ID   PIGB_DANRE              Reviewed;         536 AA.
AC   Q7SXZ1;
DT   25-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2003, sequence version 1.
DT   03-AUG-2022, entry version 91.
DE   RecName: Full=GPI mannosyltransferase 3;
DE            EC=2.4.1.-;
DE   AltName: Full=GPI mannosyltransferase III;
DE            Short=GPI-MT-III;
DE   AltName: Full=Phosphatidylinositol-glycan biosynthesis class B protein;
DE            Short=PIG-B;
GN   Name=pigb; ORFNames=zgc:63658;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=SJD;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Mannosyltransferase involved in glycosylphosphatidylinositol-
CC       anchor biosynthesis. Transfers the third alpha-1,2-mannose to Man2-
CC       GlcN-acyl-PI during GPI precursor assembly (By similarity).
CC       {ECO:0000250}.
CC   -!- PATHWAY: Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor
CC       biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250};
CC       Multi-pass membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 22 family. PIGB
CC       subfamily. {ECO:0000305}.
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DR   EMBL; BC055191; AAH55191.1; -; mRNA.
DR   RefSeq; NP_956770.1; NM_200476.1.
DR   AlphaFoldDB; Q7SXZ1; -.
DR   STRING; 7955.ENSDARP00000032121; -.
DR   CAZy; GT22; Glycosyltransferase Family 22.
DR   PaxDb; Q7SXZ1; -.
DR   GeneID; 393448; -.
DR   KEGG; dre:393448; -.
DR   CTD; 9488; -.
DR   ZFIN; ZDB-GENE-040426-1216; pigb.
DR   eggNOG; KOG1771; Eukaryota.
DR   InParanoid; Q7SXZ1; -.
DR   OrthoDB; 901894at2759; -.
DR   PhylomeDB; Q7SXZ1; -.
DR   Reactome; R-DRE-162710; Synthesis of glycosylphosphatidylinositol (GPI).
DR   UniPathway; UPA00196; -.
DR   PRO; PR:Q7SXZ1; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Unplaced.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IBA:GO_Central.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0000026; F:alpha-1,2-mannosyltransferase activity; IBA:GO_Central.
DR   GO; GO:0004376; F:glycolipid mannosyltransferase activity; IEA:InterPro.
DR   GO; GO:0000030; F:mannosyltransferase activity; IBA:GO_Central.
DR   GO; GO:0006506; P:GPI anchor biosynthetic process; IBA:GO_Central.
DR   InterPro; IPR005599; GPI_mannosylTrfase.
DR   InterPro; IPR039521; PIG-B/GPI10.
DR   PANTHER; PTHR22760; PTHR22760; 1.
DR   PANTHER; PTHR22760:SF4; PTHR22760:SF4; 1.
DR   Pfam; PF03901; Glyco_transf_22; 1.
PE   2: Evidence at transcript level;
KW   Endoplasmic reticulum; Glycoprotein; Glycosyltransferase;
KW   GPI-anchor biosynthesis; Membrane; Reference proteome; Transferase;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..536
FT                   /note="GPI mannosyltransferase 3"
FT                   /id="PRO_0000246253"
FT   TRANSMEM        40..60
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        118..138
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        206..226
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        243..263
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        297..317
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        322..342
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        344..364
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        369..389
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        15
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        176
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        467
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   536 AA;  62240 MW;  B16C0394921DE814 CRC64;
     MEKIRERFKQ SKSSNESVRL RKRSSILYST DDRKLQTLKI FGINITVFIV LVRLLNCVLV
     QTSFVPDEFW QSLEISHRMV FGYGYETWEW KEGIRGYSYP LLFALMYKLL QLISYDTVYL
     LVFVPRVFQA LLAAYADVKL YKLILQWETP DVAKWTCFCQ LCSWFAWFCC SRTLTNTTET
     ALTTLALCFY PLPGSKTHSS WKYLTLVSLA VVIRPTALIV WFPLIFYHFC TDQDKLKLIT
     HRYFPIGVLA LGVSTLIDSF FYGKWIFVQW NFLKFNVLHN VGEFYGSHPW HWYWTQGLPV
     VIGPHLPLVL HGCLLSTRKH TILLLTIIWT TAVYSLLAHK EFRFIYPVLP FCMIFCGLSL
     AKLQAWRKAA AGALLLFNLC PALYTGLVHQ RGALDVMHVL QPLCVSSQSP NPPELLFLMP
     CHSTPLYSHL HCPLKLRFLE CPPDLTGRQD YMDEADVFFS DPLHWLNTSF PLRSTLPSHL
     VLFDSLQKEI SSFLEENSFA KQAEIFHTHF PEGRVGKNIL IYSRRSEMKI NEGFIL