PIGS_MOUSE
ID PIGS_MOUSE Reviewed; 555 AA.
AC Q6PD26;
DT 15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=GPI transamidase component PIG-S;
DE AltName: Full=Phosphatidylinositol-glycan biosynthesis class S protein;
GN Name=Pigs;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-370.
RX PubMed=19349973; DOI=10.1038/nbt.1532;
RA Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M.,
RA Schiess R., Aebersold R., Watts J.D.;
RT "Mass-spectrometric identification and relative quantification of N-linked
RT cell surface glycoproteins.";
RL Nat. Biotechnol. 27:378-386(2009).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas,
RC Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Component of the GPI transamidase complex. Essential for
CC transfer of GPI to proteins, particularly for formation of carbonyl
CC intermediates (By similarity). {ECO:0000250}.
CC -!- PATHWAY: Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor
CC biosynthesis.
CC -!- SUBUNIT: Forms a complex with PIGK/GPI8, PIGT, PIGU and GAA1.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250};
CC Multi-pass membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the PIGS family. {ECO:0000305}.
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DR EMBL; AL591070; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC058979; AAH58979.1; -; mRNA.
DR CCDS; CCDS25100.1; -.
DR RefSeq; NP_958808.1; NM_201406.1.
DR AlphaFoldDB; Q6PD26; -.
DR SMR; Q6PD26; -.
DR BioGRID; 234902; 2.
DR IntAct; Q6PD26; 1.
DR STRING; 10090.ENSMUSP00000044871; -.
DR GlyConnect; 2360; 5 N-Linked glycans (1 site).
DR GlyGen; Q6PD26; 2 sites, 5 N-linked glycans (1 site).
DR iPTMnet; Q6PD26; -.
DR PhosphoSitePlus; Q6PD26; -.
DR EPD; Q6PD26; -.
DR jPOST; Q6PD26; -.
DR MaxQB; Q6PD26; -.
DR PaxDb; Q6PD26; -.
DR PRIDE; Q6PD26; -.
DR ProteomicsDB; 288211; -.
DR Antibodypedia; 2984; 118 antibodies from 24 providers.
DR Ensembl; ENSMUST00000048073; ENSMUSP00000044871; ENSMUSG00000041958.
DR GeneID; 276846; -.
DR KEGG; mmu:276846; -.
DR UCSC; uc007kiz.1; mouse.
DR CTD; 94005; -.
DR MGI; MGI:2687325; Pigs.
DR VEuPathDB; HostDB:ENSMUSG00000041958; -.
DR eggNOG; KOG2459; Eukaryota.
DR GeneTree; ENSGT00390000017203; -.
DR HOGENOM; CLU_010026_3_0_1; -.
DR InParanoid; Q6PD26; -.
DR OMA; AEHKYAV; -.
DR OrthoDB; 1399804at2759; -.
DR PhylomeDB; Q6PD26; -.
DR TreeFam; TF105857; -.
DR Reactome; R-MMU-162791; Attachment of GPI anchor to uPAR.
DR UniPathway; UPA00196; -.
DR BioGRID-ORCS; 276846; 16 hits in 75 CRISPR screens.
DR ChiTaRS; Pigs; mouse.
DR PRO; PR:Q6PD26; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; Q6PD26; protein.
DR Bgee; ENSMUSG00000041958; Expressed in choroid plexus of fourth ventricle and 252 other tissues.
DR ExpressionAtlas; Q6PD26; baseline and differential.
DR Genevisible; Q6PD26; MM.
DR GO; GO:0042765; C:GPI-anchor transamidase complex; IDA:UniProtKB.
DR GO; GO:0016255; P:attachment of GPI anchor to protein; IMP:UniProtKB.
DR InterPro; IPR019540; PtdIno-glycan_biosynth_class_S.
DR PANTHER; PTHR21072; PTHR21072; 1.
DR Pfam; PF10510; PIG-S; 1.
PE 1: Evidence at protein level;
KW Endoplasmic reticulum; Glycoprotein; GPI-anchor biosynthesis; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..555
FT /note="GPI transamidase component PIG-S"
FT /id="PRO_0000218605"
FT TOPO_DOM 1..18
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 19..39
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 40..520
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 521..541
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 542..555
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 267
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 370
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:19349973"
SQ SEQUENCE 555 AA; 61711 MW; 79B6599AD98659DA CRC64;
MAAAGAAATD LEVVRGKRSA LFFAAVAILL GLPLWWKTTE TYRAPLPYSD ISGLNALLLR
LMVPVTVVFT RDSVPLDDQE KLPFTVVHER EIPLKYKMKI KCRFQKAYRR ALEHEEEALS
LGSVHEAEAM LAEPEKQAEG SLTVYVISEH SSLLPQDMMS YIGPERTAVV RGLIHREAFN
IIGRRIVQVA QAMSLTEDVL AAALADHLPE DKWSSDKRRP LKSSLGYEIT FSLLNPDPKS
HDVHWDIEGA VQRFVQPFLN RLSVAGNFSV DSQILYYAML GVNPRFDPAS SSYSLAMHSL
PHVINPVESR LGSSAASLYP VLHFLLYVPE LAHSPLYIQD KDGAPVATNA FHSPRWGGIM
VYNVDPKIYN ASELPVRVEV DMVRVMEVFL AQLRLLFGIA QPQVPPKCLL SGPKSEGLMT
WELDRLLWAR SVENLATATT TLTSLAQLLG KISNIVIKDD VASEVYRAVA AVQKAAKALA
LGHLSSAFAA SQEAVTSSER AFFDPSLLHL LYFPDDQKFA IYIPLFLPMA VPILLSLVKI
FQETRKSWKK PEKID
