PILC_PSEAE
ID PILC_PSEAE Reviewed; 374 AA.
AC P22609; Q9HVP9;
DT 01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT 04-MAY-2001, sequence version 2.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=Type IV pilus assembly protein PilC;
GN Name=pilC; OrderedLocusNames=PA4527;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC STRAIN=PAK;
RX PubMed=1971619; DOI=10.1128/jb.172.6.2911-2919.1990;
RA Nunn D., Bergman S., Lory S.;
RT "Products of three accessory genes, pilB, pilC, and pilD, are required for
RT biogenesis of Pseudomonas aeruginosa pili.";
RL J. Bacteriol. 172:2911-2919(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
RN [3]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=15659660; DOI=10.1128/jb.187.3.829-839.2005;
RA Chiang P., Habash M., Burrows L.L.;
RT "Disparate subcellular localization patterns of Pseudomonas aeruginosa Type
RT IV pilus ATPases involved in twitching motility.";
RL J. Bacteriol. 187:829-839(2005).
RN [4]
RP INTERACTION WITH PILB, FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=23413032; DOI=10.1074/jbc.m113.453506;
RA Takhar H.K., Kemp K., Kim M., Howell P.L., Burrows L.L.;
RT "The platform protein is essential for type IV pilus biogenesis.";
RL J. Biol. Chem. 288:9721-9728(2013).
CC -!- FUNCTION: Essential inner membrane component of the type IV pilus (T4P)
CC that plays a role in surface and host cell adhesion, colonization,
CC biofilm maturation, virulence, and twitching, a form of surface-
CC associated motility facilitated by cycles of extension, adhesion, and
CC retraction of T4P fibers. Controls both pilus assembly and disassembly
CC and plays an important role in PilB localization to the complex and
CC ATPase activity. {ECO:0000269|PubMed:15659660,
CC ECO:0000269|PubMed:1971619, ECO:0000269|PubMed:23413032}.
CC -!- SUBUNIT: Homotetramer (By similarity). Interacts with PilB
CC (PubMed:23413032). {ECO:0000250|UniProtKB:Q5SK58,
CC ECO:0000269|PubMed:23413032}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Multi-pass
CC membrane protein {ECO:0000305}.
CC -!- DISRUPTION PHENOTYPE: Deletion mutants lack surface pili showing the
CC essential role of PilC for type IV pilus (T4P) biogenesis
CC (PubMed:23413032). In addition, PilC mutants show a diffuse cytoplasmic
CC localization of PilB (PubMed:15659660). {ECO:0000269|PubMed:15659660,
CC ECO:0000269|PubMed:23413032}.
CC -!- SIMILARITY: Belongs to the GSP F family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA25733.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; M32066; AAA25733.1; ALT_INIT; Genomic_DNA.
DR EMBL; AE004091; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR PIR; B35384; B35384.
DR PIR; C83078; C83078.
DR RefSeq; WP_003148138.1; NZ_CP053028.1.
DR AlphaFoldDB; P22609; -.
DR SMR; P22609; -.
DR TCDB; 3.A.15.2.1; the outer membrane protein secreting main terminal branch (mtb) family.
DR PRIDE; P22609; -.
DR PseudoCAP; PA4527; -.
DR InParanoid; P22609; -.
DR OMA; TRQMATM; -.
DR PhylomeDB; P22609; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0015628; P:protein secretion by the type II secretion system; IBA:GO_Central.
DR Gene3D; 1.20.81.30; -; 2.
DR InterPro; IPR003004; GspF/PilC.
DR InterPro; IPR001992; T2SS_GspF/T4SS_PilC_CS.
DR InterPro; IPR018076; T2SS_GspF_dom.
DR InterPro; IPR042094; T2SS_GspF_sf.
DR PANTHER; PTHR30012; PTHR30012; 1.
DR Pfam; PF00482; T2SSF; 2.
DR PRINTS; PR00812; BCTERIALGSPF.
DR PROSITE; PS00874; T2SP_F; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Fimbrium biogenesis; Membrane;
KW Protein transport; Reference proteome; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..374
FT /note="Type IV pilus assembly protein PilC"
FT /id="PRO_0000207840"
FT TRANSMEM 138..158
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 187..207
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 347..367
FT /note="Helical"
FT /evidence="ECO:0000255"
FT VARIANT 136
FT /note="R -> K (in strain: PAK)"
FT VARIANT 169
FT /note="Q -> E (in strain: PAK)"
FT VARIANT 182
FT /note="V -> I (in strain: PAK)"
FT VARIANT 189
FT /note="L -> M (in strain: PAK)"
FT VARIANT 215
FT /note="K -> Q (in strain: PAK)"
FT CONFLICT 194..206
FT /note="LAVIVGVGAIGFT -> FFIILAIAIFGFA (in Ref. 1;
FT AE004091)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 374 AA; 40887 MW; D7677D18BF77BF79 CRC64;
MLVKAHLRKQ GINPLKVRKK GISLLGAGKK VKPMDIALFT RQMATMMGAG VPLLQSFDII
GEGFDNPNMR KLVDEIKQEV SSGNSLANSL RKKPQYFDEL YCNLVDAGEQ SGALENLLDR
VATYKEKTES LKAKIRKAMT YPIAVIIVAL IVSAILLIKV VPQFQSVFQG FGAELPAFTQ
MVVNLSEFLQ EWWLAVIVGV GAIGFTFKEL HKRSKKFRDT LDRTILKLPI FGGIVYKSAV
ARYARTLSTT FAAGVPLVDA LDSVSGATGN IVFKNAVSKI KQDVSTGMQL NFSMRTTSVF
PNMAIQMTAI GEESGSLDEM LSKVASYYEE EVDNAVDNLT TLMEPMIMAV LGVLVGGLIV
AMYLPIFQLG NVVG
