PIGW_MOUSE
ID PIGW_MOUSE Reviewed; 503 AA.
AC Q8C398; Q8C4S0; Q9CSX1;
DT 25-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=Phosphatidylinositol-glycan biosynthesis class W protein {ECO:0000305};
DE Short=PIG-W {ECO:0000305};
DE EC=2.3.-.- {ECO:0000250|UniProtKB:Q7TSN4};
GN Name=Pigw {ECO:0000312|MGI:MGI:1917575};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Head;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
CC -!- FUNCTION: Required for the transport of GPI-anchored proteins to the
CC plasma membrane. Probable acetyltransferase, which acetylates the
CC inositol ring of phosphatidylinositol during biosynthesis of GPI-
CC anchor. Acetylation during GPI-anchor biosynthesis is not essential for
CC the subsequent mannosylation and is usually removed soon after the
CC attachment of GPIs to proteins. {ECO:0000250|UniProtKB:Q7TSN4}.
CC -!- PATHWAY: Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor
CC biosynthesis. {ECO:0000250|UniProtKB:Q7TSN4}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000250|UniProtKB:Q7TSN4}; Multi-pass membrane protein
CC {ECO:0000250|UniProtKB:Q7TSN4}.
CC -!- SIMILARITY: Belongs to the PIGW family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC38199.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AK011762; BAB27826.3; -; mRNA.
DR EMBL; AK081337; BAC38199.1; ALT_FRAME; mRNA.
DR EMBL; AK086537; BAC39687.1; -; mRNA.
DR EMBL; AL645623; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS36263.1; -.
DR RefSeq; NP_001071104.1; NM_001077636.1.
DR RefSeq; NP_081664.2; NM_027388.2.
DR RefSeq; XP_006534222.1; XM_006534159.2.
DR AlphaFoldDB; Q8C398; -.
DR STRING; 10090.ENSMUSP00000064547; -.
DR GlyGen; Q8C398; 1 site.
DR PhosphoSitePlus; Q8C398; -.
DR PaxDb; Q8C398; -.
DR PRIDE; Q8C398; -.
DR ProteomicsDB; 288162; -.
DR Antibodypedia; 74148; 100 antibodies from 23 providers.
DR DNASU; 70325; -.
DR Ensembl; ENSMUST00000067058; ENSMUSP00000064547; ENSMUSG00000045140.
DR Ensembl; ENSMUST00000108080; ENSMUSP00000103715; ENSMUSG00000045140.
DR GeneID; 70325; -.
DR KEGG; mmu:70325; -.
DR UCSC; uc007kqy.1; mouse.
DR CTD; 284098; -.
DR MGI; MGI:1917575; Pigw.
DR VEuPathDB; HostDB:ENSMUSG00000045140; -.
DR eggNOG; KOG0411; Eukaryota.
DR GeneTree; ENSGT00390000013520; -.
DR HOGENOM; CLU_020802_2_2_1; -.
DR InParanoid; Q8C398; -.
DR OMA; GLYVMQP; -.
DR OrthoDB; 1202772at2759; -.
DR PhylomeDB; Q8C398; -.
DR TreeFam; TF314687; -.
DR Reactome; R-MMU-162710; Synthesis of glycosylphosphatidylinositol (GPI).
DR UniPathway; UPA00196; -.
DR BioGRID-ORCS; 70325; 10 hits in 76 CRISPR screens.
DR PRO; PR:Q8C398; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; Q8C398; protein.
DR Bgee; ENSMUSG00000045140; Expressed in epiblast (generic) and 62 other tissues.
DR ExpressionAtlas; Q8C398; baseline and differential.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0032216; F:glucosaminyl-phosphatidylinositol O-acyltransferase activity; IBA:GO_Central.
DR GO; GO:0006506; P:GPI anchor biosynthetic process; IBA:GO_Central.
DR GO; GO:0072659; P:protein localization to plasma membrane; ISS:UniProtKB.
DR InterPro; IPR009447; PIGW/GWT1.
DR PANTHER; PTHR20661; PTHR20661; 1.
DR Pfam; PF06423; GWT1; 1.
DR PIRSF; PIRSF017321; GWT1; 1.
PE 2: Evidence at transcript level;
KW Acyltransferase; Endoplasmic reticulum; Glycoprotein;
KW GPI-anchor biosynthesis; Membrane; Phosphoprotein; Reference proteome;
KW Transferase; Transmembrane; Transmembrane helix.
FT CHAIN 1..503
FT /note="Phosphatidylinositol-glycan biosynthesis class W
FT protein"
FT /id="PRO_0000246283"
FT TOPO_DOM 1..21
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 22..42
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 61..81
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 132..152
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 161..181
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 202..222
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 237..257
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 260..280
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 305..325
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 340..360
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 381..401
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 448..468
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 473..493
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MOD_RES 415
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q7Z7B1"
FT CARBOHYD 13
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 503 AA; 56841 MW; 3814E3D18680BACF CRC64;
MSQKQLKEAF VRNLSGTSVL EVTQGLCFPA FCILCRGLWI IFSQHVCSFS NTWSTRFLMD
FVVLIVPLVI TLTVLSSFIL LENLTVIVWG AWLLYQIYHR RTCYAKVPVQ KVFANFLKIS
LESEYNPAIT CYRVINSVFT AIAILAVDFP LFPRRFAKTE LYGTGAMDFG VGGFIFGAAM
VCPEVRRKSI EESRFNYLRK SLYSVWPLVF LGMGRLVIIK SIGYQEHSTE YGIHWNFFFT
IIVVRLVTSL LLIIFPLNKS WIVAVSITVV YQLALDYTPL KRILLYGTDG SGTRVGFLNA
NREGIISTLG YVTIHMAGVQ TGLYVLKGRA QVRDWIKATC WVFSVAVGFF ISLHIVQVNI
EAVSRRMANL AFCLWVVASS LMLLSCLLLS GIILSFAQFL IKGSLVPCSW KLIQSPTTHK
NHSESLILEA EKNQPSLCLI TALNRNQLFF FLLSNITTGL INLTMDTLHT GALWTLVVLS
IYMFTNCLVI YVLDLQGKTI KFW
