ID   PILF_NEIGO              Reviewed;         558 AA.
AC   P37094;
DT   01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1994, sequence version 1.
DT   03-AUG-2022, entry version 84.
DE   RecName: Full=Type IV pilus assembly protein PilF;
GN   Name=pilF;
OS   Neisseria gonorrhoeae.
OC   Bacteria; Proteobacteria; Betaproteobacteria; Neisseriales; Neisseriaceae;
OC   Neisseria.
OX   NCBI_TaxID=485;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=MS11;
RX   PubMed=8100347; DOI=10.1111/j.1365-2958.1993.tb01579.x;
RA   Lauer P., Albertson N.H., Koomey M.;
RT   "Conservation of genes encoding components of a type IV pilus assembly/two-
RT   step protein export pathway in Neisseria gonorrhoeae.";
RL   Mol. Microbiol. 8:357-368(1993).
CC   -!- FUNCTION: Involved in the translocation of the type IV pilin.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the GSP E family. {ECO:0000305}.
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DR   EMBL; U32588; AAC43465.1; -; Genomic_DNA.
DR   PIR; S32914; S32914.
DR   AlphaFoldDB; P37094; -.
DR   SMR; P37094; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR   GO; GO:0009297; P:pilus assembly; IEA:InterPro.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.300.160; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR013374; ATPase_typ4_pilus-assembl_PilB.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR001482; T2SS/T4SS.
DR   InterPro; IPR037257; T2SS_E_N_sf.
DR   InterPro; IPR007831; T2SS_GspE_N.
DR   Pfam; PF00437; T2SSE; 1.
DR   Pfam; PF05157; T2SSE_N; 1.
DR   SMART; SM00382; AAA; 1.
DR   SUPFAM; SSF160246; SSF160246; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR02538; type_IV_pilB; 1.
DR   PROSITE; PS00662; T2SP_E; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Cytoplasm; Fimbrium biogenesis; Nucleotide-binding;
KW   Protein transport; Transport.
FT   CHAIN           1..558
FT                   /note="Type IV pilus assembly protein PilF"
FT                   /id="PRO_0000207297"
FT   BINDING         315..322
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   558 AA;  61764 MW;  5241AE2EFC44D0C8 CRC64;
     MSVGLLRILV QNQVVTVEQA EHYYNESQAG KEVLPMLFSD GVISPKSLAA LIARVFSYSI
     LDLRHYPRHR VLMGVLTEEQ MVEFHCVPVF RRGDKVFFAV SDPTQMPQIQ KTVSAAGIAV
     ELVIVEDDQL AGLLDWVGSR STSLLQELGE GQEEEESHTL YIDNEEAEDG PVPRFIHKTL
     SDALRSGASD IHFEFYEHNA RIRFRVDGQL REVVQPPIAV RGQLASRIKV MSRLDISEKR
     IPQDGRMQLT FQKGGKPVDF RVSTLPTLFG EKVVMRILNS DAASLNIDQL GFEPFQKKLL
     LEAIHRPYGM VLVTGPTGSG KTVSLYTCLN ILNTESVNIA TAEDPAEINL PGINQVNVND
     KQGLTFAAAL KSFLRQDPDI IMVGEIRDLE TADIAIKAAQ TGHMVFSTLH TNNAPATLSR
     MLNMGVAPFN IASSVSLIMA QRLLRRLCSS CKQEVERPSA SALKEVGFTD EDLAKDWKLY
     GAVGCDRCRG QGYKGRAGVY EVMPISEEMQ RVIMNNGTEV GILDVAYKEG MVDLRRAGIL
     KIMQGITSLE EVTANTND