"
FT   BINDING         43..51
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250|UniProtKB:Q92506"
FT   BINDING         70..71
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250|UniProtKB:Q92506"
FT   BINDING         95..97
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250|UniProtKB:Q92506"
FT   BINDING         186..190
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250|UniProtKB:Q92506"
FT   BINDING         221..223
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250|UniProtKB:Q92506"
SQ   SEQUENCE   292 AA;  31092 MW;  7AC8583E37D7C69D CRC64;
     MASITTTTNF FESIPRPTKT YRSASYDRIS TRHGFNGTGK TVLITGGSSG IGLSIAKAFA
     GAGVARIAIL SRSRATQLTA KAEIEAAYPS TSILLFEASV TDADRFASVL EELAGVHVLV
     LCAAAVHARV PLAELSGKDV QHVFDTNSVS TVNLARLYTA TPGKDKTILH VSSAVAQMYA
     PLRSVYGASK AAAVQAMQHL AREHQGTGDR VRVFSFHPGA ITTPASGAIY TPGAVQWDDA
     DLPAHFSLWL AGPESDFLNG RYVWANWDVD ELVALKERLA CDGRFLTIGL VL
 
 
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