PIG6_CLAP2
ID PIG6_CLAP2 Reviewed; 158 AA.
AC M1W851;
DT 25-APR-2018, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2013, sequence version 1.
DT 03-AUG-2022, entry version 24.
DE RecName: Full=Scytalone dehydratase-like protein CPUR_05428 {ECO:0000250|UniProtKB:C8VQ71};
DE EC=4.2.1.- {ECO:0000250|UniProtKB:C8VQ71};
DE AltName: Full=Ergochrome gene cluster protein CPUR_05428 {ECO:0000303|PubMed:28955461};
GN ORFNames=CPUR_05428;
OS Claviceps purpurea (strain 20.1) (Ergot fungus) (Sphacelia segetum).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Hypocreomycetidae; Hypocreales; Clavicipitaceae; Claviceps.
OX NCBI_TaxID=1111077;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=20.1;
RX PubMed=23468653; DOI=10.1371/journal.pgen.1003323;
RA Schardl C.L., Young C.A., Hesse U., Amyotte S.G., Andreeva K., Calie P.J.,
RA Fleetwood D.J., Haws D.C., Moore N., Oeser B., Panaccione D.G.,
RA Schweri K.K., Voisey C.R., Farman M.L., Jaromczyk J.W., Roe B.A.,
RA O'Sullivan D.M., Scott B., Tudzynski P., An Z., Arnaoudova E.G.,
RA Bullock C.T., Charlton N.D., Chen L., Cox M., Dinkins R.D., Florea S.,
RA Glenn A.E., Gordon A., Gueldener U., Harris D.R., Hollin W., Jaromczyk J.,
RA Johnson R.D., Khan A.K., Leistner E., Leuchtmann A., Li C., Liu J., Liu J.,
RA Liu M., Mace W., Machado C., Nagabhyru P., Pan J., Schmid J., Sugawara K.,
RA Steiner U., Takach J.E., Tanaka E., Webb J.S., Wilson E.V., Wiseman J.L.,
RA Yoshida R., Zeng Z.;
RT "Plant-symbiotic fungi as chemical engineers: Multi-genome analysis of the
RT Clavicipitaceae reveals dynamics of alkaloid loci.";
RL PLoS Genet. 9:E1003323-E1003323(2013).
RN [2]
RP FUNCTION, AND INDUCTION.
RX PubMed=28955461; DOI=10.1186/s40694-016-0020-z;
RA Neubauer L., Dopstadt J., Humpf H.U., Tudzynski P.;
RT "Identification and characterization of the ergochrome gene cluster in the
RT plant pathogenic fungus Claviceps purpurea.";
RL Fungal Biol. Biotechnol. 3:2-2(2016).
RN [3]
RP FUNCTION.
RX PubMed=32105084; DOI=10.1021/acs.orglett.0c00285;
RA Wei X., Matsuda Y.;
RT "Unraveling the fungal strategy for tetrahydroxanthone biosynthesis and
RT diversification.";
RL Org. Lett. 22:1919-1923(2020).
CC -!- FUNCTION: Scytalone dehydratase-like protein; part of the ergochrome
CC gene cluster responsible for the typical purple-black color of the
CC ergot sclerotia (PubMed:28955461). The ergochrome gene cluster produces
CC several ergot pigments including the yellow ergochrome secalonic acid
CC and its derivatives, as well as the red anthraquinones endocrocin and
CC clavorubin (PubMed:28955461). The pathway begins with the synthesis of
CC atrochrysone thioester by the polyketide synthase (PKS) CPUR_05437 (By
CC similarity). The atrochrysone carboxyl ACP thioesterase CPUR_05436 then
CC breaks the thioester bond and releases the atrochrysone carboxylic acid
CC from CPUR_05437 (By similarity). The atrochrysone carboxylic acid is
CC then converted to atrochrysone which is further transformed into emodin
CC anthrone (By similarity). The next step is performed by the anthrone
CC oxygenase CPUR_05434 that catalyzes the oxidation of emodinanthrone to
CC emodin (By similarity). Emodin is further modified to yield
CC monodictyphenone via several steps involving CPUR_05427, CPUR_05428,
CC CPUR_05429 and CPUR_05430 (By similarity). The short chain
CC dehydrogenase/reductase CPUR_05418 then catalyzes the C-5 ketoreduction
CC to give the xanthone skeleton of the monomeric units (PubMed:32105084).
