PILY1_PSEAB
ID PILY1_PSEAB Reviewed; 1158 AA.
AC Q02GC2; Q7WZN9; S0J269;
DT 04-FEB-2015, integrated into UniProtKB/Swiss-Prot.
DT 14-NOV-2006, sequence version 1.
DT 03-AUG-2022, entry version 65.
DE RecName: Full=Type IV pilus biogenesis factor PilY1 {ECO:0000250|UniProtKB:S0HPF7};
DE AltName: Full=Pilus-associated adhesin PilY1 {ECO:0000250|UniProtKB:Q9HVM8};
DE Flags: Precursor;
GN Name=pilY1 {ECO:0000312|EMBL:ABJ13934.1};
GN OrderedLocusNames=PA14_60310 {ECO:0000312|EMBL:ABJ13934.1};
OS Pseudomonas aeruginosa (strain UCBPP-PA14).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208963 {ECO:0000312|EMBL:ABJ13934.1};
RN [1] {ECO:0000312|EMBL:AAP81276.1}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=UCBPP-PA14 {ECO:0000312|EMBL:AAP81276.1};
RX PubMed=14983043; DOI=10.1073/pnas.0304622101;
RA He J., Baldini R.L., Deziel E., Saucier M., Zhang Q., Liberati N.T.,
RA Lee D., Urbach J., Goodman H.M., Rahme L.G.;
RT "The broad host range pathogen Pseudomonas aeruginosa strain PA14 carries
RT two pathogenicity islands harboring plant and animal virulence genes.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:2530-2535(2004).
RN [2] {ECO:0000312|EMBL:ABJ13934.1, ECO:0000312|Proteomes:UP000000653}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=UCBPP-PA14 {ECO:0000312|EMBL:ABJ13934.1,
RC ECO:0000312|Proteomes:UP000000653};
RX PubMed=17038190; DOI=10.1186/gb-2006-7-10-r90;
RA Lee D.G., Urbach J.M., Wu G., Liberati N.T., Feinbaum R.L., Miyata S.,
RA Diggins L.T., He J., Saucier M., Deziel E., Friedman L., Li L., Grills G.,
RA Montgomery K., Kucherlapati R., Rahme L.G., Ausubel F.M.;
RT "Genomic analysis reveals that Pseudomonas aeruginosa virulence is
RT combinatorial.";
RL Genome Biol. 7:R90.1-R90.14(2006).
RN [3] {ECO:0000312|EMBL:EOT21828.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=UCBPP-PA14 {ECO:0000312|EMBL:EOT21828.1};
RG The Broad Institute Genomics Platform;
RG The Broad Institute Genome Sequencing Center for Infectious Disease;
RA Hung D., Lory S., Poulsen B., Penaranda C., Ausubel F., Walker B.,
RA Young S., Zeng Q., Gargeya S., Fitzgerald M., Haas B., Abouelleil A.,
RA Allen A.W., Alvarado L., Arachchi H.M., Berlin A.M., Chapman S.B.,
RA Gainer-Dewar J., Goldberg J., Griggs A., Gujja S., Hansen M., Howarth C.,
RA Imamovic A., Ireland A., Larimer J., McCowan C., Murphy C., Pearson M.,
RA Poon T.W., Priest M., Roberts A., Saif S., Shea T., Sisk P., Sykes S.,
RA Wortman J., Nusbaum C., Birren B.;
RT "The genome sequence of Pseudomonas aeruginosa PA14.";
RL Submitted (MAY-2013) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP DISRUPTION PHENOTYPE.
