PIN6_ARATH
ID PIN6_ARATH Reviewed; 570 AA.
AC Q9SQH6; O49294;
DT 16-FEB-2004, integrated into UniProtKB/Swiss-Prot.
DT 16-FEB-2004, sequence version 2.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=Auxin efflux carrier component 6 {ECO:0000303|PubMed:15817418};
DE Short=AtPIN6 {ECO:0000303|PubMed:15817418};
GN Name=PIN6 {ECO:0000303|PubMed:15817418};
GN OrderedLocusNames=At1g77110 {ECO:0000312|Araport:AT1G77110};
GN ORFNames=F22K20.18 {ECO:0000312|EMBL:AAC00629.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Friml J., Wisniewska J., Palme K.;
RT "PIN gene family in Arabidopsis thaliana.";
RL Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=15817418; DOI=10.1016/j.tplants.2005.02.009;
RA Paponov I.A., Teale W.D., Trebar M., Blilou I., Palme K.;
RT "The PIN auxin efflux facilitators: evolutionary and functional
RT perspectives.";
RL Trends Plant Sci. 10:170-177(2005).
RN [5]
RP SUBCELLULAR LOCATION, AND FUNCTION.
RX PubMed=19506555; DOI=10.1038/nature08066;
RA Mravec J., Skupa P., Bailly A., Hoyerova K., Krecek P., Bielach A.,
RA Petrasek J., Zhang J., Gaykova V., Stierhof Y.D., Dobrev P.I.,
RA Schwarzerova K., Rolcik J., Seifertova D., Luschnig C., Benkova E.,
RA Zazimalova E., Geisler M., Friml J.;
RT "Subcellular homeostasis of phytohormone auxin is mediated by the ER-
RT localized PIN5 transporter.";
RL Nature 459:1136-1140(2009).
RN [6]
RP TISSUE SPECIFICITY.
RX PubMed=22540348; DOI=10.1111/j.1365-313x.2012.05037.x;
RA Dal Bosco C., Dovzhenko A., Liu X., Woerner N., Rensch T., Eismann M.,
RA Eimer S., Hegermann J., Paponov I.A., Ruperti B., Heberle-Bors E.,
RA Touraev A., Cohen J.D., Palme K.;
RT "The endoplasmic reticulum localized PIN8 is a pollen-specific auxin
RT carrier involved in intracellular auxin homeostasis.";
RL Plant J. 71:860-870(2012).
RN [7]
RP FUNCTION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=23551385; DOI=10.1111/tpj.12184;
RA Bender R.L., Fekete M.L., Klinkenberg P.M., Hampton M., Bauer B.,
RA Malecha M., Lindgren K., Maki J.A., Perera M.A., Nikolau B.J., Carter C.J.;
RT "PIN6 is required for nectary auxin response and short stamen
RT development.";
RL Plant J. 74:893-904(2013).
RN [8]
RP FUNCTION, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION, AND TISSUE
RP SPECIFICITY.
RX PubMed=23437008; DOI=10.1371/journal.pgen.1003294;
RA Sawchuk M.G., Edgar A., Scarpella E.;
RT "Patterning of leaf vein networks by convergent auxin transport pathways.";
RL PLoS Genet. 9:E1003294-E1003294(2013).
RN [9]
RP FUNCTION.
RX PubMed=24304505; DOI=10.4161/psb.27205;
RA Sawchuk M.G., Scarpella E.;
RT "Control of vein patterning by intracellular auxin transport.";
RL Plant Signal. Behav. 8:E27205-E27205(2013).
RN [10]
RP FUNCTION, TISSUE SPECIFICITY, INDUCTION BY AUXIN, DEVELOPMENTAL STAGE, AND
RP DISRUPTION PHENOTYPE.
RX PubMed=23922907; DOI=10.1371/journal.pone.0070069;
RA Cazzonelli C.I., Vanstraelen M., Simon S., Yin K., Carron-Arthur A.,
RA Nisar N., Tarle G., Cuttriss A.J., Searle I.R., Benkova E., Mathesius U.,
RA Masle J., Friml J., Pogson B.J.;
RT "Role of the Arabidopsis PIN6 auxin transporter in auxin homeostasis and
RT auxin-mediated development.";
RL PLoS ONE 8:E70069-E70069(2013).
