PIK1_ORYSJ
ID PIK1_ORYSJ Reviewed; 1143 AA.
AC F2VYU4; D5L9G3;
DT 18-JUL-2018, integrated into UniProtKB/Swiss-Prot.
DT 31-MAY-2011, sequence version 1.
DT 25-MAY-2022, entry version 39.
DE RecName: Full=Disease resistance protein Pik-1 {ECO:0000305};
DE AltName: Full=Pik-1 blast resistance protein {ECO:0000312|EMBL:ADZ48537.1};
GN Name=PIK-1 {ECO:0000312|EMBL:ADZ48537.1};
GN Synonyms=PIK1-KA {ECO:0000312|EMBL:BAL63004.1,
GN ECO:0000312|EMBL:BAL63005.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC STRAIN=cv. Kusabue;
RX PubMed=21118257; DOI=10.1111/j.1469-8137.2010.03462.x;
RA Zhai C., Lin F., Dong Z., He X., Yuan B., Zeng X., Wang L., Pan Q.;
RT "The isolation and characterization of Pik, a rice blast resistance gene
RT which emerged after rice domestication.";
RL New Phytol. 189:321-334(2011).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], AND FUNCTION.
RC STRAIN=cv. Kanto 51;
RX DOI=10.1007/s11032-011-9638-y;
RA Ashikawa I., Hayashi N., Abe F., Wu J., Matsumoto T.;
RT "Characterization of the rice blast resistance gene Pik cloned from
RT Kanto51.";
RL Mol. Breed. 30:485-494(2012).
RN [3]
RP FUNCTION, AND INTERACTION WITH AVR-PIK.
RC STRAIN=cv. Kanto 51;
RX PubMed=22805093; DOI=10.1111/j.1365-313x.2012.05110.x;
RA Kanzaki H., Yoshida K., Saitoh H., Fujisaki K., Hirabuchi A., Alaux L.,
RA Fournier E., Tharreau D., Terauchi R.;
RT "Arms race co-evolution of Magnaporthe oryzae AVR-Pik and rice Pik genes
RT driven by their physical interactions.";
RL Plant J. 72:894-907(2012).
RN [4]
RP FUNCTION, INTERACTION WITH AVR-PIK, AND DOMAIN.
RX PubMed=23548743; DOI=10.1105/tpc.112.107201;
RA Cesari S., Thilliez G., Ribot C., Chalvon V., Michel C., Jauneau A.,
RA Rivas S., Alaux L., Kanzaki H., Okuyama Y., Morel J.B., Fournier E.,
RA Tharreau D., Terauchi R., Kroj T.;
RT "The rice resistance protein pair RGA4/RGA5 recognizes the Magnaporthe
RT oryzae effectors AVR-Pia and AVR1-CO39 by direct binding.";
RL Plant Cell 25:1463-1481(2013).
CC -!- FUNCTION: Disease resistance (R) protein that specifically recognizes
CC the AVR-Pik effector avirulence protein from M.oryzae. Resistance
CC proteins guard the plant against pathogens that contain an appropriate
CC avirulence protein via an indirect interaction with this avirulence
CC protein. That triggers a defense system including the hypersensitive
CC response, which restricts the pathogen growth. Contribution of Pik-2 is
CC required to recognize the effector avirulence protein AVR-Pik.
CC {ECO:0000269|PubMed:21118257, ECO:0000269|PubMed:22805093,
CC ECO:0000269|PubMed:23548743, ECO:0000269|Ref.2}.
CC -!- SUBUNIT: Interacts with AVR-Pik through its N-terminal part containing
CC the HMA-like domain. {ECO:0000269|PubMed:22805093,
CC ECO:0000269|PubMed:23548743}.
CC -!- DOMAIN: The HMA-like (RATX1) domain is responsible for the specific
CC recognition of AVR effectors. {ECO:0000269|PubMed:23548743}.
CC -!- SIMILARITY: Belongs to the disease resistance NB-LRR family.
CC {ECO:0000305}.
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DR EMBL; HM048900; ADZ48537.1; -; Genomic_DNA.
DR EMBL; AB616658; BAL63004.1; -; mRNA.
DR EMBL; AB616659; BAL63005.1; -; Genomic_DNA.
DR AlphaFoldDB; F2VYU4; -.
DR SMR; F2VYU4; -.
DR GO; GO:0043531; F:ADP binding; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0042742; P:defense response to bacterium; IMP:UniProtKB.
DR GO; GO:0002758; P:innate immune response-activating signal transduction; IMP:UniProtKB.
DR GO; GO:0009626; P:plant-type hypersensitive response; IMP:UniProtKB.
DR Gene3D; 1.10.10.10; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR Gene3D; 3.80.10.10; -; 1.
DR InterPro; IPR044974; Disease_R_plants.
DR InterPro; IPR003591; Leu-rich_rpt_typical-subtyp.
DR InterPro; IPR032675; LRR_dom_sf.
DR InterPro; IPR002182; NB-ARC.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR041118; Rx_N.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR PANTHER; PTHR23155; PTHR23155; 2.
