ID   PIGM_SERS3              Reviewed;         359 AA.
AC   Q5W259;
DT   11-MAY-2016, integrated into UniProtKB/Swiss-Prot.
DT   07-DEC-2004, sequence version 1.
DT   25-MAY-2022, entry version 34.
DE   RecName: Full=Probable NAD(P)H nitroreductase PigM {ECO:0000303|PubMed:15853884};
DE            EC=1.-.-.- {ECO:0000305|PubMed:15853884};
GN   Name=pigM {ECO:0000303|PubMed:15528645};
OS   Serratia sp. (strain ATCC 39006).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Yersiniaceae; Serratia; unclassified Serratia.
OX   NCBI_TaxID=104623;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 39006 / SC 11482;
RX   PubMed=15528645; DOI=10.1099/mic.0.27222-0;
RA   Harris A.K., Williamson N.R., Slater H., Cox A., Abbasi S., Foulds I.,
RA   Simonsen H.T., Leeper F.J., Salmond G.P.;
RT   "The Serratia gene cluster encoding biosynthesis of the red antibiotic,
RT   prodigiosin, shows species- and strain-dependent genome context
RT   variation.";
RL   Microbiology 150:3547-3560(2004).
RN   [2]
RP   FUNCTION, CATALYTIC ACTIVITY, DISRUPTION PHENOTYPE, COFACTOR, AND PATHWAY.
RC   STRAIN=ATCC 39006 / SC 11482;
RX   PubMed=15853884; DOI=10.1111/j.1365-2958.2005.04602.x;
RA   Williamson N.R., Simonsen H.T., Ahmed R.A., Goldet G., Slater H.,
RA   Woodley L., Leeper F.J., Salmond G.P.;
RT   "Biosynthesis of the red antibiotic, prodigiosin, in Serratia:
RT   identification of a novel 2-methyl-3-n-amyl-pyrrole (MAP) assembly pathway,
RT   definition of the terminal condensing enzyme, and implications for
RT   undecylprodigiosin biosynthesis in Streptomyces.";
RL   Mol. Microbiol. 56:971-989(2005).
CC   -!- FUNCTION: Involved in the biosynthesis of 4-methoxy-2,2'-bipyrrole-5-
CC       carbaldehyde (MBC), one of the terminal products involved in the
CC       biosynthesis of the red antibiotic prodigiosin (Pig). Catalyzes the
CC       oxidation of the hydroxy group of 4-hydroxy-2,2'-bipyrrole-5-methanol
CC       (HBM) to yield 4-methoxy-2,2'-bipyrrole-5-carbaldehyde (MBC).
CC       {ECO:0000269|PubMed:15853884}.
CC   -!- COFACTOR:
CC       Name=FMN; Xref=ChEBI:CHEBI:58210;
CC         Evidence={ECO:0000305|PubMed:15853884};
CC   -!- PATHWAY: Antibiotic biosynthesis; prodigiosin biosynthesis.
CC       {ECO:0000305|PubMed:15853884}.
CC   -!- DISRUPTION PHENOTYPE: Cells lacking this gene show a white phenotype,
CC       and produce 2-methyl-3-n-amyl-pyrrole (MAP) and 4-hydroxy-2,2'-
CC       bipyrrole-5-methanol (HBM). {ECO:0000269|PubMed:15853884}.
CC   -!- SIMILARITY: Belongs to the nitroreductase family. {ECO:0000305}.
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DR   EMBL; AJ833001; CAH55641.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q5W259; -.
DR   SMR; Q5W259; -.
DR   STRING; 104623.Ser39006_01381; -.
DR   KEGG; ag:CAH55641; -.
DR   eggNOG; COG0778; Bacteria.
DR   UniPathway; UPA01072; -.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   GO; GO:0017000; P:antibiotic biosynthetic process; IMP:UniProtKB.
DR   Gene3D; 3.40.109.10; -; 1.
DR   InterPro; IPR000415; Nitroreductase-like.
DR   SUPFAM; SSF55469; SSF55469; 1.
PE   1: Evidence at protein level;
KW   Flavoprotein; FMN; NAD; NADP; Oxidoreductase.
FT   CHAIN           1..359
FT                   /note="Probable NAD(P)H nitroreductase PigM"
FT                   /id="PRO_0000436248"
SQ   SEQUENCE   359 AA;  40860 MW;  FFBC24663178CE9D CRC64;
     MVNDTFEQAL QNAINIARLA PSSHNCQPWS VHYDAATRCG EVSIDRQRAL KGLPSLEREM
     LMSCGIFFEY LSTLLKHSGY PLDWQWVGAR QNGSSGMLIS FAPSAPCVAD LVAYQQWVQR
     ISDRHTVRTA YQPTQVNEQQ QAQLYALFDR SPVTCDIKYG EATRHDVAFL TANYASLDFA
     DQQAWRETYH YIRFNEQQAA EDGFYLHHLF GPVSCGFRWF FRIAFHPRLS WLAKRLRLPA
     SMAKGLAELV VEGPQYLALS LEHESDENLF IAGMKLGQLW LMLQSWGWSL HPLSVLVQHA
     TARCALADTV RLTGLPVFFA RFGQHRQSGI PTPRRAWQRI LTTTQHSFSP ENGADVKQP