ID   PIL3_ECOLX              Reviewed;          21 AA.
AC   P13948;
DT   01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1990, sequence version 1.
DT   03-AUG-2022, entry version 52.
DE   RecName: Full=Pilin;
DE   Flags: Precursor; Fragment;
GN   Name=traA;
OS   Escherichia coli.
OG   Plasmid ColB4-K98.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3531163; DOI=10.1128/jb.168.1.132-139.1986;
RA   Finlay B.B., Frost L.S., Paranchych W.;
RT   "Origin of transfer of IncF plasmids and nucleotide sequences of the type
RT   II oriT, traM, and traY alleles from ColB4-K98 and the type IV traY allele
RT   from R100-1.";
RL   J. Bacteriol. 168:132-139(1986).
CC   -!- FUNCTION: Propilin is the precursor of the pilus subunit, pilin, that
CC       forms conjugative pili, the filamentous surface appendages required for
CC       cell-to-cell contact during the earlier stages of bacterial
CC       conjugation, and that retract after contact is established. Mature
CC       pilin is assembled with the help of TraQ and TraX (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Monomer. Interacts with itself to form filaments; also
CC       interacts with TraQ (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
CC       Secreted {ECO:0000250}. Note=Propilin is directed to the inner
CC       membrane, where it is cleaved and acetylated. Mature pilin forms
CC       filaments that are secreted to form the conjugative pilus (By
CC       similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the TraA family. {ECO:0000305}.
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DR   EMBL; M15135; AAB04666.1; -; Genomic_DNA.
DR   AlphaFoldDB; P13948; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR008873; TraA.
DR   Pfam; PF05513; TraA; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Conjugation; Membrane; Plasmid;
KW   Secreted.
FT   PROPEP          1..>21
FT                   /id="PRO_0000024489"
FT   REGION          1..21
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   NON_TER         21
SQ   SEQUENCE   21 AA;  2198 MW;  076C0D0C5E9D14EA CRC64;
     MDAVLSVQGA SAPVKKKSFF S