PIG21_CLAP2
ID PIG21_CLAP2 Reviewed; 577 AA.
AC M1WG91;
DT 29-SEP-2021, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2013, sequence version 1.
DT 03-AUG-2022, entry version 32.
DE RecName: Full=MFS-type transporter CPUR_05422 {ECO:0000303|PubMed:30996871};
DE AltName: Full=Ergochrome biosynthesis cluster protein CPUR_05422 {ECO:0000303|PubMed:30996871};
GN ORFNames=CPUR_05422;
OS Claviceps purpurea (strain 20.1) (Ergot fungus) (Sphacelia segetum).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Hypocreomycetidae; Hypocreales; Clavicipitaceae; Claviceps.
OX NCBI_TaxID=1111077;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=20.1;
RX PubMed=23468653; DOI=10.1371/journal.pgen.1003323;
RA Schardl C.L., Young C.A., Hesse U., Amyotte S.G., Andreeva K., Calie P.J.,
RA Fleetwood D.J., Haws D.C., Moore N., Oeser B., Panaccione D.G.,
RA Schweri K.K., Voisey C.R., Farman M.L., Jaromczyk J.W., Roe B.A.,
RA O'Sullivan D.M., Scott B., Tudzynski P., An Z., Arnaoudova E.G.,
RA Bullock C.T., Charlton N.D., Chen L., Cox M., Dinkins R.D., Florea S.,
RA Glenn A.E., Gordon A., Gueldener U., Harris D.R., Hollin W., Jaromczyk J.,
RA Johnson R.D., Khan A.K., Leistner E., Leuchtmann A., Li C., Liu J., Liu J.,
RA Liu M., Mace W., Machado C., Nagabhyru P., Pan J., Schmid J., Sugawara K.,
RA Steiner U., Takach J.E., Tanaka E., Webb J.S., Wilson E.V., Wiseman J.L.,
RA Yoshida R., Zeng Z.;
RT "Plant-symbiotic fungi as chemical engineers: Multi-genome analysis of the
RT Clavicipitaceae reveals dynamics of alkaloid loci.";
RL PLoS Genet. 9:E1003323-E1003323(2013).
RN [2]
RP FUNCTION.
RX PubMed=28955461; DOI=10.1186/s40694-016-0020-z;
RA Neubauer L., Dopstadt J., Humpf H.U., Tudzynski P.;
RT "Identification and characterization of the ergochrome gene cluster in the
RT plant pathogenic fungus Claviceps purpurea.";
RL Fungal Biol. Biotechnol. 3:2-2(2016).
RN [3]
RP IDENTIFICATION, AND FUNCTION.
RX PubMed=30996871; DOI=10.1039/c8sc05126g;
RA Greco C., de Mattos-Shipley K., Bailey A.M., Mulholland N.P., Vincent J.L.,
RA Willis C.L., Cox R.J., Simpson T.J.;
RT "Structure revision of cryptosporioptides and determination of the genetic
RT basis for dimeric xanthone biosynthesis in fungi.";
RL Chem. Sci. 10:2930-2939(2019).
CC -!- FUNCTION: MFS-type transporter; part of the ergochrome gene cluster
CC responsible for the typical purple-black color of the ergot sclerotia
CC (Probable). The ergochrome gene cluster produces several ergot pigments
CC including the yellow ergochrome secalonic acid and its derivatives, as
CC well as the red anthraquinones endocrocin and clavorubin
CC (PubMed:28955461). {ECO:0000269|PubMed:28955461,
CC ECO:0000305|PubMed:30996871}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. TCR/Tet
CC family. {ECO:0000305}.
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DR EMBL; CAGA01000032; CCE31569.1; -; Genomic_DNA.
DR SMR; M1WG91; -.
DR STRING; 1111077.M1WG91; -.
DR EnsemblFungi; CCE31569; CCE31569; CPUR_05422.
DR VEuPathDB; FungiDB:CPUR_05422; -.
DR eggNOG; KOG0254; Eukaryota.
DR HOGENOM; CLU_000960_22_1_1; -.
DR OrthoDB; 627633at2759; -.
DR PhylomeDB; M1WG91; -.
DR Proteomes; UP000016801; Unassembled WGS sequence.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
PE 3: Inferred from homology;
KW Membrane; Reference proteome; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..577
FT /note="MFS-type transporter CPUR_05422"
FT /id="PRO_0000453502"
FT TRANSMEM 52..72
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 93..113
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 123..143
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 157..177
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 184..204
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 212..232
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 249..269
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 285..305
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 326..346
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 359..379
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 383..403
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 416..436
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 449..469
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 525..545
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..49
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 554..577
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 15..30
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 577 AA; 61193 MW; B0465A528A4A0DDF CRC64;
MSAMSAMGKP EHGSATTSDL EHRATESSLE KQDVEAAPPG PVKPVDPSPK QSTLKTTLLL
ASVFLAMFLV AVDRTIISTA IPSITNDYNS LNDVGWYGSI YLLTCCAFQL LFGKLYAMYS
ARVVLLVSLV IFLAASALCG AAPNSEAFIV GRAISGVGSA GIFAGNIVAI VHTIPLAKRP
KIQGLMGAVM GLATIIGPLI GGAFTTRVTW RWCFYINLPF GGLALVAIFF YFKVPNRSPA
ADLSLKQKIL SLDIPGTILL VPAIVCLLLA LQWGGQVYDW NNKYIIVLLT LMAVLFVAFI
AVQICLPKTA TVPPRLLMHR SVVAGFLTTI AIGSAQYIFV YYIPIWFQTV RGVNAVDSGI
QLLPLMISFV VASIVGGLLN QRIGYYTALG IFGSSVMAVG AGLMTTWNLE ISQGKVIGYQ
ILFGFGMGLA FQTPNLAVQT VLPRPEVPMG IALMFFGQLL SAAIFVAVGQ NILANQLLSR
LAGIDGFEHL KSLILSGGVT AVIDAVPEES KHRVLVAYSD ALQQVFIVGL AMSCVGVVGT
SCMEWKNILK KPAPPAGPPA GAPTESAPVE TKAAGHT
