PILY1_PSEAE
ID PILY1_PSEAE Reviewed; 1161 AA.
AC Q9HVM8; Q51536;
DT 04-FEB-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 101.
DE RecName: Full=Type IV pilus biogenesis factor PilY1 {ECO:0000250|UniProtKB:S0HPF7};
DE AltName: Full=Pilus-associated adhesin PilY1 {ECO:0000303|PubMed:25389296};
DE Flags: Precursor;
GN Name=pilY1 {ECO:0000312|EMBL:AAA93502.1, ECO:0000312|EMBL:AAG07942.1};
GN OrderedLocusNames=PA4554 {ECO:0000312|EMBL:AAG07942.1};
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964 {ECO:0000312|EMBL:AAG07942.1};
RN [1] {ECO:0000312|PIR:S72645}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1 {ECO:0000303|PubMed:8899718};
RX PubMed=8899718; DOI=10.1111/j.1365-2958.1996.tb02665.x;
RA Alm R.A., Hallinan J.P., Watson A.A., Mattick J.S.;
RT "Fimbrial biogenesis genes of Pseudomonas aeruginosa: pilW and pilX
RT increase the similarity of type 4 fimbriae to the GSP protein-secretion
RT systems and pilY1 encodes a gonococcal PilC homologue.";
RL Mol. Microbiol. 22:161-173(1996).
RN [2] {ECO:0000312|EMBL:AAG07942.1, ECO:0000312|Proteomes:UP000002438}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1 {ECO:0000312|Proteomes:UP000002438};
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
RN [3]
RP FUNCTION, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=25389296; DOI=10.1074/jbc.m114.616904;
RA Nguyen Y., Sugiman-Marangos S., Harvey H., Bell S.D., Charlton C.L.,
RA Junop M.S., Burrows L.L.;
RT "Pseudomonas aeruginosa minor pilins prime type IVa pilus assembly and
RT promote surface display of the PilY1 adhesin.";
RL J. Biol. Chem. 290:601-611(2015).
CC -!- FUNCTION: Involved in pilus assembly, twitching motility and adhesion
CC to host cells. Primes type IV pili (T4P) assembly and is required for
CC inclusion of minor pilins PilV, PilW and PilX to the surface pili
CC (PubMed:25389296). Stabilizes assembled pilus fibers likely by
CC antagonizing retraction mediated by PilT. Calcium-binding and calcium
CC release by PilY1 seem to be essential for twitching motility and for
CC regulation of pilus retraction dynamics of PilT (By similarity).
CC {ECO:0000250|UniProtKB:S0HPF7, ECO:0000269|PubMed:25389296}.
CC -!- SUBUNIT: Interacts (via C-terminus) with host integrins alpha-V/beta-3
CC (ITGAV/ITGB3) and alpha-V/beta-5 (ITGAV/ITGB5).
CC {ECO:0000250|UniProtKB:S0HPF7}.
CC -!- SUBCELLULAR LOCATION: Fimbrium {ECO:0000250|UniProtKB:S0HPF7}. Membrane
CC {ECO:0000250|UniProtKB:S0HPF7}. Cytoplasm, cytosol
CC {ECO:0000250|UniProtKB:Q02GC2}. Note=Colocalizes with the T4P fraction
CC when surface pili are present (By similarity). Sheared surface fraction
CC when PilV, PilW and PilX are also present (PubMed:25389296).
CC {ECO:0000250|UniProtKB:S0HPF7, ECO:0000269|PubMed:25389296}.
CC -!- DISRUPTION PHENOTYPE: Pilus assembly severely impaired. Retraction-
CC deficient. Prevents incorporation of PilE into pili.
CC {ECO:0000269|PubMed:25389296}.
CC -!- MISCELLANEOUS: Residues 598-606 comprise a calcium-binding site which
CC together with the other one (residues 849-857) seems important for
CC interaction with integrins of the host. {ECO:0000250|UniProtKB:S0HPF7}.
CC -!- SIMILARITY: Belongs to the PilY1 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA93502.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC Sequence=AAA93502.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; L76605; AAA93502.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AE004091; AAG07942.1; -; Genomic_DNA.
DR PIR; D83076; D83076.
DR PIR; S72645; S72645.
DR RefSeq; NP_253244.1; NC_002516.2.
