PIGY_MOUSE
ID PIGY_MOUSE Reviewed; 71 AA.
AC P0C1P0;
DT 25-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT 25-JUL-2006, sequence version 1.
DT 03-AUG-2022, entry version 81.
DE RecName: Full=Phosphatidylinositol N-acetylglucosaminyltransferase subunit Y {ECO:0000305};
DE AltName: Full=Phosphatidylinositol-glycan biosynthesis class Y protein;
DE Short=PIG-Y;
GN Name=Pigy {ECO:0000312|MGI:MGI:1913518}; Synonyms=Pigyl;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Corpus striatum;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
CC -!- FUNCTION: Part of the glycosylphosphatidylinositol-N-
CC acetylglucosaminyltransferase (GPI-GnT) complex that catalyzes the
CC transfer of N-acetylglucosamine from UDP-N-acetylglucosamine to
CC phosphatidylinositol and participates in the first step of GPI
CC biosynthesis. May act by regulating the catalytic subunit PIGA.
CC {ECO:0000250|UniProtKB:Q3MUY2}.
CC -!- PATHWAY: Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor
CC biosynthesis. {ECO:0000250|UniProtKB:Q3MUY2}.
CC -!- SUBUNIT: Component of the glycosylphosphatidylinositol-N-
CC acetylglucosaminyltransferase (GPI-GnT) complex composed at least by
CC PIGA, PIGC, PIGH, PIGP, PIGQ, PIGY and DPM2. Interacts directly with
CC PIGA; this interaction regulates glycosylphosphatidylinositol-N-
CC acetylglucosaminyltransferase activity. Does not interact with Ras
CC proteins. {ECO:0000250|UniProtKB:Q3MUY2}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000250|UniProtKB:Q3MUY2}; Multi-pass membrane protein
CC {ECO:0000250|UniProtKB:Q3MUY2}.
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DR EMBL; AK021110; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; AC159308; -; NOT_ANNOTATED_CDS; mRNA.
DR CCDS; CCDS52742.1; -.
DR RefSeq; NP_001076001.1; NM_001082532.1.
DR AlphaFoldDB; P0C1P0; -.
DR STRING; 10090.ENSMUSP00000116929; -.
DR iPTMnet; P0C1P0; -.
DR PhosphoSitePlus; P0C1P0; -.
DR PaxDb; P0C1P0; -.
DR PRIDE; P0C1P0; -.
DR Antibodypedia; 58497; 80 antibodies from 21 providers.
DR Ensembl; ENSMUST00000123680; ENSMUSP00000116929; ENSMUSG00000010607.
DR Ensembl; ENSMUST00000148088; ENSMUSP00000116967; ENSMUSG00000010607.
DR GeneID; 66268; -.
DR KEGG; mmu:66268; -.
DR UCSC; uc009ooc.1; mouse.
DR CTD; 66268; -.
DR MGI; MGI:1913518; Pigyl.
DR VEuPathDB; HostDB:ENSMUSG00000010607; -.
DR eggNOG; ENOG502S4A7; Eukaryota.
DR GeneTree; ENSGT00610000087446; -.
DR HOGENOM; CLU_2978556_0_0_1; -.
DR InParanoid; P0C1P0; -.
DR OMA; DETFPQG; -.
DR OrthoDB; 1610963at2759; -.
DR PhylomeDB; P0C1P0; -.
DR Reactome; R-MMU-162710; Synthesis of glycosylphosphatidylinositol (GPI).
DR UniPathway; UPA00196; -.
DR BioGRID-ORCS; 66268; 6 hits in 74 CRISPR screens.
DR PRO; PR:P0C1P0; -.
DR Proteomes; UP000000589; Chromosome 9.
DR RNAct; P0C1P0; protein.
DR Bgee; ENSMUSG00000010607; Expressed in right kidney and 231 other tissues.
DR Genevisible; P0C1P0; MM.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; ISO:MGI.
DR GO; GO:0000506; C:glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex; ISO:MGI.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0017176; F:phosphatidylinositol N-acetylglucosaminyltransferase activity; ISO:MGI.
DR GO; GO:0006506; P:GPI anchor biosynthetic process; IBA:GO_Central.
DR InterPro; IPR029164; PIG-Y.
DR InterPro; IPR033535; PIGY_chordates.
DR PANTHER; PTHR39235; PTHR39235; 1.
DR Pfam; PF15159; PIG-Y; 1.
PE 3: Inferred from homology;
KW Endoplasmic reticulum; GPI-anchor biosynthesis; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..71
FT /note="Phosphatidylinositol N-acetylglucosaminyltransferase
FT subunit Y"
FT /id="PRO_0000246312"
FT TOPO_DOM 1..5
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 6..26
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 27..44
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 45..65
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 66..71
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 71 AA; 7952 MW; 88CC7F21C2CCF5B6 CRC64;
MIRSLPTMTV LIPLVSLAGL LYSASVEEGF PEGCTSASSL CFYSLLLPVT VPVYVFFHLW
TWMGLKLFRH N
