ID   PIGS_BOVIN              Reviewed;         555 AA.
AC   Q3SZL5;
DT   17-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   25-MAY-2022, entry version 66.
DE   RecName: Full=GPI transamidase component PIG-S;
DE   AltName: Full=Phosphatidylinositol-glycan biosynthesis class S protein;
GN   Name=PIGS;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Crossbred X Angus; TISSUE=Ileum;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Component of the GPI transamidase complex. Essential for
CC       transfer of GPI to proteins, particularly for formation of carbonyl
CC       intermediates (By similarity). {ECO:0000250}.
CC   -!- PATHWAY: Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor
CC       biosynthesis.
CC   -!- SUBUNIT: Forms a complex with PIGK/GPI8, PIGT, PIGU and GAA1.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250};
CC       Multi-pass membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the PIGS family. {ECO:0000305}.
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DR   EMBL; BC102800; AAI02801.1; -; mRNA.
DR   RefSeq; NP_001029388.1; NM_001034216.1.
DR   AlphaFoldDB; Q3SZL5; -.
DR   SMR; Q3SZL5; -.
DR   STRING; 9913.ENSBTAP00000017414; -.
DR   PaxDb; Q3SZL5; -.
DR   GeneID; 504583; -.
DR   KEGG; bta:504583; -.
DR   CTD; 94005; -.
DR   eggNOG; KOG2459; Eukaryota.
DR   InParanoid; Q3SZL5; -.
DR   OrthoDB; 1399804at2759; -.
DR   UniPathway; UPA00196; -.
DR   Proteomes; UP000009136; Unplaced.
DR   GO; GO:0042765; C:GPI-anchor transamidase complex; IEA:InterPro.
DR   GO; GO:0016255; P:attachment of GPI anchor to protein; IEA:InterPro.
DR   InterPro; IPR019540; PtdIno-glycan_biosynth_class_S.
DR   PANTHER; PTHR21072; PTHR21072; 1.
DR   Pfam; PF10510; PIG-S; 1.
PE   2: Evidence at transcript level;
KW   Endoplasmic reticulum; Glycoprotein; GPI-anchor biosynthesis; Membrane;
KW   Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..555
FT                   /note="GPI transamidase component PIG-S"
FT                   /id="PRO_0000253474"
FT   TOPO_DOM        1..18
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        19..39
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        40..520
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        521..541
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        542..555
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        267
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        370
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   555 AA;  61786 MW;  A07809034FB96171 CRC64;
     MAATGAAATD LEVVRGKRAA LFFATVVIVL GLPLWWKTTE TYRAPLPYSQ ISGLNSLKLR
     LMVPVTVVFT QESVPLDDQE KLPFTVVHER EIPLKYKLKI KCRFQKAYRR ALDHEEAALS
     LGNIQEAEAM LAEPSEQAEG SLTVYVISER CSLLPQDMMS YIGPKRMAVV RGITHREAFN
     IIGRRIIQVV QAMSLTEDVL AAALADHLPE DKWSSDKRRP LKSSLGYEIT FSLLNPDPKS
     HDVHWDIEGA VRRYVQPFLS ALSAAGNFSV DSQILYYAVL GVNPRFDSAS SSYYLAAHSL
     PHVINPVESR LGSSAASLYP VLNFLLYVPE LAHSPLYIQD KDGAPVATNA FHSPRWGGIM
     VYNVDPKAYN GSQLPVRVEV DMMRVMEVFL AQLRLLFGIA QPQLPPKCLF FGPKSEGIMT
     WELDRLLWAR SVENLATATT TLTSLAQLLG KISNIVIKDD VASEVYRAVA AVQKAAEELS
     SGHLASAFAA SQEAVTSSER AFFDPSLLHL LYFPDDQKFA IYIPLFLPMA VPILLSLFKI
     FLETRKSWKK PEKTD