ID   PIGP_ARATH              Reviewed;         137 AA.
AC   O64792; A6QRD5;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   03-AUG-2022, entry version 122.
DE   RecName: Full=Phosphatidylinositol N-acetylglucosaminyltransferase subunit P;
DE            EC=2.4.1.198;
GN   OrderedLocusNames=At1g61280; ORFNames=T1F9.23;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130712; DOI=10.1038/35048500;
RA   Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA   Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA   Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA   Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA   Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA   Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA   Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA   Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA   Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA   Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA   Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA   Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA   Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA   Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA   Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT   "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL   Nature 408:816-820(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RA   Bautista-Mercan V.R., Kim C.J., Chen H., Quan R., De Los Reyes C.,
RA   Ecker J.R.;
RT   "Arabidopsis ORF clones.";
RL   Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Part of the complex catalyzing the transfer of N-
CC       acetylglucosamine from UDP-N-acetylglucosamine to phosphatidylinositol,
CC       the first step of GPI biosynthesis. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + UDP-N-
CC         acetyl-alpha-D-glucosamine = a 6-(N-acetyl-alpha-D-glucosaminyl)-1-
CC         phosphatidyl-1D-myo-inositol + H(+) + UDP; Xref=Rhea:RHEA:14789,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57265, ChEBI:CHEBI:57705,
CC         ChEBI:CHEBI:57880, ChEBI:CHEBI:58223; EC=2.4.1.198;
CC   -!- PATHWAY: Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor
CC       biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the PIGP family. {ECO:0000305}.
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DR   EMBL; AC004255; AAC13913.1; -; Genomic_DNA.
DR   EMBL; CP002684; AEE33816.1; -; Genomic_DNA.
DR   EMBL; CP002684; ANM58168.1; -; Genomic_DNA.
DR   EMBL; BT030658; ABR46238.1; -; mRNA.
DR   RefSeq; NP_001319281.1; NM_001333947.1.
DR   RefSeq; NP_176323.1; NM_104809.2.
DR   AlphaFoldDB; O64792; -.
DR   STRING; 3702.AT1G61280.1; -.
DR   PaxDb; O64792; -.
DR   PRIDE; O64792; -.
DR   EnsemblPlants; AT1G61280.1; AT1G61280.1; AT1G61280.
DR   EnsemblPlants; AT1G61280.2; AT1G61280.2; AT1G61280.
DR   GeneID; 842422; -.
DR   Gramene; AT1G61280.1; AT1G61280.1; AT1G61280.
DR   Gramene; AT1G61280.2; AT1G61280.2; AT1G61280.
DR   KEGG; ath:AT1G61280; -.
DR   Araport; AT1G61280; -.
DR   TAIR; locus:2197755; AT1G61280.
DR   eggNOG; KOG2257; Eukaryota.
DR   HOGENOM; CLU_081616_0_1_1; -.
DR   InParanoid; O64792; -.
DR   OrthoDB; 1579862at2759; -.
DR   PhylomeDB; O64792; -.
DR   BioCyc; ARA:AT1G61280-MON; -.
DR   UniPathway; UPA00196; -.
DR   PRO; PR:O64792; -.
DR   Proteomes; UP000006548; Chromosome 1.
DR   ExpressionAtlas; O64792; baseline and differential.
DR   Genevisible; O64792; AT.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0017176; F:phosphatidylinositol N-acetylglucosaminyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006506; P:GPI anchor biosynthetic process; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR013717; PIG-P.
DR   InterPro; IPR016542; PIG-P_GPI19.
DR   Pfam; PF08510; PIG-P; 1.
DR   PIRSF; PIRSF008765; PIG-P_GPI19; 1.
PE   2: Evidence at transcript level;
KW   Glycosyltransferase; GPI-anchor biosynthesis; Membrane; Reference proteome;
KW   Transferase; Transmembrane; Transmembrane helix.
FT   CHAIN           1..137
FT                   /note="Phosphatidylinositol N-acetylglucosaminyltransferase
FT                   subunit P"
FT                   /id="PRO_0000191786"
FT   TRANSMEM        16..36
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        58..78
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   137 AA;  15611 MW;  ADEC3D4906CDF124 CRC64;
     MLSLNQEVHG PKTSEVYGFV GSISIVVATV IFLIWGYVPD KFLESIGIYY YPSKYWAMAM
     PMYSMVTLLV ALVFYIGLNF MSTSKPTSLN TLFDDYSRED VNFLPLMKNG EDRPIDPISD
     IDITRINDLM FDSHLAK