RC STRAIN=UCBPP-PA14 {ECO:0000303|PubMed:19054330};
RX PubMed=19054330; DOI=10.1111/j.1365-2958.2008.06559.x;
RA Bohn Y.S., Brandes G., Rakhimova E., Horatzek S., Salunkhe P., Munder A.,
RA van Barneveld A., Jordan D., Bredenbruch F., Haeussler S., Riedel K.,
RA Eberl L., Jensen P., Bjarnsholt T., Moser C., Hoiby N., Tuemmler B.,
RA Wiehlmann L.;
RT "Multiple roles of Pseudomonas aeruginosa TBCF10839 PilY1 in motility,
RT transport and infection.";
RL Mol. Microbiol. 71:730-747(2009).
RN [5]
RP SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, MISCELLANEOUS, AND MUTAGENESIS
RP OF THR-270 AND 310-ASP-GLY-311.
RC STRAIN=UCBPP-PA14 {ECO:0000303|PubMed:20233936};
RX PubMed=20233936; DOI=10.1128/jb.01642-09;
RA Kuchma S.L., Ballok A.E., Merritt J.H., Hammond J.H., Lu W.,
RA Rabinowitz J.D., O'Toole G.A.;
RT "Cyclic-di-GMP-mediated repression of swarming motility by Pseudomonas
RT aeruginosa: the pilY1 gene and its impact on surface-associated
RT behaviors.";
RL J. Bacteriol. 192:2950-2964(2010).
RN [6]
RP INDUCTION, DISRUPTION PHENOTYPE, AND MISCELLANEOUS.
RC STRAIN=UCBPP-PA14 {ECO:0000303|PubMed:22865844};
RX PubMed=22865844; DOI=10.1128/jb.00899-12;
RA Kuchma S.L., Griffin E.F., O'Toole G.A.;
RT "Minor pilins of the type IV pilus system participate in the negative
RT regulation of swarming motility.";
RL J. Bacteriol. 194:5388-5403(2012).
RN [7]
RP FUNCTION, INDUCTION BY SURFACE ATTACHMENT, DISRUPTION PHENOTYPE,
RP PHARMACEUTICAL, AND VWFA HOMOLOGY REGION.
RC STRAIN=UCBPP-PA14 {ECO:0000303|PubMed:25385640};
RX PubMed=25385640; DOI=10.1073/pnas.1415712111;
RA Siryaporn A., Kuchma S.L., O'Toole G.A., Gitai Z.;
RT "Surface attachment induces Pseudomonas aeruginosa virulence.";
RL Proc. Natl. Acad. Sci. U.S.A. 111:16860-16865(2014).
CC -!- FUNCTION: Involved in pilus assembly, twitching motility and adhesion
CC to host cells. Primes type IV pili (T4P) assembly and is required for
CC inclusion of minor pilins PilV, PilW and PilX to the surface pili.
CC Stabilizes assembled pilus fibers likely by antagonizing retraction
CC mediated by PilT. Calcium-binding and calcium release by PilY1 seem to
CC be essential for twitching motility and for regulation of pilus
CC retraction dynamics of PilT (By similarity). Regulates surface-
CC activated virulence possibly by acting as a surface-attachment
CC mechanosensor (PubMed:25385640). {ECO:0000250|UniProtKB:Q9HVM8,
CC ECO:0000250|UniProtKB:S0HPF7, ECO:0000269|PubMed:25385640}.
CC -!- SUBCELLULAR LOCATION: Fimbrium {ECO:0000250|UniProtKB:S0HPF7}. Membrane
CC {ECO:0000269|PubMed:20233936}. Cytoplasm, cytosol
CC {ECO:0000269|PubMed:20233936}. Note=Colocalizes with the T4P fraction
CC when surface pili are present. Sheared surface fraction when PilV, PilW
CC and PilX are also present. {ECO:0000250|UniProtKB:Q9HVM8,
CC ECO:0000250|UniProtKB:S0HPF7}.
CC -!- INDUCTION: By the swarm surface growth conditions relative to liquid
CC growth conditions (PubMed:22865844). By surface attachment
CC (PubMed:25385640). {ECO:0000269|PubMed:22865844,
CC ECO:0000269|PubMed:25385640}.
