PILS4_ARATH
ID PILS4_ARATH Reviewed; 415 AA.
AC Q9C9K4;
DT 08-JUN-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=Protein PIN-LIKES 4 {ECO:0000303|PubMed:22504182};
DE AltName: Full=Auxin efflux carrier-like protein 4 {ECO:0000303|PubMed:22504182};
GN Name=PILS4 {ECO:0000303|PubMed:22504182};
GN OrderedLocusNames=At1g76530 {ECO:0000312|Araport:AT1G76530};
GN ORFNames=F14G6.13 {ECO:0000312|EMBL:AAG51958.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION BY AUXIN, GENE FAMILY,
RP AND NOMENCLATURE.
RX PubMed=22504182; DOI=10.1038/nature11001;
RA Barbez E., Kubes M., Rolcik J., Beziat C., Pencik A., Wang B.,
RA Rosquete M.R., Zhu J., Dobrev P.I., Lee Y., Zazimalova E., Petrasek J.,
RA Geisler M., Friml J., Kleine-Vehn J.;
RT "A novel putative auxin carrier family regulates intracellular auxin
RT homeostasis in plants.";
RL Nature 485:119-122(2012).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=23091477; DOI=10.3389/fpls.2012.00227;
RA Feraru E., Vosolsobe S., Feraru M.I., Petrasek J., Kleine-Vehn J.;
RT "Evolution and structural diversification of PILS putative auxin carriers
RT in plants.";
RL Front. Plant Sci. 3:227-227(2012).
CC -!- FUNCTION: Involved in cellular auxin homeostasis by regulating auxin
CC metabolism. Regulates intracellular auxin accumulation at the
CC endoplasmic reticulum and thus auxin availability for nuclear auxin
CC signaling. {ECO:0000305|PubMed:22504182}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000305|PubMed:22504182}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Expressed in seedlings, rosette and cauline leaves,
CC stems, flowers and siliques. {ECO:0000269|PubMed:22504182}.
CC -!- INDUCTION: Up-regulated by auxin application.
CC {ECO:0000269|PubMed:22504182}.
CC -!- SIMILARITY: Belongs to the auxin efflux carrier (TC 2.A.69.2) family.
CC {ECO:0000305}.
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DR EMBL; AC015450; AAG51958.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE35856.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM58623.1; -; Genomic_DNA.
DR PIR; C96793; C96793.
DR RefSeq; NP_001319388.1; NM_001334730.1.
DR RefSeq; NP_001321045.1; NM_001334731.1.
DR AlphaFoldDB; Q9C9K4; -.
DR STRING; 3702.AT1G76530.1; -.
DR PaxDb; Q9C9K4; -.
DR PRIDE; Q9C9K4; -.
DR EnsemblPlants; AT1G76530.1; AT1G76530.1; AT1G76530.
DR EnsemblPlants; AT1G76530.2; AT1G76530.2; AT1G76530.
DR GeneID; 843986; -.
DR Gramene; AT1G76530.1; AT1G76530.1; AT1G76530.
DR Gramene; AT1G76530.2; AT1G76530.2; AT1G76530.
DR KEGG; ath:AT1G76530; -.
DR Araport; AT1G76530; -.
DR TAIR; locus:2011746; AT1G76530.
DR eggNOG; KOG2722; Eukaryota.
DR HOGENOM; CLU_044945_0_0_1; -.
DR InParanoid; Q9C9K4; -.
DR OMA; IIIIPAM; -.
DR OrthoDB; 1055578at2759; -.
DR PhylomeDB; Q9C9K4; -.
DR PRO; PR:Q9C9K4; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9C9K4; baseline and differential.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0009734; P:auxin-activated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0080162; P:endoplasmic reticulum to cytosol auxin transport; IEA:InterPro.
DR GO; GO:0009733; P:response to auxin; IEP:UniProtKB.
DR InterPro; IPR004776; Mem_trans.
DR InterPro; IPR045033; PILS1/3/4/5/7.
DR PANTHER; PTHR31651; PTHR31651; 1.
DR Pfam; PF03547; Mem_trans; 1.
PE 2: Evidence at transcript level;
KW Auxin signaling pathway; Endoplasmic reticulum; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..415
FT /note="Protein PIN-LIKES 4"
FT /id="PRO_0000436499"
FT TOPO_DOM 1..13
FT /note="Lumenal"
FT /evidence="ECO:0000305|PubMed:23091477"
FT TRANSMEM 14..34
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 35..44
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305|PubMed:23091477"
FT TRANSMEM 45..61
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 62..75
FT /note="Lumenal"
FT /evidence="ECO:0000305|PubMed:23091477"
FT TRANSMEM 76..96
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 97..106
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305|PubMed:23091477"
FT TRANSMEM 107..127
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 128..143
FT /note="Lumenal"
FT /evidence="ECO:0000305|PubMed:23091477"
FT TRANSMEM 144..161
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 162..244
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305|PubMed:23091477"
FT TRANSMEM 245..265
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 266..285
FT /note="Lumenal"
FT /evidence="ECO:0000305|PubMed:23091477"
FT TRANSMEM 286..306
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 307..322
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305|PubMed:23091477"
FT TRANSMEM 323..343
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 344..355
FT /note="Lumenal"
FT /evidence="ECO:0000305|PubMed:23091477"
FT TRANSMEM 356..376
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 377..389
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305|PubMed:23091477"
FT TRANSMEM 390..410
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 411..415
FT /note="Lumenal"
FT /evidence="ECO:0000305|PubMed:23091477"
SQ SEQUENCE 415 AA; 45586 MW; 0AFE8D469F145BA8 CRC64;
MKLLELFIAS SKPVVETLLI TSVGFYLALD TVNLLGHDAR KHLNNIVFYV FSPSLIGSRL
ADSVTYESLV KMWFMPVNVL LTFMIGSLLG WIVIVITKPP SQLRGLIISC CASGNLGTMP
LIIIPAICKE KGGPFGDSES CEKYGMGYVT LSMTAFFISV YKHDTNWYVS GGNGLLMDLY
INLMRVLSNS PVETHTHSIE SNYDDSCKVQ LISSKEEEKE EDNHQVGRWE EVKQRVVSLS
KKVNLGSIFA PATIAAIIAL VIGLITPLRN LIIGTVAPFR VIQDSLTLLG DGAIPAMTLI
LGGNLLKGMR RSEVRSSEMK NSCIIGVLVA RYILLPVSGV LLVRGAYKLD LVTSEPLYQF
VLLLQYAVPP AMNLGTKTQL FGAGESECSV IMLWTYSLAA VSLTVWPTFF MWLVT
