PILS7_ARATH
ID PILS7_ARATH Reviewed; 395 AA.
AC Q9FKY4;
DT 08-JUN-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 106.
DE RecName: Full=Protein PIN-LIKES 7 {ECO:0000303|PubMed:22504182};
DE AltName: Full=Auxin efflux carrier-like protein 7 {ECO:0000303|PubMed:22504182};
GN Name=PILS7 {ECO:0000303|PubMed:22504182};
GN OrderedLocusNames=At5g65980 {ECO:0000312|Araport:AT5G65980};
GN ORFNames=K2A18.4 {ECO:0000312|EMBL:BAB10403.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9679202; DOI=10.1093/dnares/5.2.131;
RA Kaneko T., Kotani H., Nakamura Y., Sato S., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. V. Sequence
RT features of the regions of 1,381,565 bp covered by twenty one physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:131-145(1998).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP FUNCTION, TISSUE SPECIFICITY, INDUCTION BY AUXIN, GENE FAMILY,
RP NOMENCLATURE, AND SUBCELLULAR LOCATION.
RX PubMed=22504182; DOI=10.1038/nature11001;
RA Barbez E., Kubes M., Rolcik J., Beziat C., Pencik A., Wang B.,
RA Rosquete M.R., Zhu J., Dobrev P.I., Lee Y., Zazimalova E., Petrasek J.,
RA Geisler M., Friml J., Kleine-Vehn J.;
RT "A novel putative auxin carrier family regulates intracellular auxin
RT homeostasis in plants.";
RL Nature 485:119-122(2012).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=23091477; DOI=10.3389/fpls.2012.00227;
RA Feraru E., Vosolsobe S., Feraru M.I., Petrasek J., Kleine-Vehn J.;
RT "Evolution and structural diversification of PILS putative auxin carriers
RT in plants.";
RL Front. Plant Sci. 3:227-227(2012).
CC -!- FUNCTION: Involved in cellular auxin homeostasis by regulating auxin
CC metabolism. Regulates intracellular auxin accumulation at the
CC endoplasmic reticulum and thus auxin availability for nuclear auxin
CC signaling. {ECO:0000269|PubMed:22504182}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000269|PubMed:22504182}; Multi-pass membrane protein
CC {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in seedlings, rosette and cauline leaves,
CC stems and flowers. {ECO:0000269|PubMed:22504182}.
CC -!- INDUCTION: Up-regulated by auxin application.
CC {ECO:0000269|PubMed:22504182}.
CC -!- SIMILARITY: Belongs to the auxin efflux carrier (TC 2.A.69.2) family.
CC {ECO:0000305}.
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DR EMBL; AB011474; BAB10403.1; -; Genomic_DNA.
DR EMBL; CP002688; AED98134.1; -; Genomic_DNA.
DR RefSeq; NP_201399.1; NM_125995.2.
DR AlphaFoldDB; Q9FKY4; -.
DR STRING; 3702.AT5G65980.1; -.
DR PaxDb; Q9FKY4; -.
DR PRIDE; Q9FKY4; -.
DR ProteomicsDB; 234909; -.
DR EnsemblPlants; AT5G65980.1; AT5G65980.1; AT5G65980.
DR GeneID; 836728; -.
DR Gramene; AT5G65980.1; AT5G65980.1; AT5G65980.
DR KEGG; ath:AT5G65980; -.
DR Araport; AT5G65980; -.
DR TAIR; locus:2156847; AT5G65980.
DR eggNOG; KOG2722; Eukaryota.
DR HOGENOM; CLU_044945_0_0_1; -.
DR InParanoid; Q9FKY4; -.
DR OMA; WVIIRAS; -.
DR OrthoDB; 1055578at2759; -.
DR PhylomeDB; Q9FKY4; -.
DR PRO; PR:Q9FKY4; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FKY4; baseline and differential.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IDA:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0009734; P:auxin-activated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0080162; P:endoplasmic reticulum to cytosol auxin transport; IEA:InterPro.
DR GO; GO:0009733; P:response to auxin; IEP:UniProtKB.
DR InterPro; IPR004776; Mem_trans.
DR InterPro; IPR045033; PILS1/3/4/5/7.
DR PANTHER; PTHR31651; PTHR31651; 1.
DR Pfam; PF03547; Mem_trans; 1.
PE 2: Evidence at transcript level;
KW Auxin signaling pathway; Endoplasmic reticulum; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..395
FT /note="Protein PIN-LIKES 7"
FT /id="PRO_0000436502"
FT TOPO_DOM 1..8
FT /note="Lumenal"
FT /evidence="ECO:0000305|PubMed:23091477"
FT TRANSMEM 9..29
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 30..45
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305|PubMed:23091477"
FT TRANSMEM 46..66
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 67..73
FT /note="Lumenal"
FT /evidence="ECO:0000305|PubMed:23091477"
FT TRANSMEM 74..94
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 95..106
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305|PubMed:23091477"
FT TRANSMEM 107..127
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 128..142
FT /note="Lumenal"
FT /evidence="ECO:0000305|PubMed:23091477"
FT TRANSMEM 143..163
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 164..232
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305|PubMed:23091477"
FT TRANSMEM 233..253
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 254..272
FT /note="Lumenal"
FT /evidence="ECO:0000305|PubMed:23091477"
FT TRANSMEM 273..293
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 294..302
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305|PubMed:23091477"
FT TRANSMEM 303..323
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 324..340
FT /note="Lumenal"
FT /evidence="ECO:0000305|PubMed:23091477"
FT TRANSMEM 341..361
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 362..369
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305|PubMed:23091477"
FT TRANSMEM 370..390
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 391..395
FT /note="Lumenal"
FT /evidence="ECO:0000305|PubMed:23091477"
SQ SEQUENCE 395 AA; 43074 MW; 9EB4A8E331255DD2 CRC64;
MGFLELLEVA SMPIVQVLLI SVLGAFLATD YCSLLSADTR RSVNKLVFVV FTPCIMFANL
AETVTLQDII SWWFMPINVG ITFLVGGILG WLVVKLLNPK PQLHGLIIAT CASGNMGNLM
LILVPAICDE EGSPFGNRSV CRSIGLSYAS FSMALGGFYI WTYSYQLVRS SATQFRALEA
AGLVKSPNKD IDSDPHALLL KPHQNQDLEI QGKQKVSTRT YIKDLLHQIL EELFAPPTIG
AILGFVFGAT NWLRNLIIGE NAPLRVIQDS VKLLGEGTIP CITLILGGNL IQGLRSSAVK
KSVIVGVIIV RYILLPVVGV GVVQLAGNLG YLPPDPLFRY VLMLQFALPP AMNISTMAQL
FDVAQDECSV IFLWTYLVAS LALTVWSTIF LSILS