CC Ergochromes formation requires further dimerization steps of different
CC xanthone units, probably catalyzed by the cytochrome P450 monooxygenase
CC CPUR_05419 (PubMed:28955461). CPUR_05425, CPUR_05426 and CPUR_05431 are
CC unique to Claviceps, thus it is likely that they are involved in
CC further modification of xanthone units or in their dimerization
CC (PubMed:28955461). The yellow ergochromes and the red anthraquinone
CC pigments endocrocin and clavorubin are products from the same PKS
CC derived precursors and the latter are likely shunt products in the
CC pathway of xanthone biosynthesis (PubMed:28955461). It is proposed that
CC atrochrysone carboxylic acid released from the PKS CPUR_05437 can also
CC be converted to endocrocin anthrone which is further oxidized into
CC endocrocin by CPUR_05435 (By similarity). Endocrocin could be then
CC modified to clavorubin, possibly by CPUR_05423 and CPUR_05431
CC (PubMed:28955461). Clavorubin is the principal anthraquinone metabolite
CC produced by the cluster with a much higher yield compared to endocrocin
CC (PubMed:28955461). {ECO:0000250|UniProtKB:C8VQ71,
CC ECO:0000250|UniProtKB:Q4W944, ECO:0000269|PubMed:28955461,
CC ECO:0000269|PubMed:32105084}.
CC -!- PATHWAY: Pigment biosynthesis. {ECO:0000305|PubMed:28955461}.
CC -!- INDUCTION: Expression correlates with the formation of the sclerotia
CC and thus the pigment production and is directly regulated by the
CC cluster-specific activator CPUR_05433 (PubMed:28955461).
CC {ECO:0000269|PubMed:28955461}.
CC -!- SIMILARITY: Belongs to the scytalone dehydratase family. {ECO:0000305}.
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DR EMBL; CAGA01000032; CCE31575.1; -; Genomic_DNA.
DR AlphaFoldDB; M1W851; -.
DR SMR; M1W851; -.
DR STRING; 1111077.M1W851; -.
DR EnsemblFungi; CCE31575; CCE31575; CPUR_05428.
DR VEuPathDB; FungiDB:CPUR_05428; -.
DR eggNOG; ENOG502SNND; Eukaryota.
DR HOGENOM; CLU_101889_1_0_1; -.
DR OrthoDB; 1377897at2759; -.
DR PhylomeDB; M1W851; -.
DR Proteomes; UP000016801; Unassembled WGS sequence.
DR GO; GO:0030411; F:scytalone dehydratase activity; IEA:InterPro.
DR GO; GO:0006582; P:melanin metabolic process; IEA:InterPro.
DR InterPro; IPR032710; NTF2-like_dom_sf.
DR InterPro; IPR004235; Scytalone_dehydratase.
DR Pfam; PF02982; Scytalone_dh; 1.
DR PIRSF; PIRSF024851; SCD1; 1.
DR SUPFAM; SSF54427; SSF54427; 1.
PE 2: Evidence at transcript level;
KW Lyase; Reference proteome.
FT CHAIN 1..158
FT /note="Scytalone dehydratase-like protein CPUR_05428"
FT /id="PRO_0000443976"
FT ACT_SITE 79
FT /evidence="ECO:0000250|UniProtKB:P56221"
FT ACT_SITE 104
FT /evidence="ECO:0000250|UniProtKB:P56221"
FT BINDING 24
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:P56221"
FT BINDING 44
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:P56221"
SQ SEQUENCE 158 AA; 18042 MW; F16699272171125A CRC64;
MSKTPSYEDA TACQAALFEW AESYDLKDWN RLSKCIAPTL QIDYRAVFGQ FWEAMPADEF
VGMISSPGFL GNPLIKTQHF VGMTRWTQTS EDSVTGRHQM RVAHQKYADA QMKEVVLKGH
GHGGATTWFR KVDGDWKFAG IEPEIRWAEF DLDKIFAE
