PIN3A_ORYSJ
ID PIN3A_ORYSJ Reviewed; 618 AA.
AC Q5VP70; D5A7J0; Q0JKX2; Q5VP71;
DT 10-JAN-2006, integrated into UniProtKB/Swiss-Prot.
DT 07-DEC-2004, sequence version 1.
DT 03-AUG-2022, entry version 86.
DE RecName: Full=Auxin efflux carrier component 3a {ECO:0000305};
DE Short=OsPIN3a {ECO:0000303|PubMed:16085936};
DE AltName: Full=OSPIN10a {ECO:0000303|PubMed:19825657};
DE AltName: Full=OsPIN3t {ECO:0000303|PubMed:22882529};
GN Name=PIN3A {ECO:0000303|PubMed:16085936};
GN OrderedLocusNames=Os01g0643300 {ECO:0000312|EMBL:BAS73380.1},
GN LOC_Os01g45550 {ECO:0000305};
GN ORFNames=OsJ_02793 {ECO:0000312|EMBL:EEE55070.1},
GN P0510C12.26-1 {ECO:0000312|EMBL:BAD68754.1},
GN P0510C12.26-2 {ECO:0000312|EMBL:BAD68753.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12447438; DOI=10.1038/nature01184;
RA Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y.,
RA Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H.,
RA Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.,
RA Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M.,
RA Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T.,
RA Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S.,
RA Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H.,
RA Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y.,
RA Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S.,
RA Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y.,
RA Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S.,
RA Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H.,
RA Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.;
RT "The genome sequence and structure of rice chromosome 1.";
RL Nature 420:312-316(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [7]
RP IDENTIFICATION.
RX DOI=10.1016/j.plantsci.2010.02.018;
RA Miyashita Y., Takasugi T., Ito Y.;
RT "Identification and expression analysis of PIN genes in rice.";
RL Plant Sci. 178:424-428(2010).
RN [8]
RP NOMENCLATURE.
RX PubMed=16085936; DOI=10.1093/pcp/pci183;
RA Xu M., Zhu L., Shou H., Wu P.;
RT "A PIN1 family gene, OsPIN1, involved in auxin-dependent adventitious root
RT emergence and tillering in rice.";
RL Plant Cell Physiol. 46:1674-1681(2005).
RN [9]
RP TISSUE SPECIFICITY.
RX PubMed=19825657; DOI=10.1093/mp/ssp023;
RA Wang J.R., Hu H., Wang G.H., Li J., Chen J.Y., Wu P.;
RT "Expression of PIN genes in rice (Oryza sativa L.): tissue specificity and
RT regulation by hormones.";
RL Mol. Plant 2:823-831(2009).
RN [10]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=22882529; DOI=10.1111/j.1365-313x.2012.05121.x;
RA Zhang Q., Li J., Zhang W., Yan S., Wang R., Zhao J., Li Y., Qi Z., Sun Z.,
RA Zhu Z.;
RT "The putative auxin efflux carrier OsPIN3t is involved in the drought
RT stress response and drought tolerance.";
RL Plant J. 72:805-816(2012).
CC -!- FUNCTION: Acts as a component of the auxin efflux carrier. Involved in
CC the polar auxin transport which may regulate crown root development and
CC response to water stress. {ECO:0000269|PubMed:22882529}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:22882529};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q5VP70-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q5VP70-2; Sequence=VSP_017010;
CC -!- TISSUE SPECIFICITY: Expressed in coleoptiles, roots, vascular bundles
CC of leaves, shoots, lamina joints and vascular bundles of the lemma and
CC filament (PubMed:22882529). Expressed in stem bases, stems, leaves and
CC young panicles (PubMed:19825657). {ECO:0000269|PubMed:19825657,
CC ECO:0000269|PubMed:22882529}.
CC -!- INDUCTION: By auxin and osmotic stress. Down-regulated by 1-N-
CC naphthylphthalamic acid (NPA), an auxin efflux inhibitor.
CC {ECO:0000269|PubMed:22882529}.
CC -!- MISCELLANEOUS: Seedlings silencing PIN3A display abnormal crown root
CC development. Seedling over-expressing PIN3A exhibit better shoot
CC growth, longer roots and a greater number of adventitious roots than
CC the wild-type when grown on PEG-induced osmotic stress.
CC {ECO:0000269|PubMed:22882529}.
CC -!- SIMILARITY: Belongs to the auxin efflux carrier (TC 2.A.69.1) family.
CC {ECO:0000305}.
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DR EMBL; AP003725; BAD68753.1; -; Genomic_DNA.
DR EMBL; AP003725; BAD68754.1; -; Genomic_DNA.
DR EMBL; AP008207; BAF05606.1; -; Genomic_DNA.
DR EMBL; AP014957; BAS73380.1; -; Genomic_DNA.
DR EMBL; AP014957; BAS73381.1; -; Genomic_DNA.