CC -!- DOMAIN: Contains a region (residues 173-365) weakly homologous to
CC eukaryotic mechanically sensitive Von Willebrand Factor a (VWFa).
CC {ECO:0000305|PubMed:25385640}.
CC -!- DISRUPTION PHENOTYPE: Loss of twitching motility, predesposes to
CC autolysis and impairs the secretion of quinolones and pyocyanine, but
CC on the other hand promotes growth in stationary phase and bacterial
CC survival in murine airway infection models (PubMed:19054330). Has
CC reduced congo red (CR) binding which is a measure of Pel
CC exopolysaccharide (EPS) production, and shows both increased swarming
CC motility and an altered swarming pattern compared to wild-type.
CC Suppresses cyclic-di-GMP (c-di-GMP) degrading phosphodiesterase bifA
CC deletion mutant's swarming defects and eliminates its hyperbiofilm
CC formation although the global pools of c-di-GMP remain unchanged
CC (PubMed:20233936). PilY1 single mutant does not exhibit any changes in
CC rhamnolipid production or in levels of the flagellar subunit FliC
CC (PubMed:22865844). Defective for surface-activated virulence toward
CC amoebae (PubMed:25385640). {ECO:0000269|PubMed:19054330,
CC ECO:0000269|PubMed:20233936, ECO:0000269|PubMed:22865844,
CC ECO:0000269|PubMed:25385640}.
CC -!- PHARMACEUTICAL: Targeting PilY1-mediated mechanosensation as a global
CC virulence regulator could be an attractive therapeutic strategy in the
CC development of treatments for infections caused by P.aeruginosa.
CC {ECO:0000305|PubMed:25385640}.
CC -!- MISCELLANEOUS: Overexpression of PilY1 represses swarming motility in a
CC pilus-independent manner and the repression specifically requires the
CC diguanylate cyclase (DGC) SadC but not other DGCs (PubMed:20233936).
CC Increased c-di-GMP levels upon PilY1 overexpression in surface-grown
CC cells, but only in the presence of SadC (PubMed:22865844).
CC {ECO:0000269|PubMed:20233936, ECO:0000269|PubMed:22865844}.
CC -!- SIMILARITY: Belongs to the PilY1 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAP81276.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AY273871; AAP81276.1; ALT_INIT; Genomic_DNA.
DR EMBL; CP000438; ABJ13934.1; -; Genomic_DNA.
DR EMBL; ASWV01000003; EOT21828.1; -; Genomic_DNA.
DR RefSeq; WP_003141555.1; NZ_CP034244.1.
DR AlphaFoldDB; Q02GC2; -.
DR SMR; Q02GC2; -.
DR PRIDE; Q02GC2; -.
DR EnsemblBacteria; ABJ13934; ABJ13934; PA14_60310.
DR KEGG; pau:PA14_60310; -.
DR PATRIC; fig|652611.13.peg.149; -.
DR HOGENOM; CLU_001890_1_1_6; -.
DR OMA; LWHAAVN; -.
DR BioCyc; PAER208963:G1G74-5098-MON; -.
DR Proteomes; UP000000653; Chromosome.
DR GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0009289; C:pilus; IEA:UniProtKB-SubCell.
DR GO; GO:0005509; F:calcium ion binding; ISS:UniProtKB.
DR Gene3D; 3.40.50.410; -; 1.
DR InterPro; IPR008707; PilC_beta_prop_dom.
DR InterPro; IPR011047; Quinoprotein_ADH-like_supfam.
DR InterPro; IPR036465; vWFA_dom_sf.
DR Pfam; PF05567; Neisseria_PilC; 1.
DR SUPFAM; SSF50998; SSF50998; 1.
PE 1: Evidence at protein level;
KW Calcium; Cytoplasm; Fimbrium; Fimbrium biogenesis; Membrane; Metal-binding;
KW Pharmaceutical; Signal.