RN [11]
RP INDUCTION, AND FUNCTION.
RX PubMed=24180465; DOI=10.1111/tpj.12373;
RA Chen Y., Aung K., Rolcik J., Walicki K., Friml J., Brandizzi F.;
RT "Inter-regulation of the unfolded protein response and auxin signaling.";
RL Plant J. 77:97-107(2014).
RN [12]
RP TISSUE SPECIFICITY.
RX PubMed=24487186; DOI=10.4161/psb.27898;
RA Nisar N., Cuttriss A.J., Pogson B.J., Cazzonelli C.I.;
RT "The promoter of the Arabidopsis PIN6 auxin transporter enabled strong
RT expression in the vasculature of roots, leaves, floral stems and
RT reproductive organs.";
RL Plant Signal. Behav. 9:E27898-E27898(2014).
RN [13]
RP TISSUE SPECIFICITY, AND FUNCTION.
RX PubMed=26560462; DOI=10.1186/s12915-015-0208-3;
RA Verna C., Sawchuk M.G., Linh N.M., Scarpella E.;
RT "Control of vein network topology by auxin transport.";
RL BMC Biol. 13:94-94(2015).
CC -!- FUNCTION: Component of the intracellular auxin-transport pathway
CC (PubMed:19506555). Regulates auxin transport and auxin homeostasis
CC (PubMed:23922907). Directly involved in the regulation of nectar
CC production (PubMed:23551385). Involved in unfolded protein response
CC (UPR) activation (PubMed:24180465). Involved in the control of vein
CC patterning (PubMed:23437008, PubMed:24304505). Redundantly with PIN8,
CC inhibits the vein-formation-promoting functions of PIN5
CC (PubMed:26560462). PIN5, PIN6, and PIN8 control vein network geometry,
CC but they are expressed in mutually exclusive domains of leaf vascular
CC cells (PubMed:26560462). {ECO:0000269|PubMed:19506555,
CC ECO:0000269|PubMed:23437008, ECO:0000269|PubMed:23922907,
CC ECO:0000269|PubMed:24180465, ECO:0000269|PubMed:26560462}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000269|PubMed:19506555, ECO:0000269|PubMed:23437008}; Multi-pass
CC membrane protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in the vasculature of the primary root,
CC cotyledons, floral stem, sepals and the main transmitting tract of the
CC reproductive silique (PubMed:23922907, PubMed:24487186). Expressed in
CC embryos, shoot meristem, root tip and lateral root meristems
CC (PubMed:23922907). Expressed in the nectaries and the floral organ
CC boundaries of the anthers (PubMed:23551385, PubMed:23922907). Detected
CC in pollen (PubMed:22540348). Expressed in broad subepidermal domains
CC that narrowed to sites of vein formation (PubMed:23437008). Expressed
CC in veins of mature leaves (PubMed:24487186, PubMed:26560462).
CC {ECO:0000269|PubMed:22540348, ECO:0000269|PubMed:23437008,
CC ECO:0000269|PubMed:23551385, ECO:0000269|PubMed:23922907,
CC ECO:0000269|PubMed:24487186, ECO:0000269|PubMed:26560462}.
CC -!- DEVELOPMENTAL STAGE: Expressed during vein formation (PubMed:23437008).
CC Decreased expression during seedling development (PubMed:23922907).
CC {ECO:0000269|PubMed:23437008, ECO:0000269|PubMed:23922907}.
CC -!- INDUCTION: Up-regulated by auxin (PubMed:23922907).Down-regulated by
CC endoplasmic reticulum stress treatment (PubMed:24180465).
CC {ECO:0000269|PubMed:23922907, ECO:0000269|PubMed:24180465}.
CC -!- DISRUPTION PHENOTYPE: Enhanced root growth and faster lateral root
CC development (PubMed:23922907). Altered floral morphology and nectar
CC secretion (PubMed:23551385). {ECO:0000269|PubMed:23551385,
CC ECO:0000269|PubMed:23922907}.
CC -!- SIMILARITY: Belongs to the auxin efflux carrier (TC 2.A.69.1) family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC00629.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF087819; AAD52696.1; -; mRNA.
DR EMBL; AC002291; AAC00629.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE35936.1; -; Genomic_DNA.
DR PIR; A96800; A96800.
