PIG20_CLAP2
ID PIG20_CLAP2 Reviewed; 618 AA.
AC M1WCE9;
DT 29-SEP-2021, integrated into UniProtKB/Swiss-Prot.
DT 29-SEP-2021, sequence version 2.
DT 23-FEB-2022, entry version 21.
DE RecName: Full=Transcriptional regulator CPUR_05421 {ECO:0000303|PubMed:30996871};
DE AltName: Full=Ergochrome biosynthesis cluster protein CPUR_05421 {ECO:0000303|PubMed:30996871};
GN ORFNames=CPUR_05421;
OS Claviceps purpurea (strain 20.1) (Ergot fungus) (Sphacelia segetum).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Hypocreomycetidae; Hypocreales; Clavicipitaceae; Claviceps.
OX NCBI_TaxID=1111077;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=20.1;
RX PubMed=23468653; DOI=10.1371/journal.pgen.1003323;
RA Schardl C.L., Young C.A., Hesse U., Amyotte S.G., Andreeva K., Calie P.J.,
RA Fleetwood D.J., Haws D.C., Moore N., Oeser B., Panaccione D.G.,
RA Schweri K.K., Voisey C.R., Farman M.L., Jaromczyk J.W., Roe B.A.,
RA O'Sullivan D.M., Scott B., Tudzynski P., An Z., Arnaoudova E.G.,
RA Bullock C.T., Charlton N.D., Chen L., Cox M., Dinkins R.D., Florea S.,
RA Glenn A.E., Gordon A., Gueldener U., Harris D.R., Hollin W., Jaromczyk J.,
RA Johnson R.D., Khan A.K., Leistner E., Leuchtmann A., Li C., Liu J., Liu J.,
RA Liu M., Mace W., Machado C., Nagabhyru P., Pan J., Schmid J., Sugawara K.,
RA Steiner U., Takach J.E., Tanaka E., Webb J.S., Wilson E.V., Wiseman J.L.,
RA Yoshida R., Zeng Z.;
RT "Plant-symbiotic fungi as chemical engineers: Multi-genome analysis of the
RT Clavicipitaceae reveals dynamics of alkaloid loci.";
RL PLoS Genet. 9:E1003323-E1003323(2013).
RN [2]
RP FUNCTION.
RX PubMed=28955461; DOI=10.1186/s40694-016-0020-z;
RA Neubauer L., Dopstadt J., Humpf H.U., Tudzynski P.;
RT "Identification and characterization of the ergochrome gene cluster in the
RT plant pathogenic fungus Claviceps purpurea.";
RL Fungal Biol. Biotechnol. 3:2-2(2016).
RN [3]
RP IDENTIFICATION, AND FUNCTION.
RX PubMed=30996871; DOI=10.1039/c8sc05126g;
RA Greco C., de Mattos-Shipley K., Bailey A.M., Mulholland N.P., Vincent J.L.,
RA Willis C.L., Cox R.J., Simpson T.J.;
RT "Structure revision of cryptosporioptides and determination of the genetic
RT basis for dimeric xanthone biosynthesis in fungi.";
RL Chem. Sci. 10:2930-2939(2019).
CC -!- FUNCTION: Transcriptional regulator; part of the ergochrome gene
CC cluster responsible for the typical purple-black color of the ergot
CC sclerotia (Probable). The ergochrome gene cluster produces several
CC ergot pigments including the yellow ergochrome secalonic acid and its
CC derivatives, as well as the red anthraquinones endocrocin and
CC clavorubin (PubMed:28955461). {ECO:0000269|PubMed:28955461,
CC ECO:0000305|PubMed:30996871}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00227}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CCE31568.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; CAGA01000032; CCE31568.1; ALT_SEQ; Genomic_DNA.
DR EnsemblFungi; CCE31568; CCE31568; CPUR_05421.
DR VEuPathDB; FungiDB:CPUR_05421; -.
DR eggNOG; ENOG502SKTR; Eukaryota.
DR HOGENOM; CLU_516777_0_0_1; -.
DR OrthoDB; 483063at2759; -.
DR PhylomeDB; M1WCE9; -.
DR Proteomes; UP000016801; Unassembled WGS sequence.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
PE 3: Inferred from homology;
KW DNA-binding; Metal-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..618
FT /note="Transcriptional regulator CPUR_05421"
FT /id="PRO_0000453510"
FT DNA_BIND 14..41
FT /note="Zn(2)-C6 fungal-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00227"
FT REGION 52..87
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 61..87
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 618 AA; 69659 MW; B6747697E9B166E3 CRC64;
MVAPHESQRV ADSCSHLVGR EIGCSRDLAG CRRCTSEGRA CSYSRSGVIH RTRRRNRANQ
DVTRSALYSS NTTPQTISDQ ATGRPCEGAD NTETAYDRLQ QIIPSRSHQS VRVIAELLEE
YTEAWQGSEA FEKLSKAPIA EFFVFSEPQT RCWLDNLNLI LEKEELLIFT IPPEVYAQLA
SSRPQEVQDR SWLVMFYSVA IYVEHSKAQN THRVATLSKL RRNLWLAFND VRLLIEPSPA
RIQALIILAT YVEEFMTPCV CWSLITKACT MLQALGIVHW RLDTATSDVR AMLFWRLNML
DKALALILCR APAFHREMSS QIAMPTLNRL MQCRPKRTKD YPPALFEAHY LHQMHLVSCI
MADVWHALYG QDTEKVQEVK ERLEAWYRQA KEVIEAAALV ERPLLTAKGA AAVDLGRQTL
RFQYLSLVVL LTVSSRQLRS QSIGPSQEML NLLSALGDAI PDHKGPYACL LWQYLHCPLA
AFGALWGELV MKTTANPEQS LKSMEAIAHL PLYLGKLGSR HALAARLQSI TARIVEQARM
IVNSQEAPEA LPVQDEPTNP GLPAPAAEIL PIVAPNESLD PTQMQPVDDF LDDRFIMQSD
PFFGTTFDWF AWGGNLEV