PIG3_CLAP2
ID PIG3_CLAP2 Reviewed; 153 AA.
AC M1VWN3;
DT 25-APR-2018, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2013, sequence version 1.
DT 25-MAY-2022, entry version 20.
DE RecName: Full=Ergochrome gene cluster protein CPUR_05425 {ECO:0000303|PubMed:28955461};
GN ORFNames=CPUR_05425;
OS Claviceps purpurea (strain 20.1) (Ergot fungus) (Sphacelia segetum).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Hypocreomycetidae; Hypocreales; Clavicipitaceae; Claviceps.
OX NCBI_TaxID=1111077;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=20.1;
RX PubMed=23468653; DOI=10.1371/journal.pgen.1003323;
RA Schardl C.L., Young C.A., Hesse U., Amyotte S.G., Andreeva K., Calie P.J.,
RA Fleetwood D.J., Haws D.C., Moore N., Oeser B., Panaccione D.G.,
RA Schweri K.K., Voisey C.R., Farman M.L., Jaromczyk J.W., Roe B.A.,
RA O'Sullivan D.M., Scott B., Tudzynski P., An Z., Arnaoudova E.G.,
RA Bullock C.T., Charlton N.D., Chen L., Cox M., Dinkins R.D., Florea S.,
RA Glenn A.E., Gordon A., Gueldener U., Harris D.R., Hollin W., Jaromczyk J.,
RA Johnson R.D., Khan A.K., Leistner E., Leuchtmann A., Li C., Liu J., Liu J.,
RA Liu M., Mace W., Machado C., Nagabhyru P., Pan J., Schmid J., Sugawara K.,
RA Steiner U., Takach J.E., Tanaka E., Webb J.S., Wilson E.V., Wiseman J.L.,
RA Yoshida R., Zeng Z.;
RT "Plant-symbiotic fungi as chemical engineers: Multi-genome analysis of the
RT Clavicipitaceae reveals dynamics of alkaloid loci.";
RL PLoS Genet. 9:E1003323-E1003323(2013).
RN [2]
RP FUNCTION, AND INDUCTION.
RX PubMed=28955461; DOI=10.1186/s40694-016-0020-z;
RA Neubauer L., Dopstadt J., Humpf H.U., Tudzynski P.;
RT "Identification and characterization of the ergochrome gene cluster in the
RT plant pathogenic fungus Claviceps purpurea.";
RL Fungal Biol. Biotechnol. 3:2-2(2016).
RN [3]
RP FUNCTION.
RX PubMed=32105084; DOI=10.1021/acs.orglett.0c00285;
RA Wei X., Matsuda Y.;
RT "Unraveling the fungal strategy for tetrahydroxanthone biosynthesis and
RT diversification.";
RL Org. Lett. 22:1919-1923(2020).
CC -!- FUNCTION: Part of the ergochrome gene cluster responsible for the
CC typical purple-black color of the ergot sclerotia (PubMed:28955461).
CC The ergochrome gene cluster produces several ergot pigments including
CC the yellow ergochrome secalonic acid and its derivatives, as well as
CC the red anthraquinones endocrocin and clavorubin (PubMed:28955461). The
CC pathway begins with the synthesis of atrochrysone thioester by the
CC polyketide synthase (PKS) CPUR_05437 (By similarity). The atrochrysone
CC carboxyl ACP thioesterase CPUR_05436 then breaks the thioester bond and
CC releases the atrochrysone carboxylic acid from CPUR_05437 (By
CC similarity). The atrochrysone carboxylic acid is then converted to
CC atrochrysone which is further transformed into emodin anthrone (By
CC similarity). The next step is performed by the anthrone oxygenase
CC CPUR_05434 that catalyzes the oxidation of emodinanthrone to emodin (By
CC similarity). Emodin is further modified to yield monodictyphenone via
CC several steps involving CPUR_05427, CPUR_05428, CPUR_05429 and
CC CPUR_05430 (By similarity). The short chain dehydrogenase/reductase
CC CPUR_05418 then catalyzes the C-5 ketoreduction to give the xanthone
CC skeleton of the monomeric units (PubMed:32105084). Ergochromes
CC formation requires further dimerization steps of different xanthone
CC units, probably catalyzed by the cytochrome P450 monooxygenase
CC CPUR_05419 (PubMed:28955461). CPUR_05425, CPUR_05426 and CPUR_05431 are
CC unique to Claviceps, thus it is likely that they are involved in
CC further modification of xanthone units or in their dimerization
CC (PubMed:28955461). The yellow ergochromes and the red anthraquinone
CC pigments endocrocin and clavorubin are products from the same PKS
CC derived precursors and the latter are likely shunt products in the
CC pathway of xanthone biosynthesis (PubMed:28955461). It is proposed that
CC atrochrysone carboxylic acid released from the PKS CPUR_05437 can also
CC be converted to endocrocin anthrone which is further oxidized into
CC endocrocin by CPUR_05435 (By similarity). Endocrocin could be then
CC modified to clavorubin, possibly by CPUR_05423 and CPUR_05431
CC (PubMed:28955461). Clavorubin is the principal anthraquinone metabolite
CC produced by the cluster with a much higher yield compared to endocrocin
CC (PubMed:28955461). {ECO:0000250|UniProtKB:Q4W944,
CC ECO:0000250|UniProtKB:Q5BH30, ECO:0000269|PubMed:28955461,
CC ECO:0000269|PubMed:32105084}.
CC -!- PATHWAY: Pigment biosynthesis. {ECO:0000305|PubMed:28955461}.
CC -!- INDUCTION: Expression correlates with the formation of the sclerotia
CC and thus the pigment production and is directly regulated by the
CC cluster-specific activator CPUR_05433 (PubMed:28955461).
CC {ECO:0000269|PubMed:28955461}.
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DR EMBL; CAGA01000032; CCE31572.1; -; Genomic_DNA.
DR AlphaFoldDB; M1VWN3; -.
DR SMR; M1VWN3; -.
DR EnsemblFungi; CCE31572; CCE31572; CPUR_05425.
DR VEuPathDB; FungiDB:CPUR_05425; -.
DR eggNOG; ENOG502SPRW; Eukaryota.
DR HOGENOM; CLU_108113_0_0_1; -.
DR OrthoDB; 1409451at2759; -.
DR PhylomeDB; M1VWN3; -.
DR Proteomes; UP000016801; Unassembled WGS sequence.
DR InterPro; IPR032710; NTF2-like_dom_sf.
DR SUPFAM; SSF54427; SSF54427; 1.
PE 2: Evidence at transcript level;
KW Reference proteome.
FT CHAIN 1..153
FT /note="Ergochrome gene cluster protein CPUR_05425"
FT /id="PRO_0000443974"
SQ SEQUENCE 153 AA; 17061 MW; BA6DF0689A2B7FE4 CRC64;
MPAPAEVQQA TLSKFVDAWK RWNADDFIGL WSDSFTFKVL PLSDGKPTRP RDKVAPLYRN
LIGTLTDYKL DVKHIVHDAS KGKACIYAVA SANAPCGDYK NEQAIFITFS ESGDKIESME
EMNDNAFRKE WDPKYHAYHG YGQPPTAKAA AGS