DR Pfam; PF00931; NB-ARC; 1.
DR Pfam; PF18052; Rx_N; 1.
DR SMART; SM00369; LRR_TYP; 3.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS50846; HMA_2; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Coiled coil; Leucine-rich repeat; Nucleotide-binding;
KW Plant defense; Repeat.
FT CHAIN 1..1143
FT /note="Disease resistance protein Pik-1"
FT /id="PRO_0000444663"
FT DOMAIN 189..258
FT /note="HMA"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00280"
FT DOMAIN 282..570
FT /note="NB-ARC"
FT /evidence="ECO:0000255"
FT REPEAT 681..706
FT /note="LRR 1"
FT /evidence="ECO:0000255"
FT REPEAT 708..731
FT /note="LRR 2"
FT /evidence="ECO:0000255"
FT REPEAT 732..754
FT /note="LRR 3"
FT /evidence="ECO:0000255"
FT REPEAT 756..777
FT /note="LRR 4"
FT /evidence="ECO:0000255"
FT REPEAT 778..800
FT /note="LRR 5"
FT /evidence="ECO:0000255"
FT REPEAT 802..823
FT /note="LRR 6"
FT /evidence="ECO:0000255"
FT REPEAT 824..848
FT /note="LRR 7"
FT /evidence="ECO:0000255"
FT REPEAT 945..968
FT /note="LRR 8"
FT /evidence="ECO:0000255"
FT REPEAT 979..1002
FT /note="LRR 9"
FT /evidence="ECO:0000255"
FT REPEAT 1004..1027
FT /note="LRR 10"
FT /evidence="ECO:0000255"
FT REGION 1..190
FT /note="Structured coiled coil (CC) domain"
FT /evidence="ECO:0000303|PubMed:21118257"
FT REGION 191..264
FT /note="HMA-like domain"
FT /evidence="ECO:0000269|PubMed:23548743"
FT CONFLICT 465
FT /note="S -> P (in Ref. 2; BAL63004/BAL63005)"
FT /evidence="ECO:0000305"
FT CONFLICT 495
FT /note="G -> R (in Ref. 2; BAL63004/BAL63005)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1143 AA; 126799 MW; 3CD263EFA3BF5966 CRC64;
MEAAAMAVTA ATGALAPVLV KLAALLDDGE CNLLEGSRSD AEFIRSELEA VHSLLTPNIL
GRMGDDDAAC KDGLIAEVRE LSYDLDDAVD DFLELNFEQR RSASPFGELK ARVEERVSNR
FSDWKLPAAS LPPSSVHRRA GLPPPDAGLV GMHKRKEELI ELLEQGSSDA SRWRKRKPHV
PLRIMGGEMQ KIVFKIPMVD DKSRTKAMSL VASTVGVHSV AIAGDLRDEV VVVGDGIDSI
NLVSALRKKV GHAELLQVSQ VKEDVKEITA MLAPVKSICE FHEVKTICIL GLPGGGKTTI
ARVLYHALGT QFQCRVFASI SPSSSPSPNL TETLADIFAQ AQLGVTDTLS TPYGGSGTGR
ALQQHLIDNI SAFLLNKKYL IVIDDIWHWE EWEVIRKSIP KNDLGGRIIM TTRLNSIAEK
CHTDDNDVFV YEVGDLDNND ALSLSWGIAT KSGAGNRIGT GEDNSCYDIV NMCYGMPLAL
IWLSSALVGE IEELGGAEVK KCRDLRHIED GILDIPSLQP LAESLCLGYN HLPLYLRTLL
LYCSAYHWSN RIERGRLVRR WIAEGFVSEE KEAEGYFGEL INRGWITQHG DNNSYNYYEI
HPVMLAFLRC KSKEYNFLTC LGLGSDTSTS ASSPRLIRRL SLQGGYPVDC LSSMSMDVSH
TCSLVVLGDV ARPKGIPFYM FKRLRVLDLE DNKDIQDSHL QGICEQLSLR VRYLGLKGTR
IRKLPQEMRK LKHLEILYVG STRISELPQE IGELKHLRIL DVRNTDITEL PLQIRELQHL
HTLDVRNTPI SELPPQVGKL QNLKIMCVRS TGVRELPKEI GELNHLQTLD VRNTRVRELP
WQAGQISQSL RVLAGDSGDG VRLPEGVCEA LINGIPGATR AKCREVLSIA IIDRFGPPLV
GIFKVPGSHM RIPKMIKDHF RVLSCLDIRL CHKLEDDDQK FLAEMPNLQT LVLRFEALPR
QPITINGTGF QMLESFRVDS RLPRIAFHED AMPNLKLLEF KFYAGPASND AIGITNLKSL
QKVVFRCSPW YKSDAPGISA TIDVVKKEAE EHPNRPITLL INAGYKEIST ESHGSSENIA
GSSGIDTEPA QAQHDNLPAV RDDYKGKGIL LDGRCPTCGR ATKIEEETQD RVADIEIQTE
TTS
