A62F_DROME
ID A62F_DROME Reviewed; 115 AA.
AC O46202;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1998, sequence version 1.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=Accessory gland protein Acp62F;
DE Flags: Precursor;
GN Name=Acp62F; ORFNames=CG1262;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE, FUNCTION, AND TISSUE SPECIFICITY.
RC STRAIN=Canton-S; TISSUE=Male accessory gland;
RX PubMed=9474779; DOI=10.1016/s0965-1748(97)00056-8;
RA Wolfner M.F., Harada H.A., Bertram M.J., Stelick T.J., Kraus K.W.,
RA Kalb J.M., Lung Y.O., Neubaum D.M., Park M., Tram U.K.;
RT "New genes for male accessory gland proteins in Drosophila melanogaster.";
RL Insect Biochem. Mol. Biol. 27:825-834(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=NFS 5.1, NFS 5.2, NFS 5.3, NFS 5.4, NFS 6.1, NFS 6.2, NFS 6.3,
RC NFS 6.4, NFS 7.8, SFS 1.1, SFS 1.2, SFS 1.3, SFS 1.4, SFS 2.2, SFS 2.3,
RC SFS 2.4, SFS 3.1, SFS 3.2, SFS 3.3, and SFS 3.4;
RX PubMed=14761057; DOI=10.1554/03-335;
RA Panhuis T.M., Swanson W.J., Nunney L.;
RT "Population genetics of accessory gland proteins and sexual behavior in
RT Drosophila melanogaster populations from Evolution Canyon.";
RL Evolution 57:2785-2791(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE OF 7-111.
RC STRAIN=Zim62H-12C, Zim62H-16C, Zim62H-28C, Zim62H-30C, Zim62H-34C,
RC Zim62I-10C, Zim62I-17C, Zim62I-18C, Zim62I-53C, and Zim62I-5C;
RX PubMed=11102381; DOI=10.1093/genetics/156.4.1879;
RA Begun D.J., Whitley P., Todd B.L., Waldrip-Dail H.M., Clark A.G.;
RT "Molecular population genetics of male accessory gland proteins in
RT Drosophila.";
RL Genetics 156:1879-1888(2000).
RN [6]
RP TISSUE SPECIFICITY.
RX PubMed=10612039; DOI=10.1016/s0965-1748(99)00078-8;
RA Lung O., Wolfner M.F.;
RT "Drosophila seminal fluid proteins enter the circulatory system of the
RT mated female fly by crossing the posterior vaginal wall.";
RL Insect Biochem. Mol. Biol. 29:1043-1052(1999).
CC -!- FUNCTION: Responsible for physiological and behavioral changes in mated
CC female flies. May contribute to the toxicity of seminal fluid and the
CC decreased life-span of mated females. May also affect neuromuscular
CC events after mating concerning sperm storage and egg release.
CC {ECO:0000269|PubMed:9474779}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Produced in the male genital tract (at protein
CC level) (PubMed:10612039). Present in seminal fluid (PubMed:9474779).
CC Transferred to the female genital tract within the first 3 minutes
CC after the start of mating (ASM) and crosses the posterior vaginal wall
CC into the female hemolymph 5 minutes ASM (at protein level)
CC (PubMed:10612039). {ECO:0000269|PubMed:10612039,
CC ECO:0000269|PubMed:9474779}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; U85763; AAB96387.1; -; mRNA.
DR EMBL; AY344325; AAR05044.1; -; Genomic_DNA.
DR EMBL; AY344326; AAR05045.1; -; Genomic_DNA.
DR EMBL; AY344327; AAR05046.1; -; Genomic_DNA.
DR EMBL; AY344328; AAR05047.1; -; Genomic_DNA.
DR EMBL; AY344329; AAR05048.1; -; Genomic_DNA.
DR EMBL; AY344330; AAR05049.1; -; Genomic_DNA.
DR EMBL; AY344331; AAR05050.1; -; Genomic_DNA.
DR EMBL; AY344332; AAR05051.1; -; Genomic_DNA.
DR EMBL; AY344333; AAR05052.1; -; Genomic_DNA.
DR EMBL; AY344334; AAR05053.1; -; Genomic_DNA.
DR EMBL; AY344335; AAR05054.1; -; Genomic_DNA.
