ID   PILQ_NEIMB              Reviewed;         769 AA.
AC   Q70M91; Q4W562;
DT   29-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   29-MAR-2005, sequence version 2.
DT   25-MAY-2022, entry version 91.
DE   RecName: Full=Type IV pilus biogenesis and competence protein PilQ;
DE   Flags: Precursor;
GN   Name=pilQ; OrderedLocusNames=NMB1812;
OS   Neisseria meningitidis serogroup B (strain MC58).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Neisseriales; Neisseriaceae;
OC   Neisseria.
OX   NCBI_TaxID=122586;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND STRUCTURE BY ELECTRON MICROSCOPY (26
RP   ANGSTROMS).
RC   STRAIN=CCUG 37602 / M1080 / Serogroup B / Serotype 1;
RX   PubMed=15254043; DOI=10.1074/jbc.m405971200;
RA   Collins R.F., Frye S.A., Kitmitto A., Ford R.C., Toenjum T., Derrick J.P.;
RT   "Structure of the Neisseria meningitidis outer membrane PilQ secretin
RT   complex at 12 A resolution.";
RL   J. Biol. Chem. 279:39750-39756(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MC58;
RX   PubMed=10710307; DOI=10.1126/science.287.5459.1809;
RA   Tettelin H., Saunders N.J., Heidelberg J.F., Jeffries A.C., Nelson K.E.,
RA   Eisen J.A., Ketchum K.A., Hood D.W., Peden J.F., Dodson R.J., Nelson W.C.,
RA   Gwinn M.L., DeBoy R.T., Peterson J.D., Hickey E.K., Haft D.H.,
RA   Salzberg S.L., White O., Fleischmann R.D., Dougherty B.A., Mason T.M.,
RA   Ciecko A., Parksey D.S., Blair E., Cittone H., Clark E.B., Cotton M.D.,
RA   Utterback T.R., Khouri H.M., Qin H., Vamathevan J.J., Gill J., Scarlato V.,
RA   Masignani V., Pizza M., Grandi G., Sun L., Smith H.O., Fraser C.M.,
RA   Moxon E.R., Rappuoli R., Venter J.C.;
RT   "Complete genome sequence of Neisseria meningitidis serogroup B strain
RT   MC58.";
RL   Science 287:1809-1815(2000).
RN   [3]
RP   SUBUNIT.
RC   STRAIN=CCUG 37602 / M1080 / Serogroup B / Serotype 1;
RX   PubMed=11395444; DOI=10.1128/jb.183.13.3825-3832.2001;
RA   Collins R.F., Davidsen L., Derrick J.P., Ford R.C., Toenjum T.;
RT   "Analysis of the PilQ secretin from Neisseria meningitidis by transmission
RT   electron microscopy reveals a dodecameric quaternary structure.";
RL   J. Bacteriol. 183:3825-3832(2001).
RN   [4]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=17038831; DOI=10.4161/hv.1.2.1651;
RA   Vipond C., Wheeler J.X., Jones C., Feavers I.M., Suker J.;
RT   "Characterization of the protein content of a meningococcal outer membrane
RT   vesicle vaccine by polyacrylamide gel electrophoresis and mass
RT   spectrometry.";
RL   Hum. Vaccin. 1:80-84(2005).
CC   -!- FUNCTION: Required for type IV pilus biogenesis and competence. Could
CC       function as a pore for exit of the pilus but also as a channel for
CC       entry of heme and antimicrobial agents and uptake of transforming DNA
CC       (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Homododecamer. Tetramer of trimer.
CC       {ECO:0000269|PubMed:11395444}.
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane.
CC   -!- MISCELLANEOUS: Present in outer membrane vesicle formulations which are
CC       used as vaccines in human.
CC   -!- SIMILARITY: Belongs to the bacterial secretin family. PilQ subfamily.
CC       {ECO:0000305}.
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DR   EMBL; AJ564200; CAD91899.1; -; Genomic_DNA.
DR   EMBL; AE002098; AAY52170.1; -; Genomic_DNA.
DR   RefSeq; NP_274809.1; NC_003112.2.
DR   RefSeq; WP_002225652.1; NC_003112.2.
DR   PDB; 4AQZ; NMR; -; A=224-329.
DR   PDB; 4AV2; EM; 26.00 A; A/B/C/D/E/F/G/H/I/J/K/L=25-769.
DR   PDBsum; 4AQZ; -.
DR   PDBsum; 4AV2; -.
DR   AlphaFoldDB; Q70M91; -.
DR   BMRB; Q70M91; -.
DR   SMR; Q70M91; -.
DR   IntAct; Q70M91; 1.
DR   STRING; 122586.NMB1812; -.
DR   PaxDb; Q70M91; -.
DR   EnsemblBacteria; AAY52170; AAY52170; NMB1812.
DR   KEGG; nme:NMB1812; -.
DR   PATRIC; fig|122586.8.peg.2302; -.
DR   HOGENOM; CLU_006756_0_1_4; -.
DR   OMA; QNVPWDQ; -.
DR   PHI-base; PHI:3827; -.
DR   Proteomes; UP000000425; Chromosome.
