ID   PILE_PSEAE              Reviewed;         141 AA.
AC   G3XD43;
DT   17-JUN-2020, integrated into UniProtKB/Swiss-Prot.
DT   16-NOV-2011, sequence version 1.
DT   25-MAY-2022, entry version 59.
DE   RecName: Full=Type IV pilus non-core minor pilin PilE {ECO:0000303|PubMed:26359492};
DE   Flags: Precursor;
GN   Name=pilE; OrderedLocusNames=PA4556;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
RN   [2]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=7854130; DOI=10.1111/j.1365-2958.1994.tb00489.x;
RA   Russell M.A., Darzins A.;
RT   "The pilE gene product of Pseudomonas aeruginosa, required for pilus
RT   biogenesis, shares amino acid sequence identity with the N-termini of type
RT   4 prepilin proteins.";
RL   Mol. Microbiol. 13:973-985(1994).
RN   [3]
RP   FUNCTION, DISRUPTION PHENOTYPE, AND CLEAVAGE BY PILD.
RX   PubMed=20338182; DOI=10.1016/j.jmb.2010.03.028;
RA   Giltner C.L., Habash M., Burrows L.L.;
RT   "Pseudomonas aeruginosa minor pilins are incorporated into type IV pili.";
RL   J. Mol. Biol. 398:444-461(2010).
RN   [4] {ECO:0007744|PDB:4NOA}
RP   X-RAY CRYSTALLOGRAPHY (1.25 ANGSTROMS) OF 36-141, DISULFIDE BONDS,
RP   MUTAGENESIS OF CYS-139, FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=26359492; DOI=10.1074/jbc.m115.683334;
RA   Nguyen Y., Harvey H., Sugiman-Marangos S., Bell S.D., Buensuceso R.N.,
RA   Junop M.S., Burrows L.L.;
RT   "Structural and functional studies of the Pseudomonas aeruginosa minor
RT   pilin, PilE.";
RL   J. Biol. Chem. 290:26856-26865(2015).
CC   -!- FUNCTION: Essential component of the type IV pilus (T4P) that plays a
CC       role in surface and host cell adhesion, colonization, biofilm
CC       maturation, virulence, and twitching, a form of surface-associated
CC       motility facilitated by cycles of extension, adhesion, and retraction
CC       of T4P fibers (PubMed:7854130, PubMed:20338182). May stabilize
CC       interactions between the major and a minor pilin subcomplex required
CC       for efficient pilus biogenesis (PubMed:26359492).
CC       {ECO:0000269|PubMed:20338182, ECO:0000269|PubMed:26359492,
CC       ECO:0000269|PubMed:7854130}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255}; Single-pass
CC       membrane protein {ECO:0000255}.
CC   -!- PTM: Methylated by prepilin peptidase PilD at the amino group of the N-
CC       terminal phenylalanine once the leader sequence is cleaved.
CC       {ECO:0000269|PubMed:20338182}.
CC   -!- DISRUPTION PHENOTYPE: Mutants show lack of surface piliation and
CC       twitching motility (PubMed:26359492, PubMed:7854130). Although
CC       piliation is abolished, PilA stability is not affected
CC       (PubMed:20338182). {ECO:0000269|PubMed:20338182,
CC       ECO:0000269|PubMed:26359492, ECO:0000269|PubMed:7854130}.
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DR   EMBL; AE004091; AAG07944.1; -; Genomic_DNA.
DR   PIR; S54700; S54700.
DR   RefSeq; NP_253246.1; NC_002516.2.
DR   RefSeq; WP_003094721.1; NZ_QZGE01000004.1.
DR   PDB; 4NOA; X-ray; 1.25 A; A=36-141.
DR   PDBsum; 4NOA; -.
DR   AlphaFoldDB; G3XD43; -.
DR   SMR; G3XD43; -.
DR   STRING; 287.DR97_1860; -.
DR   PaxDb; G3XD43; -.
DR   DNASU; 878451; -.
DR   EnsemblBacteria; AAG07944; AAG07944; PA4556.
DR   GeneID; 878451; -.
DR   KEGG; pae:PA4556; -.
DR   PATRIC; fig|208964.12.peg.4768; -.
DR   PseudoCAP; PA4556; -.
DR   HOGENOM; CLU_091705_6_0_6; -.
DR   InParanoid; G3XD43; -.
DR   OMA; IPSYREH; -.
DR   PhylomeDB; G3XD43; -.
DR   Proteomes; UP000002438; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0009297; P:pilus assembly; IDA:PseudoCAP.
DR   GO; GO:0043683; P:type IV pilus assembly; IMP:PseudoCAP.
DR   GO; GO:0043107; P:type IV pilus-dependent motility; IMP:PseudoCAP.
DR   InterPro; IPR012902; N_methyl_site.
DR   InterPro; IPR031982; PilE-like.
DR   InterPro; IPR045584; Pilin-like.
DR   Pfam; PF16732; ComP_DUS; 1.
DR   Pfam; PF07963; N_methyl; 1.
DR   SUPFAM; SSF54523; SSF54523; 1.
DR   TIGRFAMs; TIGR02532; IV_pilin_GFxxxE; 1.
DR   PROSITE; PS00409; PROKAR_NTER_METHYL; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cell inner membrane; Cell membrane; Disulfide bond; Membrane;
KW   Reference proteome; Transmembrane; Transmembrane helix.
FT   PROPEP          1..7
FT                   /note="Leader sequence"
FT                   /evidence="ECO:0000269|PubMed:20338182"
FT                   /id="PRO_0000450350"
FT   CHAIN           8..141
FT                   /note="Type IV pilus non-core minor pilin PilE"
FT                   /id="PRO_0000450351"
FT   TRANSMEM        8..28
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DISULFID        113..139
FT                   /evidence="ECO:0000269|PubMed:26359492,
FT                   ECO:0007744|PDB:4NOA"
FT   MUTAGEN         139
FT                   /note="C->A: Loss of pilus assembly and twitching
FT                   motility."
FT                   /evidence="ECO:0000269|PubMed:26359492"
FT   HELIX           36..60
FT                   /evidence="ECO:0007829|PDB:4NOA"
FT   TURN            62..64
FT                   /evidence="ECO:0007829|PDB:4NOA"
FT   HELIX           70..73
FT                   /evidence="ECO:0007829|PDB:4NOA"
FT   STRAND          83..90
FT                   /evidence="ECO:0007829|PDB:4NOA"
FT   STRAND          98..103
FT                   /evidence="ECO:0007829|PDB:4NOA"
FT   HELIX           105..109
FT                   /evidence="ECO:0007829|PDB:4NOA"
FT   TURN            111..113
FT                   /evidence="ECO:0007829|PDB:4NOA"
FT   STRAND          115..119
FT                   /evidence="ECO:0007829|PDB:4NOA"
FT   STRAND          124..126
FT                   /evidence="ECO:0007829|PDB:4NOA"
FT   STRAND          129..133
FT                   /evidence="ECO:0007829|PDB:4NOA"
FT   HELIX           134..139
FT                   /evidence="ECO:0007829|PDB:4NOA"
SQ   SEQUENCE   141 AA;  15279 MW;  9CC4D2605B76D294 CRC64;
     MRTRQKGFTL LEMVVVVAVI GILLGIAIPS YQNYVIRSNR TEGQALLSDA AARQERYYSQ
     NPGVGYTKDV AKLGMSSANS PNNLYNLTIA TPTSTTYTLT ATPINSQTRD KTCGKLTLNQ
     LGERGAAGKT GNNSTVNDCW R