PIGC_ARATH
ID PIGC_ARATH Reviewed; 303 AA.
AC O64761;
DT 02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=Phosphatidylinositol N-acetylglucosaminyltransferase subunit C;
DE EC=2.4.1.198;
DE AltName: Full=Phosphatidylinositol-glycan biosynthesis class C protein;
GN Name=PIGC {ECO:0000305}; Synonyms=SETH1 {ECO:0000303|PubMed:14671020};
GN OrderedLocusNames=At2g34980 {ECO:0000312|Araport:AT2G34980};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP FUNCTION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=14671020; DOI=10.1105/tpc.014407;
RA Lalanne E., Honys D., Johnson A., Borner G.H.H., Lilley K.S., Dupree P.,
RA Grossniklaus U., Twell D.;
RT "SETH1 and SETH2, two components of the glycosylphosphatidylinositol anchor
RT biosynthetic pathway, are required for pollen germination and tube growth
RT in Arabidopsis.";
RL Plant Cell 16:229-240(2004).
CC -!- FUNCTION: Part of the complex catalyzing the transfer of N-
CC acetylglucosamine from UDP-N-acetylglucosamine to phosphatidylinositol,
CC the first step of GPI biosynthesis (Probable). Required for pollen
CC germination and pollen tube growth (PubMed:14671020).
CC {ECO:0000269|PubMed:14671020, ECO:0000305}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + UDP-N-
CC acetyl-alpha-D-glucosamine = a 6-(N-acetyl-alpha-D-glucosaminyl)-1-
CC phosphatidyl-1D-myo-inositol + H(+) + UDP; Xref=Rhea:RHEA:14789,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57265, ChEBI:CHEBI:57705,
CC ChEBI:CHEBI:57880, ChEBI:CHEBI:58223; EC=2.4.1.198;
CC -!- PATHWAY: Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor
CC biosynthesis. {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000305};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Expressed in roots, stems, leaves, flowers and
CC pollen grains. {ECO:0000269|PubMed:14671020}.
CC -!- DISRUPTION PHENOTYPE: Defective in pollen germination and pollen tube
CC growth. {ECO:0000269|PubMed:14671020}.
CC -!- SIMILARITY: Belongs to the PIGC family. {ECO:0000305}.
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DR EMBL; AC004238; AAC12837.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC09045.1; -; Genomic_DNA.
DR PIR; T00479; T00479.
DR RefSeq; NP_181043.1; NM_129050.2.
DR AlphaFoldDB; O64761; -.
DR STRING; 3702.AT2G34980.1; -.
DR PaxDb; O64761; -.
DR PRIDE; O64761; -.
DR ProteomicsDB; 235109; -.
DR EnsemblPlants; AT2G34980.1; AT2G34980.1; AT2G34980.
DR GeneID; 818062; -.
DR Gramene; AT2G34980.1; AT2G34980.1; AT2G34980.
DR KEGG; ath:AT2G34980; -.
DR Araport; AT2G34980; -.
DR TAIR; locus:2044727; AT2G34980.
DR eggNOG; KOG3059; Eukaryota.
DR HOGENOM; CLU_024002_2_0_1; -.
DR InParanoid; O64761; -.
DR OMA; HAFVMVT; -.
DR OrthoDB; 1241278at2759; -.
DR PhylomeDB; O64761; -.
DR BioCyc; ARA:AT2G34980-MON; -.
DR UniPathway; UPA00196; -.
DR PRO; PR:O64761; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O64761; baseline and differential.
DR GO; GO:0000506; C:glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; TAS:TAIR.
DR GO; GO:0017176; F:phosphatidylinositol N-acetylglucosaminyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0006506; P:GPI anchor biosynthetic process; TAS:TAIR.
DR GO; GO:0009846; P:pollen germination; IMP:TAIR.
DR GO; GO:0009860; P:pollen tube growth; IMP:TAIR.
DR InterPro; IPR009450; Plno_GlcNAc_GPI2.
DR PANTHER; PTHR12982; PTHR12982; 1.
DR Pfam; PF06432; GPI2; 1.
DR PIRSF; PIRSF016104; GPI2; 1.
PE 2: Evidence at transcript level;
KW Endoplasmic reticulum; Glycosyltransferase; GPI-anchor biosynthesis;
KW Membrane; Reference proteome; Transferase; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..303
FT /note="Phosphatidylinositol N-acetylglucosaminyltransferase
FT subunit C"
FT /id="PRO_0000438103"
FT TRANSMEM 59..79
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 87..107
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 120..140
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 143..163
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 179..199
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 209..229
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 234..254
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 259..279
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 303 AA; 34200 MW; 88E6BF82F4940C92 CRC64;
MESNLAGDSI PGPKWRKVAY GGMQIGYDDN YTDESFLEEM VMNANVVRRD LLKVMKDSVS
ISQYLCIVAL VVLVWVHTLE SSLDENSLLL LDLSLLASGF LILLLTEEKM LSLSLLLRYL
VNISFFTTGL YILAPIYQTL TRSISSDSIW AVTVSLLLLH LFLHDYSGST IRAPGALKTP
NLTSCISVNA SIVASVFVAS RLPSRLHVFA VMLFSLQVFL FAPLVTYCIK KFNFGLHLLF
SFALMGLTLY SIYALHRLFF LVFLSLVLLV NVVCPYWLIR MQEYKFEING PWDEAKLCFD
ITD
