PILS1_ARATH
ID PILS1_ARATH Reviewed; 472 AA.
AC F4HWB6; Q9SYP5;
DT 08-JUN-2016, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 62.
DE RecName: Full=Protein PIN-LIKES 1 {ECO:0000303|PubMed:22504182};
DE AltName: Full=Auxin efflux carrier-like protein 1 {ECO:0000303|PubMed:22504182};
GN Name=PILS1 {ECO:0000303|PubMed:22504182};
GN OrderedLocusNames=At1g20925 {ECO:0000312|Araport:AT1G20925};
GN ORFNames=F9H16.9 {ECO:0000312|EMBL:AAD30600.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|Proteomes:UP000006548};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE, AND SUBCELLULAR LOCATION.
RX PubMed=22504182; DOI=10.1038/nature11001;
RA Barbez E., Kubes M., Rolcik J., Beziat C., Pencik A., Wang B.,
RA Rosquete M.R., Zhu J., Dobrev P.I., Lee Y., Zazimalova E., Petrasek J.,
RA Geisler M., Friml J., Kleine-Vehn J.;
RT "A novel putative auxin carrier family regulates intracellular auxin
RT homeostasis in plants.";
RL Nature 485:119-122(2012).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=23091477; DOI=10.3389/fpls.2012.00227;
RA Feraru E., Vosolsobe S., Feraru M.I., Petrasek J., Kleine-Vehn J.;
RT "Evolution and structural diversification of PILS putative auxin carriers
RT in plants.";
RL Front. Plant Sci. 3:227-227(2012).
CC -!- FUNCTION: Involved in cellular auxin homeostasis by regulating auxin
CC metabolism. Regulates intracellular auxin accumulation at the
CC endoplasmic reticulum and thus auxin availability for nuclear auxin
CC signaling. {ECO:0000305|PubMed:22504182}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000269|PubMed:22504182}; Multi-pass membrane protein
CC {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in flowers.
CC {ECO:0000269|PubMed:22504182}.
CC -!- SIMILARITY: Belongs to the auxin efflux carrier (TC 2.A.69.2) family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD30600.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC007369; AAD30600.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE30042.1; -; Genomic_DNA.
DR PIR; A86342; A86342.
DR RefSeq; NP_683316.2; NM_148475.3.
DR AlphaFoldDB; F4HWB6; -.
DR STRING; 3702.AT1G20925.1; -.
DR PaxDb; F4HWB6; -.
DR PRIDE; F4HWB6; -.
DR ProteomicsDB; 235016; -.
DR EnsemblPlants; AT1G20925.1; AT1G20925.1; AT1G20925.
DR GeneID; 838686; -.
DR Gramene; AT1G20925.1; AT1G20925.1; AT1G20925.
DR KEGG; ath:AT1G20925; -.
DR Araport; AT1G20925; -.
DR TAIR; locus:504956196; AT1G20925.
DR eggNOG; KOG2722; Eukaryota.
DR HOGENOM; CLU_044945_0_0_1; -.
DR InParanoid; F4HWB6; -.
DR OMA; WLVIKIT; -.
DR OrthoDB; 1055578at2759; -.
DR PRO; PR:F4HWB6; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; F4HWB6; baseline and differential.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IDA:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0009734; P:auxin-activated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0080162; P:endoplasmic reticulum to cytosol auxin transport; IEA:InterPro.
DR InterPro; IPR004776; Mem_trans.
DR InterPro; IPR045033; PILS1/3/4/5/7.
DR PANTHER; PTHR31651; PTHR31651; 1.
DR Pfam; PF03547; Mem_trans; 2.
PE 2: Evidence at transcript level;
KW Auxin signaling pathway; Endoplasmic reticulum; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..472
FT /note="Protein PIN-LIKES 1"
FT /id="PRO_0000436496"
FT TOPO_DOM 1..93
FT /note="Lumenal"
FT /evidence="ECO:0000305|PubMed:23091477"
FT TRANSMEM 94..114
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 115..133
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305|PubMed:23091477"
FT TRANSMEM 134..154
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 155..161
FT /note="Lumenal"
FT /evidence="ECO:0000305|PubMed:23091477"
FT TRANSMEM 162..182
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 183..193
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305|PubMed:23091477"
FT TRANSMEM 194..214
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 215..231
FT /note="Lumenal"
FT /evidence="ECO:0000305|PubMed:23091477"
FT TRANSMEM 232..252
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 253..309
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305|PubMed:23091477"
FT TRANSMEM 310..330
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 331..347
FT /note="Lumenal"
FT /evidence="ECO:0000305|PubMed:23091477"
FT TRANSMEM 348..368
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 369..379
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305|PubMed:23091477"
FT TRANSMEM 380..400
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 401..413
FT /note="Lumenal"
FT /evidence="ECO:0000305|PubMed:23091477"
FT TRANSMEM 414..434
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 435..446
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305|PubMed:23091477"
FT TRANSMEM 447..467
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 468..472
FT /note="Lumenal"
FT /evidence="ECO:0000305|PubMed:23091477"
SQ SEQUENCE 472 AA; 51877 MW; 9CE17F1E23844A45 CRC64;
MSLTQSAKLH IIHQSIGKIT TRLYDTSPFH GDSHCKQRRV KDNCIQFNMN ISNVLKCYSE
IESFSINQRI RRSISLFLSI LRSFYSMRMR LLDLFITSSI PVAKILLITG IGFYLALDQV
NILNHDARKQ LNNIVFYVFS PSLVASSLSE TITYESMVKM WFMPLNVLLT FIIGSFLGWI
VIKITKPPSH LRGIIVGCCA AGNLGNMPLI IIPAICNEKG SPFGDPESCE KFGLGYIALS
MAIGAIYIWT YVYNLMRMLA NPAGETAINS TSSTMPLISP KVEVAEQVGT WGKVKQRVCS
VAEKINLRTI FAPSTIAALI ALAVGLNPLL RKLLVGNTAP LRVIEDSVSL LGDGAIPVLT
LIVGGNLLNG LRGSGINKSV IMGVVVVRYL LLPILGVFIV RGAHYLGLVT SEPLYQFVLL
LQYVVPPAMN LGTITQLFGS GESECSVILF WSYALASVSL TVWPTFFMWL VA
