ID   PILN_PSEAE              Reviewed;         198 AA.
AC   G3XD30;
DT   12-AUG-2020, integrated into UniProtKB/Swiss-Prot.
DT   16-NOV-2011, sequence version 1.
DT   03-AUG-2022, entry version 52.
DE   RecName: Full=Type IV pilus inner membrane component PilN {ECO:0000303|PubMed:19857645};
GN   Name=pilN; OrderedLocusNames=PA5043;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
RN   [2]
RP   FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH PILM; PILO AND PILP, AND
RP   DISRUPTION PHENOTYPE.
RX   PubMed=19857645; DOI=10.1016/j.jmb.2009.09.034;
RA   Ayers M., Sampaleanu L.M., Tammam S., Koo J., Harvey H., Howell P.L.,
RA   Burrows L.L.;
RT   "PilM/N/O/P proteins form an inner membrane complex that affects the
RT   stability of the Pseudomonas aeruginosa type IV pilus secretin.";
RL   J. Mol. Biol. 394:128-142(2009).
RN   [3]
RP   FUNCTION, AND INTERACTION WITH PILO AND PILM.
RX   PubMed=19857646; DOI=10.1016/j.jmb.2009.09.037;
RA   Sampaleanu L.M., Bonanno J.B., Ayers M., Koo J., Tammam S., Burley S.K.,
RA   Almo S.C., Burrows L.L., Howell P.L.;
RT   "Periplasmic domains of Pseudomonas aeruginosa PilN and PilO form a stable
RT   heterodimeric complex.";
RL   J. Mol. Biol. 394:143-159(2009).
RN   [4]
RP   INTERACTION WITH PILP AND PILO.
RX   PubMed=22053789; DOI=10.1111/j.1365-2958.2011.07903.x;
RA   Tammam S., Sampaleanu L.M., Koo J., Sundaram P., Ayers M., Chong P.A.,
RA   Forman-Kay J.D., Burrows L.L., Howell P.L.;
RT   "Characterization of the PilN, PilO and PilP type IVa pilus subcomplex.";
RL   Mol. Microbiol. 82:1496-1514(2011).
RN   [5]
RP   INTERACTION WITH PILO, AND SUBUNIT.
RX   PubMed=27474743; DOI=10.1074/jbc.m116.738377;
RA   Leighton T.L., Yong D.H., Howell P.L., Burrows L.L.;
RT   "Type IV Pilus Alignment Subcomplex Proteins PilN and PilO Form Homo- and
RT   Heterodimers in Vivo.";
RL   J. Biol. Chem. 291:19923-19938(2016).
RN   [6] {ECO:0007744|PDB:5EOU}
RP   X-RAY CRYSTALLOGRAPHY (2.40 ANGSTROMS) OF 1-12, FUNCTION, AND INTERACTION
RP   WITH PILM.
RX   PubMed=27022027; DOI=10.1074/jbc.m116.718353;
RA   McCallum M., Tammam S., Little D.J., Robinson H., Koo J., Shah M.,
RA   Calmettes C., Moraes T.F., Burrows L.L., Howell P.L.;
RT   "PilN Binding Modulates the Structure and Binding Partners of the
RT   Pseudomonas aeruginosa Type IVa Pilus Protein PilM.";
RL   J. Biol. Chem. 291:11003-11015(2016).
CC   -!- FUNCTION: Inner membrane component of the type IV (T4S) secretion
CC       system that plays a role in surface and host cell adhesion,
CC       colonization, biofilm maturation, virulence, and twitching, a form of
CC       surface-associated motility. PilN/PilO heterodimers form the foundation
CC       of the inner-membrane PilM/PilN/PilO/PilP complex which plays an
CC       essential role in the assembly of a functional T4 pilus
CC       (PubMed:19857645, PubMed:19857646). In turn, associates with PilM and
CC       facilitates PilM functionally relevant structural changes
CC       (PubMed:27022027). {ECO:0000269|PubMed:19857645,
CC       ECO:0000269|PubMed:19857646, ECO:0000269|PubMed:27022027}.
CC   -!- SUBUNIT: Homodimer (PubMed:27474743). Interacts with PilO
CC       (PubMed:19857645, PubMed:19857646, PubMed:22053789, PubMed:27474743).
CC       Interacts with PilP (via N-terminus) (PubMed:22053789). Interacts (via
CC       N-terminus) with PilM; this interaction modulates PilM ATP binding site
CC       (PubMed:19857646, PubMed:27022027). {ECO:0000269|PubMed:19857645,
CC       ECO:0000269|PubMed:19857646, ECO:0000269|PubMed:22053789,
CC       ECO:0000269|PubMed:27022027, ECO:0000269|PubMed:27474743}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane
CC       {ECO:0000269|PubMed:19857645}; Single-pass type II membrane protein
CC       {ECO:0000255}.
CC   -!- DISRUPTION PHENOTYPE: Deletion mutants lack surface pili.
CC       {ECO:0000269|PubMed:19857645}.
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DR   EMBL; AE004091; AAG08428.1; -; Genomic_DNA.
DR   PIR; S77727; S77727.
DR   RefSeq; NP_253730.1; NC_002516.2.
DR   RefSeq; WP_003095839.1; NZ_QZGE01000002.1.
DR   PDB; 5EOU; X-ray; 2.40 A; A/B=1-12.
DR   PDBsum; 5EOU; -.
DR   AlphaFoldDB; G3XD30; -.
DR   SMR; G3XD30; -.
DR   STRING; 287.DR97_2398; -.
DR   PaxDb; G3XD30; -.
DR   PRIDE; G3XD30; -.
DR   EnsemblBacteria; AAG08428; AAG08428; PA5043.
DR   GeneID; 881025; -.
DR   KEGG; pae:PA5043; -.
DR   PATRIC; fig|208964.12.peg.5287; -.
DR   PseudoCAP; PA5043; -.
DR   HOGENOM; CLU_081304_1_2_6; -.
DR   InParanoid; G3XD30; -.
DR   OMA; DRNLPVH; -.
DR   PhylomeDB; G3XD30; -.
DR   Proteomes; UP000002438; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0044096; C:type IV pilus; IMP:PseudoCAP.
DR   GO; GO:0043683; P:type IV pilus assembly; IMP:PseudoCAP.
DR   GO; GO:0043107; P:type IV pilus-dependent motility; IMP:PseudoCAP.
DR   InterPro; IPR007813; PilN.
DR   Pfam; PF05137; PilN; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cell inner membrane; Cell membrane; Membrane;
KW   Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..198
FT                   /note="Type IV pilus inner membrane component PilN"
FT                   /id="PRO_0000450557"
FT   TRANSMEM        19..39
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   198 AA;  22214 MW;  7920C66DB3351DDC CRC64;
     MARINLLPWR EELREQRKQQ FLVILGGVLV ASAALVFLGD QYFTAAIENQ NARNDFLRKE
     IVVLDARIKE ISELKSRRQQ LLERMKIIQD LQGNRPIIGR VFDQLVRTLP DGVYFTDLKM
     TGKNIAIAGA AESNNRVSNL MRNMDASEWL TAPTLNEVKA VTQGAVDQAN VFQLTVQQTQ
     PGEEDAKAKH GVAQGAKK