ID   PIGG_SERS3              Reviewed;          87 AA.
AC   Q5W265;
DT   11-MAY-2016, integrated into UniProtKB/Swiss-Prot.
DT   07-DEC-2004, sequence version 1.
DT   25-MAY-2022, entry version 50.
DE   RecName: Full=Probable acyl carrier protein PigG {ECO:0000303|PubMed:17002325};
DE   AltName: Full=Peptidyl carrier protein {ECO:0000303|PubMed:17002325};
DE            Short=PCP {ECO:0000303|PubMed:17002325};
GN   Name=pigG {ECO:0000303|PubMed:15528645};
OS   Serratia sp. (strain ATCC 39006).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Yersiniaceae; Serratia; unclassified Serratia.
OX   NCBI_TaxID=104623;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 39006 / SC 11482;
RX   PubMed=15528645; DOI=10.1099/mic.0.27222-0;
RA   Harris A.K., Williamson N.R., Slater H., Cox A., Abbasi S., Foulds I.,
RA   Simonsen H.T., Leeper F.J., Salmond G.P.;
RT   "The Serratia gene cluster encoding biosynthesis of the red antibiotic,
RT   prodigiosin, shows species- and strain-dependent genome context
RT   variation.";
RL   Microbiology 150:3547-3560(2004).
RN   [2]
RP   FUNCTION, DISRUPTION PHENOTYPE, AND PATHWAY.
RC   STRAIN=ATCC 39006 / SC 11482;
RX   PubMed=15853884; DOI=10.1111/j.1365-2958.2005.04602.x;
RA   Williamson N.R., Simonsen H.T., Ahmed R.A., Goldet G., Slater H.,
RA   Woodley L., Leeper F.J., Salmond G.P.;
RT   "Biosynthesis of the red antibiotic, prodigiosin, in Serratia:
RT   identification of a novel 2-methyl-3-n-amyl-pyrrole (MAP) assembly pathway,
RT   definition of the terminal condensing enzyme, and implications for
RT   undecylprodigiosin biosynthesis in Streptomyces.";
RL   Mol. Microbiol. 56:971-989(2005).
RN   [3]
RP   FUNCTION, AND PATHWAY.
RC   STRAIN=ATCC 39006 / SC 11482;
RX   PubMed=17002325; DOI=10.1021/ja063611l;
RA   Garneau-Tsodikova S., Dorrestein P.C., Kelleher N.L., Walsh C.T.;
RT   "Protein assembly line components in prodigiosin biosynthesis:
RT   characterization of PigA,G,H,I,J.";
RL   J. Am. Chem. Soc. 128:12600-12601(2006).
CC   -!- FUNCTION: Involved in the biosynthesis of 4-methoxy-2,2'-bipyrrole-5-
CC       carbaldehyde (MBC), one of the terminal products involved in the
CC       biosynthesis of the red antibiotic prodigiosin (Pig). Carrier of the L-
CC       prolyl group transferred from L-prolyl-AMP by PigI.
CC       {ECO:0000269|PubMed:15853884, ECO:0000269|PubMed:17002325}.
CC   -!- PATHWAY: Antibiotic biosynthesis; prodigiosin biosynthesis.
CC       {ECO:0000305|PubMed:15853884, ECO:0000305|PubMed:17002325}.
CC   -!- DISRUPTION PHENOTYPE: Cells lacking this gene show a white phenotype
CC       and produce 2-methyl-3-n-amyl-pyrrole (MAP).
CC       {ECO:0000269|PubMed:15853884}.
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DR   EMBL; AJ833001; CAH55635.1; -; Genomic_DNA.
DR   RefSeq; WP_021014645.1; NZ_CP025085.1.
DR   PDB; 5JDX; NMR; -; A=1-87.
DR   PDBsum; 5JDX; -.
DR   AlphaFoldDB; Q5W265; -.
DR   BMRB; Q5W265; -.
DR   SMR; Q5W265; -.
DR   STRING; 104623.Ser39006_01375; -.
DR   KEGG; ag:CAH55635; -.
DR   eggNOG; COG0236; Bacteria.
DR   OrthoDB; 1943389at2; -.
DR   UniPathway; UPA01072; -.
DR   GO; GO:0017000; P:antibiotic biosynthetic process; IMP:UniProtKB.
DR   Gene3D; 1.10.1200.10; -; 1.
DR   InterPro; IPR036736; ACP-like_sf.
DR   InterPro; IPR009081; PP-bd_ACP.
DR   SUPFAM; SSF47336; SSF47336; 1.
DR   PROSITE; PS50075; CARRIER; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Phosphopantetheine; Phosphoprotein.
FT   CHAIN           1..87
FT                   /note="Probable acyl carrier protein PigG"
FT                   /id="PRO_0000436244"
FT   DOMAIN          1..78
FT                   /note="Carrier"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT   MOD_RES         36
FT                   /note="O-(pantetheine 4'-phosphoryl)serine"
FT                   /evidence="ECO:0000250|UniProtKB:P0A2W5,
FT                   ECO:0000255|PROSITE-ProRule:PRU00258"
FT   HELIX           2..15
FT                   /evidence="ECO:0007829|PDB:5JDX"
FT   STRAND          23..26
FT                   /evidence="ECO:0007829|PDB:5JDX"
FT   TURN            28..30
FT                   /evidence="ECO:0007829|PDB:5JDX"
FT   HELIX           36..49
FT                   /evidence="ECO:0007829|PDB:5JDX"
FT   TURN            56..58
FT                   /evidence="ECO:0007829|PDB:5JDX"
FT   TURN            61..63
FT                   /evidence="ECO:0007829|PDB:5JDX"
FT   STRAND          64..66
FT                   /evidence="ECO:0007829|PDB:5JDX"
FT   HELIX           67..77
FT                   /evidence="ECO:0007829|PDB:5JDX"
SQ   SEQUENCE   87 AA;  9536 MW;  F4A8EDA4E31DDF49 CRC64;
     MLESKLINHI ATQFLDGEKD GLDSQTPLFE LNIVDSAAIF DLVDFLRQES KVSIGMQEIH
     PANFATVQSM VALVQRLKAH PEQGGAA