PIGL_DICDI
ID PIGL_DICDI Reviewed; 258 AA.
AC Q54C64;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 03-AUG-2022, entry version 94.
DE RecName: Full=Probable N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase;
DE EC=3.5.1.89;
DE AltName: Full=Phosphatidylinositol-glycan biosynthesis class L protein;
DE Short=PIG-L;
GN Name=pigl; ORFNames=DDB_G0293136;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- FUNCTION: Involved in the second step of GPI biosynthesis. De-N-
CC acetylation of N-acetylglucosaminyl-phosphatidylinositol (By
CC similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-
CC inositol + H2O = acetate + an alpha-D-GlcN-(1->6)-(1,2-diacyl-sn-
CC glycero-3-phospho)-1D-myo-inositol; Xref=Rhea:RHEA:11660,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:30089, ChEBI:CHEBI:57265,
CC ChEBI:CHEBI:57997; EC=3.5.1.89;
CC -!- PATHWAY: Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor
CC biosynthesis.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the PIGL family. {ECO:0000305}.
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DR EMBL; AAFI02000199; EAL60920.1; -; Genomic_DNA.
DR RefSeq; XP_629355.1; XM_629353.1.
DR AlphaFoldDB; Q54C64; -.
DR SMR; Q54C64; -.
DR STRING; 44689.DDB0235227; -.
DR PaxDb; Q54C64; -.
DR EnsemblProtists; EAL60920; EAL60920; DDB_G0293136.
DR GeneID; 8629079; -.
DR KEGG; ddi:DDB_G0293136; -.
DR dictyBase; DDB_G0293136; pigL.
DR eggNOG; KOG3332; Eukaryota.
DR HOGENOM; CLU_034979_1_0_1; -.
DR InParanoid; Q54C64; -.
DR OMA; RWGWITI; -.
DR PhylomeDB; Q54C64; -.
DR Reactome; R-DDI-162710; Synthesis of glycosylphosphatidylinositol (GPI).
DR UniPathway; UPA00196; -.
DR PRO; PR:Q54C64; -.
DR Proteomes; UP000002195; Chromosome 6.
DR GO; GO:0005783; C:endoplasmic reticulum; IBA:GO_Central.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016811; F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides; IBA:GO_Central.
DR GO; GO:0000225; F:N-acetylglucosaminylphosphatidylinositol deacetylase activity; IBA:GO_Central.
DR GO; GO:0006506; P:GPI anchor biosynthetic process; IBA:GO_Central.
DR Gene3D; 3.40.50.10320; -; 1.
DR InterPro; IPR003737; GlcNAc_PI_deacetylase-related.
DR InterPro; IPR039516; GPI12.
DR InterPro; IPR024078; LmbE-like_dom_sf.
DR PANTHER; PTHR12993; PTHR12993; 1.
DR PANTHER; PTHR12993:SF11; PTHR12993:SF11; 1.
DR Pfam; PF02585; PIG-L; 1.
DR SUPFAM; SSF102588; SSF102588; 1.
PE 3: Inferred from homology;
KW Endoplasmic reticulum; GPI-anchor biosynthesis; Hydrolase; Membrane;
KW Reference proteome.
FT CHAIN 1..258
FT /note="Probable N-acetylglucosaminyl-phosphatidylinositol
FT de-N-acetylase"
FT /id="PRO_0000328582"
FT REGION 147..170
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 258 AA; 29237 MW; C83B099E30F24125 CRC64;
MKNHLNNNED NSTLIKKKVL FVIAHPDDEC MFFTPTIQHY NFIGSEIFVA CLSNGNAVGL
GKIREKELID SCIDMGINQE NVFFDQTNNF QDGMNIIWDT DLVEKTILSF IKQTSADIVI
SFDECGISSH PNHISISNGL KQLMKNKSSS TTTTSTTSSS SSSSSLSNRT TNNLNKEIKA
YKLETVNIIR KYIGIADIPL TKLLSYDENS TQTFISTQLF PPSSYSPMTK HKSQFVWFRY
LFVFLSRYSF INTLIEIK
