PIGS_SCHPO
ID PIGS_SCHPO Reviewed; 554 AA.
AC Q10351;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=GPI transamidase component PIG-S homolog;
GN Name=gpi17; ORFNames=SPAC1F12.09;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
CC -!- FUNCTION: Component of the GPI transamidase complex. Involved in
CC transfer of GPI to proteins (By similarity). {ECO:0000250}.
CC -!- PATHWAY: Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor
CC biosynthesis.
CC -!- SUBUNIT: Forms a complex with PIG-T homolog, PIG-U homolog and GPI8.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000269|PubMed:16823372}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:16823372}.
CC -!- SIMILARITY: Belongs to the PIGS family. {ECO:0000305}.
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DR EMBL; CU329670; CAA93813.1; -; Genomic_DNA.
DR PIR; S67452; S67452.
DR RefSeq; NP_594335.1; NM_001019756.2.
DR AlphaFoldDB; Q10351; -.
DR SMR; Q10351; -.
DR STRING; 4896.SPAC1F12.09.1; -.
DR MaxQB; Q10351; -.
DR PaxDb; Q10351; -.
DR EnsemblFungi; SPAC1F12.09.1; SPAC1F12.09.1:pep; SPAC1F12.09.
DR GeneID; 2542284; -.
DR KEGG; spo:SPAC1F12.09; -.
DR PomBase; SPAC1F12.09; gpi17.
DR VEuPathDB; FungiDB:SPAC1F12.09; -.
DR eggNOG; KOG2459; Eukaryota.
DR HOGENOM; CLU_010026_3_1_1; -.
DR InParanoid; Q10351; -.
DR OMA; WGGIFIY; -.
DR PhylomeDB; Q10351; -.
DR UniPathway; UPA00196; -.
DR PRO; PR:Q10351; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0005783; C:endoplasmic reticulum; HDA:PomBase.
DR GO; GO:0042765; C:GPI-anchor transamidase complex; ISO:PomBase.
DR GO; GO:0016255; P:attachment of GPI anchor to protein; ISO:PomBase.
DR InterPro; IPR019540; PtdIno-glycan_biosynth_class_S.
DR PANTHER; PTHR21072; PTHR21072; 1.
DR Pfam; PF10510; PIG-S; 1.
PE 3: Inferred from homology;
KW Endoplasmic reticulum; Glycoprotein; GPI-anchor biosynthesis; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..554
FT /note="GPI transamidase component PIG-S homolog"
FT /id="PRO_0000218607"
FT TOPO_DOM 1..73
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 74..94
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 95..511
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 512..532
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 533..554
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 132
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 375
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 554 AA; 63962 MW; 733014F3ED31C47A CRC64;
MSPCKWLTMF LKGCWGKIAN MNHLDSCFPL RYIIGRKKAS KLLCTMDSGL KSDSHEVERS
NFQLNKPEKS LKRYALLSFY VIILLAIPVW WKTTHYERSS LPFEDMENAP STVQTHLRFS
PTFRILDDKG NNLTKEVQKV LEAEPQIYSY NLKVLEDDPV DYRIVLREST DLQWFWDENN
FIIDTPSKGP SELAILIVNC LWEAFSPQVM EVWSKFTRFS STVEPSRAET KRTVQFSPQY
RVLLSLLVGE GNHEPINWDI ENAIQKYFNP LIEQLASLAK LNIETQIQYF VEDAEAYIKD
DKFCTKHADL PNLVNNFEKY LSFSPHIREP TIHFVLYVPS PQIQPLWLEN EDSNIIPTNS
MLLPQWGSIT TINFNVTEKK LLHDVDLKDY FRVISRDLLL LLGINDVPVS SLSATLADRL
LRQRIAESCI EASDTLQNLA KLVHSMQSMA VPKEIQMYVK DTLLSLDMAY KALSQNNLNE
ALSYSNNAFS KSQEALFHPS MVTTIYFPDE SKYGIYAPLF APILIPLLIS FIKEVKDMLR
ERKLHRVANV PKPN
