PIGX_MOUSE
ID PIGX_MOUSE Reviewed; 254 AA.
AC Q99LV7;
DT 25-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT 25-JUL-2006, sequence version 2.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=Phosphatidylinositol-glycan biosynthesis class X protein;
DE Short=PIG-X;
DE Flags: Precursor;
GN Name=Pigx;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Small intestine;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=FVB/N; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP INITIATION CODON CTG.
RX PubMed=15635094; DOI=10.1091/mbc.e04-09-0802;
RA Ashida H., Hong Y., Murakami Y., Shishioh N., Sugimoto N., Kim Y.U.,
RA Maeda Y., Kinoshita T.;
RT "Mammalian PIG-X and yeast Pbn1p are the essential components of
RT glycosylphosphatidylinositol-mannosyltransferase I.";
RL Mol. Biol. Cell 16:1439-1448(2005).
CC -!- FUNCTION: Essential component of glycosylphosphatidylinositol-
CC mannosyltransferase 1 which transfers the first of the 4 mannoses in
CC the GPI-anchor precursors during GPI-anchor biosynthesis. Probably acts
CC by stabilizing the mannosyltransferase PIGM (By similarity).
CC {ECO:0000250}.
CC -!- PATHWAY: Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor
CC biosynthesis.
CC -!- SUBUNIT: Interacts with PIGM. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250};
CC Single-pass type I membrane protein {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q99LV7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q99LV7-2; Sequence=VSP_019843;
CC -!- PTM: N-glycosylated. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the PIGX family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC25223.1; Type=Miscellaneous discrepancy; Note=Unusual initiator. The initiator methionine is coded by a non-canonical CTG leucine codon.; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AK008583; BAC25223.1; ALT_SEQ; mRNA.
DR EMBL; BC002202; AAH02202.1; -; mRNA.
DR CCDS; CCDS28113.2; -. [Q99LV7-1]
DR RefSeq; NP_077784.2; NM_024464.3. [Q99LV7-1]
DR AlphaFoldDB; Q99LV7; -.
DR SMR; Q99LV7; -.
DR IntAct; Q99LV7; 1.
DR STRING; 10090.ENSMUSP00000093819; -.
DR GlyGen; Q99LV7; 1 site.
DR PhosphoSitePlus; Q99LV7; -.
DR EPD; Q99LV7; -.
DR MaxQB; Q99LV7; -.
DR PaxDb; Q99LV7; -.
DR PeptideAtlas; Q99LV7; -.
DR PRIDE; Q99LV7; -.
DR ProteomicsDB; 287726; -. [Q99LV7-1]
DR ProteomicsDB; 287727; -. [Q99LV7-2]
DR Antibodypedia; 33945; 145 antibodies from 24 providers.
DR DNASU; 72084; -.
DR Ensembl; ENSMUST00000096109; ENSMUSP00000093819; ENSMUSG00000023791. [Q99LV7-1]
DR Ensembl; ENSMUST00000232321; ENSMUSP00000156144; ENSMUSG00000023791. [Q99LV7-2]
DR GeneID; 72084; -.
DR KEGG; mmu:72084; -.
DR UCSC; uc007yye.3; mouse. [Q99LV7-1]
DR CTD; 54965; -.
DR MGI; MGI:1919334; Pigx.
DR VEuPathDB; HostDB:ENSMUSG00000023791; -.
DR eggNOG; ENOG502S32M; Eukaryota.
DR GeneTree; ENSGT00390000017679; -.
DR InParanoid; Q99LV7; -.
DR OMA; SKYMWKH; -.
DR OrthoDB; 1537024at2759; -.
DR PhylomeDB; Q99LV7; -.
DR Reactome; R-MMU-162710; Synthesis of glycosylphosphatidylinositol (GPI).
DR UniPathway; UPA00196; -.
DR BioGRID-ORCS; 72084; 5 hits in 74 CRISPR screens.
DR ChiTaRS; Pigx; mouse.
DR PRO; PR:Q99LV7; -.
DR Proteomes; UP000000589; Chromosome 16.
DR RNAct; Q99LV7; protein.
DR Bgee; ENSMUSG00000023791; Expressed in metanephric ureteric bud and 259 other tissues.
DR ExpressionAtlas; Q99LV7; baseline and differential.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0006506; P:GPI anchor biosynthetic process; IEA:UniProtKB-UniPathway.
DR InterPro; IPR013233; PIG-X/PBN1.
DR InterPro; IPR040039; PIGX.
DR PANTHER; PTHR28650; PTHR28650; 1.
DR Pfam; PF08320; PIG-X; 1.
DR SMART; SM00780; PIG-X; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Endoplasmic reticulum; Glycoprotein;
KW GPI-anchor biosynthesis; Membrane; Reference proteome; Signal;
KW Transmembrane; Transmembrane helix.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..254
FT /note="Phosphatidylinositol-glycan biosynthesis class X
FT protein"
FT /id="PRO_0000246296"
FT TOPO_DOM 23..226
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 227..247
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 248..254
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 211
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..104
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_019843"
SQ SEQUENCE 254 AA; 28644 MW; B2724144EFAD41D7 CRC64;
MAAGAVAWLL LWAAWLVGRL AADFSDAPFS AGKGVRATCS EIILRQEFLK DGFHRDLLIK
VKFGESIEDL QTCRLLIKHY IPPGLFVDPY ELASLRERNL TEAVMLSESF NIEAPNYLSN
ESAVLIYARQ DAQCIDCFQA FLPVHYRYHR PHKKDGDTLI VVNNPDLLMY CDQEFPILKC
WAQSEVAAPC ALKSEEICQW KSMQYKSILK NLTVQVPVGL TIHTSLVCSV TLLITILCST
LILLAVFKYG HFSL
