PIGZ_DROME
ID PIGZ_DROME Reviewed; 696 AA.
AC Q8MT80; A0A0B4LGH6; Q9W176;
DT 25-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=GPI mannosyltransferase 4;
DE EC=2.4.1.-;
DE AltName: Full=GPI mannosyltransferase IV;
DE Short=GPI-MT-IV;
DE AltName: Full=Phosphatidylinositol glycan anchor biosynthesis protein class Z {ECO:0000312|FlyBase:FBgn0266438};
GN Name=PIG-Z {ECO:0000312|FlyBase:FBgn0266438};
GN ORFNames=CG45068 {ECO:0000312|FlyBase:FBgn0266438};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC -!- FUNCTION: Mannosyltransferase involved in glycosylphosphatidylinositol-
CC anchor biosynthesis. Transfers a fourth mannose to some trimannosyl-
CC GPIs during GPI precursor assembly (By similarity). {ECO:0000250}.
CC -!- PATHWAY: Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor
CC biosynthesis.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250};
CC Multi-pass membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 22 family. PIGZ
CC subfamily. {ECO:0000305}.
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DR EMBL; AE013599; AHN56635.1; -; Genomic_DNA.
DR EMBL; AY118320; AAM48349.1; -; mRNA.
DR RefSeq; NP_001286840.1; NM_001299911.1.
DR AlphaFoldDB; Q8MT80; -.
DR BioGRID; 2594345; 3.
DR STRING; 7227.FBpp0072198; -.
DR CAZy; GT22; Glycosyltransferase Family 22.
DR PaxDb; Q8MT80; -.
DR PRIDE; Q8MT80; -.
DR DNASU; 19835548; -.
DR EnsemblMetazoa; FBtr0344430; FBpp0310802; FBgn0266438.
DR GeneID; 19835548; -.
DR KEGG; dme:Dmel_CG45068; -.
DR UCSC; CG3419-RA; d. melanogaster.
DR CTD; 19835548; -.
DR FlyBase; FBgn0266438; PIG-Z.
DR VEuPathDB; VectorBase:FBgn0266438; -.
DR eggNOG; KOG4123; Eukaryota.
DR GeneTree; ENSGT00950000183090; -.
DR HOGENOM; CLU_022957_1_0_1; -.
DR InParanoid; Q8MT80; -.
DR OMA; YRICRLY; -.
DR PhylomeDB; Q8MT80; -.
DR Reactome; R-DME-162710; Synthesis of glycosylphosphatidylinositol (GPI).
DR UniPathway; UPA00196; -.
DR BioGRID-ORCS; 19835548; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 19835548; -.
DR PRO; PR:Q8MT80; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0266438; Expressed in ovary and 6 other tissues.
DR Genevisible; Q8MT80; DM.
DR GO; GO:0005783; C:endoplasmic reticulum; ISS:UniProtKB.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0000026; F:alpha-1,2-mannosyltransferase activity; ISS:UniProtKB.
DR GO; GO:0000030; F:mannosyltransferase activity; ISS:FlyBase.
DR GO; GO:0006506; P:GPI anchor biosynthetic process; ISS:UniProtKB.
DR InterPro; IPR005599; GPI_mannosylTrfase.
DR PANTHER; PTHR22760; PTHR22760; 1.
DR Pfam; PF03901; Glyco_transf_22; 1.
PE 2: Evidence at transcript level;
KW Endoplasmic reticulum; Glycosyltransferase; GPI-anchor biosynthesis;
KW Membrane; Reference proteome; Transferase; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..696
FT /note="GPI mannosyltransferase 4"
FT /id="PRO_0000246270"
FT TRANSMEM 100..120
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 125..142
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 149..169
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 185..205
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 227..247
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 338..358
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 696 AA; 80217 MW; DCB78A0FEE0E5799 CRC64;
MRLTTLWQNR SPGDRHLSTY FCFAAVRLLL VFVPQLGYVH PDEFFQSVEV MTGDHFRLEH
TRTWEFNNSL PVRSIVLPFA LLRIPWSFYE FVAECLKAWW QLELLGTYAY VVFPRLIYTL
ISFSNDYCLF RICRLYGLRF EIRLLAMGSS WILLVFGTRT FSNSLEMAMC SWLLCLVSEC
MLRTNTVVYK KEFLEEKYDK AESISERVRI WKLKNSLPAH NLQHLMAMST ICVAGVFNRP
TFLLFGAPMV FFWLLRGMGT RSITFRDFNL RIALFCLCAL PALVLFIFCD SLYYQHLTLG
ELHMMHLSID NFVFTPWNFI KANLDSAQTA SHGVHPCYVH LMVNMPMLFN VLALASLGAF
AQLLLRFFRA EYQVLPRFQS IVSLMSGAIF VPLFFLSLIN HQEPRFLLPV TFPLILLHAP
KLITGFSAKY PFQKDHPLLR LFYDKLLSSK ASGPYLLKIW YVSNVALTLF FGFIHQAGVY
PLAADISHVI ATKPAATHVH LITSHIYSLP LHLINIPSSR VLHFNRLTHQ RYRRPRDFYM
YEYGGLPLDS LLQKVKLISG SCEVKKSGPS RRRYKLYLAI PASLSADLHE ALVHSNASSY
LNFELLKVFY PHLSTEAFPH LQGRHPCDVD APHWAHDDLR GTCSAEQLPA FSFAYLNKQF
SSFVHQLGLA LYEIDVTRNK PRSVVIKKAS MTETAA
