PIGF_MOUSE
ID PIGF_MOUSE Reviewed; 219 AA.
AC O09101;
DT 13-AUG-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1997, sequence version 1.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=Phosphatidylinositol-glycan biosynthesis class F protein;
DE Short=PIG-F;
GN Name=Pigf;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=8786134; DOI=10.1006/geno.1996.0296;
RA Ohishi K., Kurimoto Y., Inoue N., Endo Y., Takeda J., Kinoshita T.;
RT "Cloning and characterization of the murine GPI anchor synthesis gene Pigf,
RT a homologue of the human PIGF gene.";
RL Genomics 34:340-346(1996).
RN [2]
RP FUNCTION, SUBUNIT, AND SUBCELLULAR LOCATION.
RC TISSUE=Testis;
RX PubMed=10781593; DOI=10.1074/jbc.m001913200;
RA Hong Y., Maeda Y., Watanabe R., Inoue N., Ohishi K., Kinoshita T.;
RT "Requirement of PIG-F and PIG-O for transferring phosphoethanolamine to the
RT third mannose in glycosylphosphatidylinositol.";
RL J. Biol. Chem. 275:20911-20919(2000).
CC -!- FUNCTION: Involved in GPI-anchor biosynthesis through the transfer of
CC ethanolamine phosphate to the third mannose of GPI.
CC {ECO:0000269|PubMed:10781593}.
CC -!- PATHWAY: Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor
CC biosynthesis.
CC -!- SUBUNIT: Forms a complex with PIGG and PIGO. PIGF is required to
CC stabilize PIGG and PIGO. {ECO:0000269|PubMed:10781593}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000269|PubMed:10781593}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:10781593}.
CC -!- SIMILARITY: Belongs to the PIGF family. {ECO:0000305}.
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DR EMBL; D50264; BAA08818.1; -; mRNA.
DR CCDS; CCDS29011.1; -.
DR RefSeq; NP_032864.1; NM_008838.1.
DR RefSeq; XP_006523894.1; XM_006523831.2.
DR AlphaFoldDB; O09101; -.
DR BioGRID; 202156; 2.
DR STRING; 10090.ENSMUSP00000024957; -.
DR EPD; O09101; -.
DR PaxDb; O09101; -.
DR PRIDE; O09101; -.
DR Antibodypedia; 29978; 100 antibodies from 25 providers.
DR DNASU; 18701; -.
DR Ensembl; ENSMUST00000024957; ENSMUSP00000024957; ENSMUSG00000024145.
DR GeneID; 18701; -.
DR KEGG; mmu:18701; -.
DR UCSC; uc008dun.1; mouse.
DR CTD; 5281; -.
DR MGI; MGI:99462; Pigf.
DR VEuPathDB; HostDB:ENSMUSG00000024145; -.
DR eggNOG; KOG3144; Eukaryota.
DR GeneTree; ENSGT00390000016617; -.
DR HOGENOM; CLU_1261153_0_0_1; -.
DR InParanoid; O09101; -.
DR OMA; AHKISRF; -.
DR OrthoDB; 1182539at2759; -.
DR PhylomeDB; O09101; -.
DR TreeFam; TF323878; -.
DR Reactome; R-MMU-162710; Synthesis of glycosylphosphatidylinositol (GPI).
DR UniPathway; UPA00196; -.
DR BioGRID-ORCS; 18701; 7 hits in 75 CRISPR screens.
DR ChiTaRS; Pigf; mouse.
DR PRO; PR:O09101; -.
DR Proteomes; UP000000589; Chromosome 17.
DR RNAct; O09101; protein.
DR Bgee; ENSMUSG00000024145; Expressed in spermatid and 246 other tissues.
DR Genevisible; O09101; MM.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IDA:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0051377; F:mannose-ethanolamine phosphotransferase activity; IBA:GO_Central.
DR GO; GO:0016780; F:phosphotransferase activity, for other substituted phosphate groups; TAS:MGI.
DR GO; GO:0006506; P:GPI anchor biosynthetic process; IDA:UniProtKB.
DR InterPro; IPR009580; GPI_biosynthesis_protein_Pig-F.
DR Pfam; PF06699; PIG-F; 1.
PE 1: Evidence at protein level;
KW Endoplasmic reticulum; GPI-anchor biosynthesis; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..219
FT /note="Phosphatidylinositol-glycan biosynthesis class F
FT protein"
FT /id="PRO_0000191760"
FT TRANSMEM 11..31
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 42..62
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 86..106
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 113..133
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 155..175
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 189..209
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 219 AA; 24825 MW; BD78A77E69C5BC1E CRC64;
MKDTDIKRLL YTNLLCVFSI FLSIFIPSFF VDNFSVLEAH LTWLCICSAS VTTVNLLSYL
VVKPNVSSKR SSLSHKVTRA LKCCVCFLMS CFLLHIIFVL YGAPLIELVL ETFLFAVVLS
TFTTVPCLCL LGPNLKAWLR VFSRNGVTSI WENSLQITTI SSFTGAWLGA FPIPLDWERP
WQVWPISCTL GATFGYVAGL VISPLWIYWN RKQLTYKNN
