PIGB_DROME
ID PIGB_DROME Reviewed; 561 AA.
AC Q9VZM5; Q7KV63;
DT 25-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 2.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=GPI mannosyltransferase 3;
DE EC=2.4.1.-;
DE AltName: Full=GPI mannosyltransferase III;
DE Short=GPI-MT-III;
DE AltName: Full=Phosphatidylinositol-glycan biosynthesis class B protein {ECO:0000312|FlyBase:FBgn0035464};
GN Name=PIG-B {ECO:0000312|FlyBase:FBgn0035464};
GN ORFNames=CG12006 {ECO:0000312|FlyBase:FBgn0035464};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
RC STRAIN=Berkeley; TISSUE=Embryo;
RA Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M.,
RA Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A.,
RA Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A.,
RA Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S.,
RA Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E.;
RL Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Mannosyltransferase involved in glycosylphosphatidylinositol-
CC anchor biosynthesis. Transfers the third alpha-1,2-mannose to Man2-
CC GlcN-acyl-PI during GPI precursor assembly (By similarity).
CC {ECO:0000250}.
CC -!- PATHWAY: Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor
CC biosynthesis.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250};
CC Multi-pass membrane protein {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=A;
CC IsoId=Q9VZM5-1; Sequence=Displayed;
CC Name=B;
CC IsoId=Q9VZM5-2; Sequence=VSP_019841;
CC -!- SIMILARITY: Belongs to the glycosyltransferase 22 family. PIGB
CC subfamily. {ECO:0000305}.
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DR EMBL; AE014296; AAF47795.2; -; Genomic_DNA.
DR EMBL; AE014296; AAS64961.1; -; Genomic_DNA.
DR EMBL; BT003240; AAO24997.1; -; mRNA.
DR RefSeq; NP_647832.1; NM_139575.3. [Q9VZM5-1]
DR RefSeq; NP_995991.1; NM_206269.3. [Q9VZM5-2]
DR AlphaFoldDB; Q9VZM5; -.
DR BioGRID; 63936; 1.
DR IntAct; Q9VZM5; 7.
DR STRING; 7227.FBpp0073008; -.
DR CAZy; GT22; Glycosyltransferase Family 22.
DR GlyGen; Q9VZM5; 2 sites.
DR PaxDb; Q9VZM5; -.
DR PRIDE; Q9VZM5; -.
DR DNASU; 38446; -.
DR EnsemblMetazoa; FBtr0073149; FBpp0073008; FBgn0035464. [Q9VZM5-1]
DR EnsemblMetazoa; FBtr0073150; FBpp0073009; FBgn0035464. [Q9VZM5-2]
DR GeneID; 38446; -.
DR KEGG; dme:Dmel_CG12006; -.
DR UCSC; CG12006-RA; d. melanogaster. [Q9VZM5-1]
DR CTD; 38446; -.
DR FlyBase; FBgn0035464; PIG-B.
DR VEuPathDB; VectorBase:FBgn0035464; -.
DR eggNOG; KOG1771; Eukaryota.
DR GeneTree; ENSGT00950000183090; -.
DR InParanoid; Q9VZM5; -.
DR OMA; HHMVFNN; -.
DR PhylomeDB; Q9VZM5; -.
DR Reactome; R-DME-162710; Synthesis of glycosylphosphatidylinositol (GPI).
DR UniPathway; UPA00196; -.
DR BioGRID-ORCS; 38446; 0 hits in 1 CRISPR screen.
DR ChiTaRS; CG12006; fly.
DR GenomeRNAi; 38446; -.
DR PRO; PR:Q9VZM5; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0035464; Expressed in wing disc and 36 other tissues.
DR Genevisible; Q9VZM5; DM.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; ISS:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0000026; F:alpha-1,2-mannosyltransferase activity; IBA:GO_Central.
DR GO; GO:0004376; F:glycolipid mannosyltransferase activity; IEA:InterPro.
DR GO; GO:0000030; F:mannosyltransferase activity; ISS:UniProtKB.
DR GO; GO:0006506; P:GPI anchor biosynthetic process; ISS:UniProtKB.
DR InterPro; IPR005599; GPI_mannosylTrfase.
DR InterPro; IPR039521; PIG-B/GPI10.
DR PANTHER; PTHR22760; PTHR22760; 1.
DR PANTHER; PTHR22760:SF4; PTHR22760:SF4; 1.
DR Pfam; PF03901; Glyco_transf_22; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Endoplasmic reticulum; Glycoprotein;
KW Glycosyltransferase; GPI-anchor biosynthesis; Membrane; Reference proteome;
KW Transferase; Transmembrane; Transmembrane helix.
FT CHAIN 1..561
FT /note="GPI mannosyltransferase 3"
FT /id="PRO_0000246255"
FT TRANSMEM 3..25
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 64..84
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 110..130
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 155..175
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 195..215
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 246..266
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 275..295
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 328..348
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 525..546
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 398
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 456
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 475..504
FT /note="Missing (in isoform B)"
FT /evidence="ECO:0000305"
FT /id="VSP_019841"
SQ SEQUENCE 561 AA; 65007 MW; 5A94054C6E9DB239 CRC64;
MKLIYVFLLI LAVRLASVFV VQTYYVPDEY WQSLEVAHKL TFGYGYLTWE WVQGIRSYVY
PLLIAGLYKI LALLQLDSAH LLVVLPRIVQ ALLSAYSDYR FFVWTGKRKW ALFLILVPWF
WFYTGSRTLA NTLEASLTTI ALSYFPWYGE STAYLWPAAI CCFLRPTAAV IWLPLSLYHL
RRSRQNVLEL ILKRFVLIGL LVAGLGIAID TYWHGQLIVT PYEFLKYNIF NNIGSFYGSH
PWHWYFSVGL PTVLGINTLP FIFGVMETVK KSEKYPVSKQ LLITIFLTLV VLSAVEHKEF
RFVSPLLPLC LYVITDALSR WSIRASSTML WTTALVILVG NVMPAWYLST VHQKGPIELM
PKLREIAREY RDEREHQANI LFLMPCHSTP YYSHIHQNVT MRFLTCEPNL EKKEQYKDEA
DRFFEDPVHW INSHIPMHPL TALPTHVVLF DPLAENISVF LRNYRLLHRI EHAEVTRLEG
SQALVDQWSE ALGAQSPNLA SLLQNRQSRT GRSILVYQRL KKGEENAFNR GPDSGQHEPD
VHDHPPLEDL VLANENENLF N