DR   GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0030420; P:establishment of competence for transformation; IEA:UniProtKB-KW.
DR   GO; GO:0009306; P:protein secretion; IEA:InterPro.
DR   Gene3D; 3.30.1370.120; -; 1.
DR   InterPro; IPR021731; AMIN_dom.
DR   InterPro; IPR001775; GspD/PilQ.
DR   InterPro; IPR005644; NolW-like.
DR   InterPro; IPR038591; NolW-like_sf.
DR   InterPro; IPR013355; Pilus_4_PilQ.
DR   InterPro; IPR011662; Secretin/TonB_short_N.
DR   InterPro; IPR004846; T2SS/T3SS.
DR   InterPro; IPR004845; T2SS_GspD_CS.
DR   Pfam; PF11741; AMIN; 1.
DR   Pfam; PF00263; Secretin; 1.
DR   Pfam; PF03958; Secretin_N; 1.
DR   Pfam; PF07660; STN; 1.
DR   PRINTS; PR00811; BCTERIALGSPD.
DR   SMART; SM00965; STN; 1.
DR   TIGRFAMs; TIGR02515; IV_pilus_PilQ; 1.
DR   PROSITE; PS00875; T2SP_D; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cell outer membrane; Competence; Membrane; Protein transport;
KW   Reference proteome; Signal; Transport.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000255"
FT   CHAIN           25..769
FT                   /note="Type IV pilus biogenesis and competence protein
FT                   PilQ"
FT                   /id="PRO_0000013117"
FT   REGION          135..156
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          197..228
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   VARIANT         203
FT                   /note="A -> T (in strain: CCUG 37602)"
FT   VARIANT         218..225
FT                   /note="Missing (in strain: CCUG 37602)"
FT   VARIANT         428
FT                   /note="L -> F (in strain: CCUG 37602)"
FT   VARIANT         461
FT                   /note="R -> A (in strain: CCUG 37602)"
FT   VARIANT         475
FT                   /note="I -> V (in strain: CCUG 37602)"
FT   VARIANT         607
FT                   /note="T -> P (in strain: CCUG 37602)"
FT   STRAND          227..233
FT                   /evidence="ECO:0007829|PDB:4AQZ"
FT   TURN            234..236
FT                   /evidence="ECO:0007829|PDB:4AQZ"
FT   STRAND          237..243
FT                   /evidence="ECO:0007829|PDB:4AQZ"
FT   STRAND          252..255
FT                   /evidence="ECO:0007829|PDB:4AQZ"
FT   STRAND          257..264
FT                   /evidence="ECO:0007829|PDB:4AQZ"
FT   TURN            271..273
FT                   /evidence="ECO:0007829|PDB:4AQZ"
FT   STRAND          282..293
FT                   /evidence="ECO:0007829|PDB:4AQZ"
FT   STRAND          295..304
FT                   /evidence="ECO:0007829|PDB:4AQZ"
FT   STRAND          306..316
FT                   /evidence="ECO:0007829|PDB:4AQZ"
FT   STRAND          319..326
FT                   /evidence="ECO:0007829|PDB:4AQZ"
SQ   SEQUENCE   769 AA;  82473 MW;  1CC24EF102DC4BC9 CRC64;
     MNTKLTKIIS GLFVATAAFQ TASAGNITDI KVSSLPNKQK IVKVSFDKEI VNPTGFVTSS
     PARIALDFEQ TGISMDQQVL EYADPLLSKI SAAQNSSRAR LVLNLNKPGQ YNTEVRGNKV
     WIFINESDDT VSAPARPAVK AAPAAPAKQQ AAAPSTKSAV SVSEPFTPAK QQAAAPFTES
     VVSVSAPFSP AKQQAAASAK QQAAAPAKQQ AAAPAKQQAA APAKQTNIDF RKDGKNAGII
     ELAALGFAGQ PDISQQHDHI IVTLKNHTLP TTLQRSLDVA DFKTPVQKVT LKRLNNDTQL
     IITTAGNWEL VNKSAAPGYF TFQVLPKKQN LESGGVNNAP KTFTGRKISL DFQDVEIRTI
     LQILAKESGM NIVASDSVNG KMTLSLKDVP WDQALDLVMQ ARNLDMRQQG NIVNIAPRDE
     LLAKDKALLQ AEKDIADLGA LYSQNFQLKY KNVEEFRSIL RLDNADTTGN RNTLISGRGS
     VLIDPATNTL IVTDTRSVIE KFRKLIDELD VPAQQVMIEA RIVEAADGFS RDLGVKFGAT
     GKKKLKNDTS AFGWGVNSGF GGDDKWGAET KINLPITAAA NSISLVRAIS SGALNLELSA
     SESLSKTKTL ANPRVLTQNR KEAKIESGYE IPFTVTSIAN GGSSTNTELK KAVLGLTVTP
     NITPDGQIIM TVKINKDSPA QCASGNQTIL CISTKNLNTQ AMVENGGTLI VGGIYEEDNG
     NTLTKVPLLG DIPVIGNLFK TRGKKTDRRE LLIFITPRIM GTAGNSLRY