DR RefSeq; WP_003115287.1; NZ_QZGE01000004.1.
DR AlphaFoldDB; Q9HVM8; -.
DR SMR; Q9HVM8; -.
DR STRING; 287.DR97_1858; -.
DR TCDB; 9.A.21.1.2; the comc dna uptake competence (comc) family.
DR PaxDb; Q9HVM8; -.
DR PRIDE; Q9HVM8; -.
DR EnsemblBacteria; AAG07942; AAG07942; PA4554.
DR GeneID; 877859; -.
DR KEGG; pae:PA4554; -.
DR PATRIC; fig|208964.12.peg.4766; -.
DR PseudoCAP; PA4554; -.
DR HOGENOM; CLU_001890_1_1_6; -.
DR InParanoid; Q9HVM8; -.
DR OMA; LWHAAVN; -.
DR PhylomeDB; Q9HVM8; -.
DR BioCyc; PAER208964:G1FZ6-4647-MON; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0009289; C:pilus; IEA:UniProtKB-SubCell.
DR GO; GO:0005509; F:calcium ion binding; ISS:UniProtKB.
DR GO; GO:0043107; P:type IV pilus-dependent motility; IMP:PseudoCAP.
DR InterPro; IPR008707; PilC_beta_prop_dom.
DR InterPro; IPR011047; Quinoprotein_ADH-like_supfam.
DR Pfam; PF05567; Neisseria_PilC; 1.
DR SUPFAM; SSF50998; SSF50998; 1.
PE 3: Inferred from homology;
KW Calcium; Cytoplasm; Fimbrium; Fimbrium biogenesis; Membrane; Metal-binding;
KW Reference proteome; Signal.
FT SIGNAL 1..30
FT /evidence="ECO:0000255"
FT CHAIN 31..1161
FT /note="Type IV pilus biogenesis factor PilY1"
FT /evidence="ECO:0000255"
FT /id="PRO_0000431916"
FT REGION 617..619
FT /note="Integrin-binding motif RGD"
FT /evidence="ECO:0000250|UniProtKB:S0HPF7"
FT REGION 1136..1161
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1136..1150
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 598
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:S0HPF7"
FT BINDING 600
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:S0HPF7"
FT BINDING 602
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:S0HPF7"
FT BINDING 606
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:S0HPF7"
FT BINDING 849
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000250|UniProtKB:S0HPF7"
FT BINDING 851
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000250|UniProtKB:S0HPF7"
FT BINDING 853
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000250|UniProtKB:S0HPF7"
FT BINDING 855
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000250|UniProtKB:S0HPF7"
FT BINDING 857
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000250|UniProtKB:S0HPF7"
FT CONFLICT 128
FT /note="S -> R (in Ref. 1; AAA93502)"
FT /evidence="ECO:0000305"
FT CONFLICT 169
FT /note="C -> W (in Ref. 1; AAA93502)"
FT /evidence="ECO:0000305"
FT CONFLICT 203
FT /note="A -> R (in Ref. 1; AAA93502)"
FT /evidence="ECO:0000305"
FT CONFLICT 213..214
FT /note="YS -> FR (in Ref. 1; AAA93502)"
FT /evidence="ECO:0000305"
FT CONFLICT 239
FT /note="S -> G (in Ref. 1; AAA93502)"
FT /evidence="ECO:0000305"
FT CONFLICT 255
FT /note="H -> Q (in Ref. 1; AAA93502)"
FT /evidence="ECO:0000305"
FT CONFLICT 273