FT SIGNAL 1..29
FT /evidence="ECO:0000255"
FT CHAIN 30..1158
FT /note="Type IV pilus biogenesis factor PilY1"
FT /evidence="ECO:0000255"
FT /id="PRO_0000431915"
FT BINDING 841
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000250|UniProtKB:S0HPF7"
FT BINDING 843
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000250|UniProtKB:S0HPF7"
FT BINDING 845
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000250|UniProtKB:S0HPF7"
FT BINDING 847
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000250|UniProtKB:S0HPF7"
FT BINDING 849
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000250|UniProtKB:S0HPF7"
FT MUTAGEN 270
FT /note="T->A: Inhibits swarming as wild-type."
FT /evidence="ECO:0000269|PubMed:20233936"
FT MUTAGEN 310..311
FT /note="DG->AA: Only partial swarming inhibition compared to
FT the complete inhibition of the wild-type and
FT mislocalization."
FT /evidence="ECO:0000269|PubMed:20233936"
SQ SEQUENCE 1158 AA; 126693 MW; 856D13A0DB5BF252 CRC64;
MIHQITRAGK SLLAAGCTLS ILFASDSYAA TALNVSQQPL FLTQGVAPNL LFTLDDSGSM
AWAYVPDGIS GNSGRAGRSS DYNALYYNPD YAYQVPKKLT LSGDQIIVSD YPVPRFTAAW
QDGYAQGSTT NLSNNYRPQW GTGWLGCIDS SCNTGRAYYY TYKVSASCPA QPVSSSNSCY
TYNALPTSQE SNFAIWYSYY RNRILATKTA ANLAFYSLPE NVRLTWGALN TCSIGANSRS
CQNNALLQFN KQHKINFFNW LANSPASGGT PLHAALDRAG RFLQTNGTAY TTEDGKTYSC
RASYHIMMTD GIWNGRNVTP GNLDNQNQTF PDSTLYRPQP PYADSNASSL ADLAFKYWTT
DLRPSIDNDL KPFMAYKSGD DSKDYWDPRN NPATWQHMVN FTVGLGLSYS LTLNSAPTWT
GSTFGNYEEL MAGSKAWPSV DNDAAPGNVY DLWHAAINSR GDFFSAESPD SLVQAFNKIL
TRISERNTSS SKPAMTSALQ DDGTGDKLIR YSYQSSFASD KNWAGDLIRY KVESTSTGST
KTQEWSAGAL LDNRAPATRN IYIASNSGTN RLKPFTWSNI EGSQLATWLN RNPDKDNQAD
TKGAQRVDFI RGQQNMDGFR QRQAVLGDIV HSSPAVVGPA QYLTYLANPI EPSGDYGTFK
TEADQRSPRV YVGSNDGMLH GFNIKTGVEE FAFIPTAVFE KLNKLTGISY QGGAHQYFVD
ATPVVSDAFF DGAWHTVLIG TLGAGGRGLF ALDVTKPDDV KLLWEYDSST DSDLGYTFSK
PTVARLHSGQ WAVVTGNGYG SDNDKAALLL IDLKKGTLIK KLEVQSERGI ANGLSTPRLA
DNNSDGIADY AYAGDLQGNI WRFDLIGNTR NDDPDTNTSI NPFKPGDVDP SAFRVSFSGA
PLFRARADNN TRQPITAPPT LVRHPSRKGY IVIVGTGKYF EDDDAQADTS RAMTLYGIWD
RQTKGESANS TPTIDRNALT AQTMTTEANS TFGSVNRNIR LISQNPVKWY KDGATGTANS
DVASYGWRLN LEVNSSKKGE MMIEDMFAAG QVLLLQTLTP NDDPCDSGST SWTYGLNPYT
GGRTSFTVFD LKRAGIVDSG SDYNGSVVSA FQQDGLGGLA ITQNEQRQSE ACTGDECIIF
NPSDKSNGRQ TWRVVEEK
