PIN2_YEAST
ID PIN2_YEAST Reviewed; 282 AA.
AC Q12057; D6W2G4;
DT 07-NOV-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=[PSI+] induction protein 2;
GN Name=PIN2; OrderedLocusNames=YOR104W; ORFNames=YOR3214W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=9200815;
RX DOI=10.1002/(sici)1097-0061(19970615)13:7<655::aid-yea120>3.0.co;2-i;
RA Voss H., Benes V., Andrade M.A., Valencia A., Rechmann S., Teodoru C.,
RA Schwager C., Paces V., Sander C., Ansorge W.;
RT "DNA sequencing and analysis of 130 kb from yeast chromosome XV.";
RL Yeast 13:655-672(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169874;
RA Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J.,
RA Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A.,
RA Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B.,
RA Dang V.-D., de Haan M., Delius H., Durand P., Fairhead C., Feldmann H.,
RA Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E.,
RA Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U.,
RA Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B.,
RA Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A.,
RA Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C.,
RA Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G.,
RA Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B.,
RA Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B.,
RA Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F.,
RA Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E.,
RA Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I.,
RA Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H.,
RA Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.";
RL Nature 387:98-102(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [5]
RP IDENTIFICATION, AND PRION FORMATION.
RX PubMed=11511345; DOI=10.1016/s0092-8674(01)00427-5;
RA Derkatch I.L., Bradley M.E., Hong J.Y., Liebman S.W.;
RT "Prions affect the appearance of other prions: the story of [PIN(+)].";
RL Cell 106:171-182(2001).
RN [6]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [7]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-141, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ADR376;
RX PubMed=17330950; DOI=10.1021/pr060559j;
RA Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J.,
RA Elias J.E., Gygi S.P.;
RT "Large-scale phosphorylation analysis of alpha-factor-arrested
RT Saccharomyces cerevisiae.";
RL J. Proteome Res. 6:1190-1197(2007).
CC -!- FUNCTION: Not known. Seems to be able to provoque the non-Mendelian
CC trait [PIN(+)] which is required for the de novo appearance of the
CC [PSI(+)] prion.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000269|PubMed:14562095}; Single-
CC pass membrane protein {ECO:0000269|PubMed:14562095}.
CC -!- MISCELLANEOUS: Present with 996 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; X94335; CAA64025.1; -; Genomic_DNA.
DR EMBL; Z75012; CAA99301.1; -; Genomic_DNA.
DR EMBL; AY557759; AAS56085.1; -; Genomic_DNA.
DR EMBL; BK006948; DAA10880.1; -; Genomic_DNA.
DR PIR; S61663; S61663.
DR RefSeq; NP_014747.1; NM_001183523.1.
DR AlphaFoldDB; Q12057; -.
DR SMR; Q12057; -.
DR BioGRID; 34501; 99.
DR DIP; DIP-4459N; -.
DR IntAct; Q12057; 14.
DR MINT; Q12057; -.
DR STRING; 4932.YOR104W; -.
DR iPTMnet; Q12057; -.
DR SwissPalm; Q12057; -.
DR MaxQB; Q12057; -.
DR PaxDb; Q12057; -.
DR PRIDE; Q12057; -.
DR EnsemblFungi; YOR104W_mRNA; YOR104W; YOR104W.
DR GeneID; 854271; -.
DR KEGG; sce:YOR104W; -.
DR SGD; S000005630; PIN2.
DR VEuPathDB; FungiDB:YOR104W; -.
DR eggNOG; ENOG502S5JV; Eukaryota.
DR HOGENOM; CLU_077184_0_0_1; -.
DR InParanoid; Q12057; -.
DR OMA; ACILVIW; -.
DR BioCyc; YEAST:G3O-33635-MON; -.
DR PRO; PR:Q12057; -.
DR Proteomes; UP000002311; Chromosome XV.
DR RNAct; Q12057; protein.
DR GO; GO:0005935; C:cellular bud neck; IDA:SGD.
DR GO; GO:0000329; C:fungal-type vacuole membrane; HDA:SGD.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:SGD.
DR InterPro; IPR037504; PSI_induc_2.
DR PANTHER; PTHR40018; PTHR40018; 1.
PE 1: Evidence at protein level;
KW Amyloid; Glycoprotein; Membrane; Phosphoprotein; Prion; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..282
FT /note="[PSI+] induction protein 2"
FT /id="PRO_0000058442"
FT TRANSMEM 43..63
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 153..183
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 246..282
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 153..176
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 141
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:17330950"
FT CARBOHYD 91
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 132
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 276
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 282 AA; 32084 MW; B60FC4E951D2DC28 CRC64;
MNVCKLKEIV PLFPRSSFTD GVVSTGKSFR SWDTCMDNKA CKIIAIVGIV LACILVIWLI
GGLLTCFRQG VTGIGQFICW CCRCSNDRNG NNTMPVNEGF SRVNMGVAPP STVIYQPIQQ
PESAYYRNDA KNDTFYDEVK TPSNEVYELE EDFDLEKQKE KTRKKQQKER NKEGRSPSRV
APLVYEEENF EGSSPQPQYD ARNSFIQNAA NTGSNNAHVA SQSPIFDISD YGENYYYDNN
NINNNLQGNS YNTPSSNHRS PYPTENYQSY QGYKPNQSDR YY
