ID   PIGB_BOVIN              Reviewed;         541 AA.
AC   Q1LZA0;
DT   25-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT   30-MAY-2006, sequence version 1.
DT   03-AUG-2022, entry version 78.
DE   RecName: Full=GPI mannosyltransferase 3;
DE            EC=2.4.1.-;
DE   AltName: Full=GPI mannosyltransferase III;
DE            Short=GPI-MT-III;
DE   AltName: Full=Phosphatidylinositol-glycan biosynthesis class B protein;
DE            Short=PIG-B;
GN   Name=PIGB;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Hereford; TISSUE=Ascending colon;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Mannosyltransferase involved in glycosylphosphatidylinositol-
CC       anchor biosynthesis. Transfers the third alpha-1,2-mannose to Man2-
CC       GlcN-acyl-PI during GPI precursor assembly (By similarity).
CC       {ECO:0000250}.
CC   -!- PATHWAY: Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor
CC       biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250};
CC       Multi-pass membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 22 family. PIGB
CC       subfamily. {ECO:0000305}.
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DR   EMBL; BC116127; AAI16128.1; -; mRNA.
DR   RefSeq; NP_001071523.1; NM_001078055.1.
DR   AlphaFoldDB; Q1LZA0; -.
DR   STRING; 9913.ENSBTAP00000016934; -.
DR   CAZy; GT22; Glycosyltransferase Family 22.
DR   PRIDE; Q1LZA0; -.
DR   Ensembl; ENSBTAT00000016934; ENSBTAP00000016934; ENSBTAG00000012740.
DR   GeneID; 616044; -.
DR   KEGG; bta:616044; -.
DR   CTD; 9488; -.
DR   VEuPathDB; HostDB:ENSBTAG00000012740; -.
DR   VGNC; VGNC:97300; PIGB.
DR   GeneTree; ENSGT00950000183090; -.
DR   InParanoid; Q1LZA0; -.
DR   OMA; HHMVFNN; -.
DR   OrthoDB; 901894at2759; -.
DR   UniPathway; UPA00196; -.
DR   Proteomes; UP000009136; Chromosome 10.
DR   Bgee; ENSBTAG00000012740; Expressed in neutrophil and 104 other tissues.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IBA:GO_Central.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0000026; F:alpha-1,2-mannosyltransferase activity; IBA:GO_Central.
DR   GO; GO:0004376; F:glycolipid mannosyltransferase activity; IEA:InterPro.
DR   GO; GO:0000030; F:mannosyltransferase activity; IBA:GO_Central.
DR   GO; GO:0006506; P:GPI anchor biosynthetic process; IBA:GO_Central.
DR   InterPro; IPR005599; GPI_mannosylTrfase.
DR   InterPro; IPR039521; PIG-B/GPI10.
DR   PANTHER; PTHR22760; PTHR22760; 1.
DR   PANTHER; PTHR22760:SF4; PTHR22760:SF4; 1.
DR   Pfam; PF03901; Glyco_transf_22; 1.
PE   2: Evidence at transcript level;
KW   Endoplasmic reticulum; Glycoprotein; Glycosyltransferase;
KW   GPI-anchor biosynthesis; Membrane; Reference proteome; Transferase;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..541
FT                   /note="GPI mannosyltransferase 3"
FT                   /id="PRO_0000246250"
FT   TRANSMEM        53..73
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        126..146
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        182..202
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        214..234
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        245..265
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        305..325
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        330..350
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        352..372
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        377..397
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        18
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        417
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   541 AA;  63591 MW;  AE94AC8E1A6CB200 CRC64;
     MERDDGGGGG LFFRGLQNRS HEKTKLRTRK STLYLSPQKS TGRCGDLGEN IYLVLFIIAL
     RICNCFLVQT SFVPDEYWQS LEVAHRMVFN YGYLTWEWTE RLRGYTYPLI FASIYKILHL
     LGKDSVHLLI WIPRLAQALL SAIADLRLYS LMKQLENQQV ARWVFFCQLC SWFTWYCCTR
     TLTNTMETVL TIIALFYYPL EGSKSMNSVK YSSLVALAFI IRPTAVIPWI PLLFRHFWQE
     QRKLDLILHQ FLPVGFVTLS LSLIIDRIFF GQWTLVQYNF LKFNVLQDLG SFYGSHPWHW
     YFSQGFPAVL GTHLPFFIHG CFLAPKRYRI FLVTVLWTLL VYSMLSHKEF RFIYPVLPFC
     MVFCGYSLNN LKTWKKPALS FLFLSNMLLA LYTGLVHQRG TLDVMTNIQE LSYNNTNVSS
     ASVLMMMPCH STPYYSHVHY PLPMRFLQCP PDLTGKTDYL VEADMFYLNP LKWLYMEFQN
     DSQLPTHLIM FSVLEEEISP FLISNNYERT AVFFHTHFPE SRTGSHIYVY ERKLKGKLNQ
     R