DR EMBL; CM000138; EEE55070.1; -; Genomic_DNA.
DR EMBL; AK063976; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; AK101504; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; BR000832; FAA00681.1; -; Genomic_DNA.
DR RefSeq; XP_015625677.1; XM_015770191.1. [Q5VP70-1]
DR AlphaFoldDB; Q5VP70; -.
DR STRING; 4530.OS01T0643300-01; -.
DR PaxDb; Q5VP70; -.
DR PRIDE; Q5VP70; -.
DR EnsemblPlants; Os01t0643300-01; Os01t0643300-01; Os01g0643300. [Q5VP70-1]
DR EnsemblPlants; Os01t0643300-02; Os01t0643300-02; Os01g0643300. [Q5VP70-2]
DR GeneID; 4326565; -.
DR Gramene; Os01t0643300-01; Os01t0643300-01; Os01g0643300. [Q5VP70-1]
DR Gramene; Os01t0643300-02; Os01t0643300-02; Os01g0643300. [Q5VP70-2]
DR KEGG; osa:4326565; -.
DR eggNOG; ENOG502QRM7; Eukaryota.
DR InParanoid; Q5VP70; -.
DR OMA; GAGQHQM; -.
DR OrthoDB; 337723at2759; -.
DR PlantReactome; R-OSA-5608118; Auxin signalling.
DR PlantReactome; R-OSA-8858053; Polar auxin transport.
DR Proteomes; UP000000763; Chromosome 1.
DR Proteomes; UP000007752; Chromosome 1.
DR Proteomes; UP000059680; Chromosome 1.
DR Genevisible; Q5VP70; OS.
DR GO; GO:0009921; C:auxin efflux carrier complex; IDA:UniProtKB.
DR GO; GO:0005783; C:endoplasmic reticulum; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0010329; F:auxin efflux transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0010315; P:auxin export across the plasma membrane; IBA:GO_Central.
DR GO; GO:0010252; P:auxin homeostasis; IBA:GO_Central.
DR GO; GO:0009926; P:auxin polar transport; IMP:UniProtKB.
DR GO; GO:0009734; P:auxin-activated signaling pathway; IEA:UniProtKB-KW.
DR InterPro; IPR014024; Auxin_eff_plant.
DR InterPro; IPR004776; Mem_trans.
DR Pfam; PF03547; Mem_trans; 1.
DR TIGRFAMs; TIGR00946; 2a69; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Auxin signaling pathway; Cell membrane; Glycoprotein;
KW Membrane; Reference proteome; Stress response; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..618
FT /note="Auxin efflux carrier component 3a"
FT /id="PRO_0000123792"
FT TRANSMEM 7..27
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 39..59
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 71..90
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 105..125
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 135..155
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 479..499
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 503..523
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 540..560
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 564..584
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 598..618
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 281..312
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 281..296
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 226
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 369
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 391
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 394..422
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12869764"
FT /id="VSP_017010"
FT CONFLICT 154
FT /note="L -> H (in Ref. 6; AK101504)"
FT /evidence="ECO:0000305"
FT CONFLICT 330
FT /note="S -> Y (in Ref. 6; AK101504)"
FT /evidence="ECO:0000305"
FT CONFLICT 348
FT /note="Missing (in Ref. 6; AK063976)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 618 AA; 65255 MW; 0E8EDDE44E3BF2EE CRC64;
MISGHDFYTV MAAVVPLYVA MFLAYGSVRW WGIFTPDQCS GINRFVAIFA VPLLSFHFIS
TNDPYAMNLR FLAADTLQKL LVLAGLAAWS RLPSRTGAPR LDWSITLFSL STLPNTLVMG
IPLLIAMYGP YSGSLMVQIV VLQCIIWYTL MLFLFEFRAA RMLIADQFPD TAASIVSLHV
DPDVVSLEGG HAETEAEVAA DGRLHVTVRR SSVSRRSLLV TPRPSNLTGA EIYSLSSSRN
PTPRGSNFNH ADFFAMVGGG PPPPTPAAVR GSSFGASELY SLQSSRGPTP RQSNFDEHSA
RPPKPPATTT GALNHDAKEL HMFVWSSSAS PVSEVSGLPV FSGGGGGGAL DVGAKEIHMV
IPADLPQNNG SGKEHEEYGA VALGGGGGGE NFSFGGGKTV DGAEAVDEEA ALPDGLTKMG
SSSTAELHPK VVDVDGPNAG GGAAGAGQYQ MPPASVMTRL ILIMVWRKLI RNPNTYSSLL
GLAWSLVAFR WHVSMPAIVE KSISILSDAG LGMAMFSLGL FMALQPSIIA CGKSAAVVSM
AVRFLAGPAV MAAASIAIGL RGTLLHVAIV QAALPQGIVP FVFAKEYNVH PAILSTAVIF
GMLIALPITL LYYILLGL
