PIGZ_MOUSE
ID PIGZ_MOUSE Reviewed; 560 AA.
AC Q8BTP0; Q149K0;
DT 25-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 2.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=GPI mannosyltransferase 4;
DE EC=2.4.1.-;
DE AltName: Full=GPI mannosyltransferase IV;
DE Short=GPI-MT-IV;
DE AltName: Full=Phosphatidylinositol-glycan biosynthesis class Z protein;
DE Short=PIG-Z;
GN Name=Pigz;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=NOD;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Mannosyltransferase involved in glycosylphosphatidylinositol-
CC anchor biosynthesis. Transfers a fourth mannose to some trimannosyl-
CC GPIs during GPI precursor assembly. The presence of a fourth mannose in
CC GPI is facultative and only scarcely detected, suggesting that it only
CC exists in some tissues (By similarity). {ECO:0000250}.
CC -!- PATHWAY: Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor
CC biosynthesis.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250};
CC Multi-pass membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 22 family. PIGZ
CC subfamily. {ECO:0000305}.
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DR EMBL; AK089183; BAC40783.1; -; mRNA.
DR EMBL; BC117748; AAI17749.1; -; mRNA.
DR CCDS; CCDS28109.1; -.
DR RefSeq; NP_766410.2; NM_172822.3.
DR RefSeq; XP_006522181.1; XM_006522118.3.
DR RefSeq; XP_006522182.1; XM_006522119.3.
DR RefSeq; XP_006522183.1; XM_006522120.3.
DR AlphaFoldDB; Q8BTP0; -.
DR STRING; 10090.ENSMUSP00000057509; -.
DR CAZy; GT22; Glycosyltransferase Family 22.
DR iPTMnet; Q8BTP0; -.
DR PhosphoSitePlus; Q8BTP0; -.
DR PaxDb; Q8BTP0; -.
DR PRIDE; Q8BTP0; -.
DR ProteomicsDB; 289422; -.
DR DNASU; 239827; -.
DR Ensembl; ENSMUST00000052174; ENSMUSP00000057509; ENSMUSG00000045625.
DR GeneID; 239827; -.
DR KEGG; mmu:239827; -.
DR UCSC; uc007yxx.2; mouse.
DR CTD; 80235; -.
DR MGI; MGI:2443822; Pigz.
DR VEuPathDB; HostDB:ENSMUSG00000045625; -.
DR eggNOG; KOG4123; Eukaryota.
DR GeneTree; ENSGT00950000183090; -.
DR HOGENOM; CLU_022957_1_1_1; -.
DR InParanoid; Q8BTP0; -.
DR OMA; VLWWKTY; -.
DR OrthoDB; 821144at2759; -.
DR PhylomeDB; Q8BTP0; -.
DR TreeFam; TF324461; -.
DR Reactome; R-MMU-162710; Synthesis of glycosylphosphatidylinositol (GPI).
DR UniPathway; UPA00196; -.
DR BioGRID-ORCS; 239827; 6 hits in 74 CRISPR screens.
DR ChiTaRS; Pigz; mouse.
DR PRO; PR:Q8BTP0; -.
DR Proteomes; UP000000589; Chromosome 16.
DR RNAct; Q8BTP0; protein.
DR Bgee; ENSMUSG00000045625; Expressed in lumbar subsegment of spinal cord and 88 other tissues.
DR ExpressionAtlas; Q8BTP0; baseline and differential.
DR Genevisible; Q8BTP0; MM.
DR GO; GO:0005783; C:endoplasmic reticulum; ISO:MGI.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0000026; F:alpha-1,2-mannosyltransferase activity; ISO:MGI.
DR GO; GO:0000030; F:mannosyltransferase activity; ISO:MGI.
DR GO; GO:0006506; P:GPI anchor biosynthetic process; ISO:MGI.
DR InterPro; IPR005599; GPI_mannosylTrfase.
DR PANTHER; PTHR22760; PTHR22760; 1.
DR Pfam; PF03901; Glyco_transf_22; 1.
PE 2: Evidence at transcript level;
KW Endoplasmic reticulum; Glycosyltransferase; GPI-anchor biosynthesis;
KW Membrane; Reference proteome; Transferase; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..560
FT /note="GPI mannosyltransferase 4"
FT /id="PRO_0000246269"
FT TRANSMEM 102..122
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 126..143
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 147..167
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 186..206
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 225..245
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 333..353
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 363..383
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 388..408
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 472
FT /note="N -> D (in Ref. 1; BAC40783)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 560 AA; 62043 MW; E7CAD2634D9AA159 CRC64;
MMVAAQVIWG SFSLLRVLWC LLPQTGYIHP DEFFQSPEVM AEDILGVQAT RPWEFYPSSS
CRTVVFPLLT SGSTFWLLRL WEELGLWPGL VSGYMLLVGP RFLLTALSFA LDWAVYDLAP
LWGADRWNAL CLLSGSYVTL VFYTRTFSNT IEGLLFTWLL VLVSPGVVRS PTSKKPTPGP
RWHRYLLGVI LAAGFFNRPT FLAFALAPLS LWGIHRASEL GGIRALIQEA LVLLPGAALA
AVLCVATDSW YFSSLSRSTG VFLTPANFLY YNLDPQNLAR HGTHARLTHL AVNGFLLFGV
LHAQALQAAW QQLHACLRAS PQTGLSRVRG ARGLLSSSKS YLLLFYFTPL LLLSAFSHQE
ARFLIPLLVP LVLLCSPQTQ PIPWKGTLVL FNILGALIFG CLHQGGLVPG LKYLEQIVHT
PDLSGTTTHY TLLFTHTYMP PQHLLHLSGL GSPVEVVDMG GAEDRVLCQA LNNFSRQPAC
QLAGEPWPCR LFVVTPGTNR HALEKCRFPL KNETLLFPHL TLEDPPALSS LLSGAWRKHL
SLHVIELETP VVTKQPKAQP