FT /note="P -> H (in Ref. 1; AAA93502)"
FT /evidence="ECO:0000305"
FT CONFLICT 281
FT /note="A -> G (in Ref. 1; AAA93502)"
FT /evidence="ECO:0000305"
FT CONFLICT 388
FT /note="A -> D (in Ref. 1; AAA93502)"
FT /evidence="ECO:0000305"
FT CONFLICT 392
FT /note="A -> G (in Ref. 1; AAA93502)"
FT /evidence="ECO:0000305"
FT CONFLICT 403
FT /note="T -> I (in Ref. 1; AAA93502)"
FT /evidence="ECO:0000305"
FT CONFLICT 417
FT /note="T -> N (in Ref. 1; AAA93502)"
FT /evidence="ECO:0000305"
FT CONFLICT 549
FT /note="K -> N (in Ref. 1; AAA93502)"
FT /evidence="ECO:0000305"
FT CONFLICT 594
FT /note="Q -> K (in Ref. 1; AAA93502)"
FT /evidence="ECO:0000305"
FT CONFLICT 845
FT /note="P -> L (in Ref. 1; AAA93502)"
FT /evidence="ECO:0000305"
FT CONFLICT 892
FT /note="A -> T (in Ref. 1; AAA93502)"
FT /evidence="ECO:0000305"
FT CONFLICT 1006
FT /note="A -> G (in Ref. 1; AAA93502)"
FT /evidence="ECO:0000305"
FT CONFLICT 1083
FT /note="S -> R (in Ref. 1; AAA93502)"
FT /evidence="ECO:0000305"
FT CONFLICT 1090
FT /note="A -> G (in Ref. 1; AAA93502)"
FT /evidence="ECO:0000305"
FT CONFLICT 1102
FT /note="S -> R (in Ref. 1; AAA93502)"
FT /evidence="ECO:0000305"
FT CONFLICT 1106
FT /note="Q -> R (in Ref. 1; AAA93502)"
FT /evidence="ECO:0000305"
FT CONFLICT 1109..1110
FT /note="AV -> GN (in Ref. 1; AAA93502)"
FT /evidence="ECO:0000305"
FT CONFLICT 1129
FT /note="G -> A (in Ref. 1; AAA93502)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1161 AA; 126584 MW; 027E092E1084141F CRC64;
MKSVLHQIGK TSLAAALSGA VLLSAQTTHA AALSVSQQPL MLIQGVAPNM LVTLDDSGSM
AFAYAPDSIS GYGNYTFFAS NSFNPMYFDP NTQYKLPKKL TLVNGQVQIQ DYPAPNFSSA
WRNGFTRSGS INLSNSYKVT IEYGRGYDKE STIKADAAYY YDFTGSSSCN RTNQACYTRR
YVSTEQRQNF ANWYSFYRTR ALATQTAANL AFYSLPENAR VSWQLLNDSN CNQMGSGSSS
GNCFSNYLRD FTGQHRVNFF NWLEKLSVNG GTPLRQAMTR AGEFLKKTGV NGPYAYRPGT
QTAPEYSCRG SYHILMTDGL WNNDSANVGN ADSTARNLPD GKSYSSQTPY RDGTFDTLAD
QAFHYWATDA RPDIDDNIKP YIPYPDQANP SAEYWNPRND PATWQHMVTY TLGLGLTTSL
TSPRWEGSTF SGGYNDIVAG NLSWPRASNN DSNNVYDLWH AAVNSRGEFF SADSPDQLVA
AFQDILNRIS GKDLPASRPA ISSSLQEDDT GDKLTRFAYQ TSFASDKNWA GDLTRYSLTT
QDKATVQTKL WSAQSILDAM PNGGAGRKIM MAGSGTSGLK EFTWGSLSAD QQRQLNRDPD
RNDVADTKGQ DRVAFLRGDR RKENSDNFRT RNSILGDIIN SSPATVGKAQ YLTYLAQPIE
PSGNYSTFAE AQKTRAPRVY VGANDGMLHG FDTDGNETFA FIPSAVFEKL HKLTARGYQG
GAHQFYVDGS PVVADAFFGG AWHTVLIGSL RAGGKGLFAL DVTDPANIKL LWEIGVDQEP
DLGYSFPKPT VARLHNGKWA VVTGNGYSSL NDKAALLIID LETGAITRKL EVTGRTGVPN
GLSSPRLADN NSDGVADYAY AGDLQGNLWR FDLIAGKVNQ DDPFSRANDG PAVASSFRVS
FGGQPLYSAV DSAGAAQAIT AAPSLVRHPT RKGYIVIFGT GKYFENADAR ADTSRAQTLY
GIWDQQTKGE AAGSTPRLTR GNLQQQTLDL QADSTFASTA RTIRIASQNP VNWLNNDGST
KQSGWYLDFM VNGTLKGEML IEDMIAIGQV VLLQTITPND DPCADGASNW TYGLDPYTGG
RTSFTVFDLA RQGVVDSKSD YSYNKQNVAV SGTEQKGLGG LTLSTNEQGN PEVCSSGECL
TVNPGPNTRG RQNWRPIEGK N