DR EMBL; AY344336; AAR05055.1; -; Genomic_DNA.
DR EMBL; AY344337; AAR05056.1; -; Genomic_DNA.
DR EMBL; AY344338; AAR05057.1; -; Genomic_DNA.
DR EMBL; AY344339; AAR05058.1; -; Genomic_DNA.
DR EMBL; AY344340; AAR05059.1; -; Genomic_DNA.
DR EMBL; AY344341; AAR05060.1; -; Genomic_DNA.
DR EMBL; AY344342; AAR05061.1; -; Genomic_DNA.
DR EMBL; AY344343; AAR05062.1; -; Genomic_DNA.
DR EMBL; AY344344; AAR05063.1; -; Genomic_DNA.
DR EMBL; AE014296; AAF47683.1; -; Genomic_DNA.
DR EMBL; AY010608; AAG35367.1; -; Genomic_DNA.
DR EMBL; AY010609; AAG35368.1; -; Genomic_DNA.
DR EMBL; AY010610; AAG35369.1; -; Genomic_DNA.
DR EMBL; AY010611; AAG35370.1; -; Genomic_DNA.
DR EMBL; AY010612; AAG35371.1; -; Genomic_DNA.
DR EMBL; AY010613; AAG35372.1; -; Genomic_DNA.
DR EMBL; AY010614; AAG35373.1; -; Genomic_DNA.
DR EMBL; AY010615; AAG35374.1; -; Genomic_DNA.
DR EMBL; AY010616; AAG35375.1; -; Genomic_DNA.
DR EMBL; AY010617; AAG35376.1; -; Genomic_DNA.
DR RefSeq; NP_523892.1; NM_079168.3.
DR AlphaFoldDB; O46202; -.
DR BioGRID; 63842; 5.
DR DIP; DIP-20806N; -.
DR STRING; 7227.FBpp0072820; -.
DR MEROPS; I08.002; -.
DR PaxDb; O46202; -.
DR PRIDE; O46202; -.
DR DNASU; 38340; -.
DR EnsemblMetazoa; FBtr0072943; FBpp0072820; FBgn0020509.
DR GeneID; 38340; -.
DR KEGG; dme:Dmel_CG1262; -.
DR CTD; 38340; -.
DR FlyBase; FBgn0020509; Acp62F.
DR VEuPathDB; VectorBase:FBgn0020509; -.
DR HOGENOM; CLU_167788_0_0_1; -.
DR InParanoid; O46202; -.
DR OMA; RSDCPKD; -.
DR OrthoDB; 1625853at2759; -.
DR PhylomeDB; O46202; -.
DR BioGRID-ORCS; 38340; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 38340; -.
DR PRO; PR:O46202; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0020509; Expressed in male reproductive gland and 13 other tissues.
DR ExpressionAtlas; O46202; baseline and differential.
DR Genevisible; O46202; DM.
DR GO; GO:0005615; C:extracellular space; IDA:FlyBase.
DR GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IDA:FlyBase.
DR GO; GO:0045861; P:negative regulation of proteolysis; IDA:FlyBase.
DR GO; GO:0019953; P:sexual reproduction; HEP:FlyBase.
DR GO; GO:0046692; P:sperm competition; IMP:FlyBase.
DR InterPro; IPR036084; Ser_inhib-like_sf.
DR InterPro; IPR002919; TIL_dom.
DR Pfam; PF01826; TIL; 1.
DR SUPFAM; SSF57567; SSF57567; 1.
PE 1: Evidence at protein level;
KW Behavior; Reference proteome; Secreted; Signal.
FT SIGNAL 1..24
FT /evidence="ECO:0000255"
FT CHAIN 25..115
FT /note="Accessory gland protein Acp62F"
FT /id="PRO_0000034310"
FT DOMAIN 34..88
FT /note="TIL"
SQ SEQUENCE 115 AA; 12570 MW; 4326AA6F6C32291D CRC64;
MTDMWSLKIC ACLGLLLLFK PIDSMGWQGP KVDCTANGTQ TECPVACPET CEYSGNGPCV
KMCGAPCVCK PGYVINERIP ACVLRSDCPK DVVRKEDMLL GVSNFKCFSR NYNCS