DR RefSeq; NP_177836.1; NM_106361.4.
DR AlphaFoldDB; Q9SQH6; -.
DR BioGRID; 29265; 29.
DR IntAct; Q9SQH6; 29.
DR STRING; 3702.AT1G77110.1; -.
DR iPTMnet; Q9SQH6; -.
DR PaxDb; Q9SQH6; -.
DR PRIDE; Q9SQH6; -.
DR ProteomicsDB; 235021; -.
DR EnsemblPlants; AT1G77110.1; AT1G77110.1; AT1G77110.
DR GeneID; 844046; -.
DR Gramene; AT1G77110.1; AT1G77110.1; AT1G77110.
DR KEGG; ath:AT1G77110; -.
DR Araport; AT1G77110; -.
DR TAIR; locus:2025312; AT1G77110.
DR eggNOG; ENOG502QVQM; Eukaryota.
DR HOGENOM; CLU_019285_1_1_1; -.
DR InParanoid; Q9SQH6; -.
DR OrthoDB; 523438at2759; -.
DR PhylomeDB; Q9SQH6; -.
DR PRO; PR:Q9SQH6; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9SQH6; baseline and differential.
DR Genevisible; Q9SQH6; AT.
DR GO; GO:0005783; C:endoplasmic reticulum; IBA:GO_Central.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0010329; F:auxin efflux transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0010315; P:auxin export across the plasma membrane; IBA:GO_Central.
DR GO; GO:0010252; P:auxin homeostasis; IBA:GO_Central.
DR GO; GO:0009926; P:auxin polar transport; IMP:TAIR.
DR GO; GO:0009734; P:auxin-activated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0010540; P:basipetal auxin transport; IMP:CACAO.
DR GO; GO:0010105; P:negative regulation of ethylene-activated signaling pathway; IMP:CACAO.
DR GO; GO:1901332; P:negative regulation of lateral root development; IMP:CACAO.
DR GO; GO:0048767; P:root hair elongation; IMP:CACAO.
DR InterPro; IPR014024; Auxin_eff_plant.
DR InterPro; IPR004776; Mem_trans.
DR Pfam; PF03547; Mem_trans; 1.
DR TIGRFAMs; TIGR00946; 2a69; 1.
PE 2: Evidence at transcript level;
KW Auxin signaling pathway; Endoplasmic reticulum; Glycoprotein; Membrane;
KW Phosphoprotein; Reference proteome; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..570
FT /note="Auxin efflux carrier component 6"
FT /id="PRO_0000123785"
FT TRANSMEM 7..27
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 39..59
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 71..91
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 101..121
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 132..152
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 431..451
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 455..475
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 492..512
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 516..536
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 550..570
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MOD_RES 230
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9C6B8"
FT MOD_RES 308
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9C6B8"
FT CARBOHYD 231
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 221
FT /note="S -> P (in Ref. 1; AAD52696)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 570 AA; 62044 MW; 73611B0C4E2DF4F3 CRC64;
MITGNEFYTV MCAMAPLYFA MFVAYGSVKW CKIFTPAQCS GINRFVSVFA VPVLSFHFIS
QNNPYKMDTM FILADTLSKI FVFVLLSLWA VFFKAGGLDW LITLFSIATL PNTLVMGIPL
LQAMYGDYTQ TLMVQLVVLQ CIIWYTLLLF LFELRAARLL IRAEFPGQAA GSIAKIQVDD
DVISLDGMDP LRTETETDVN GRIRLRIRRS VSSVPDSVMS SSLCLTPRAS NLSNAEIFSV
NTPNNRFFHG GGGSGTLQFY NGSNEIMFCN GDLGGFGFTR PGLGASPRRL SGYASSDAYS
LQPTPRASNF NELDVNGNGT PVWMKSPAAG RIYRQSSPKM MWESGQRHAA KDINGSVPEK
EISFRDALKA APQATAAGGG ASMEEGAAGK DTTPVAAIGK QEMPSAIVMM RLILTVVGRK
LSRNPNTYSS LLGLVWSLIS FKWNIPMPNI VDFSIKIISD AGLGMAMFSL GLFMALQPKM
IPCGAKKATM GMLIRFISGP LFMAGASLLV GLRGSRLHAA IVQAALPQGI VPFVFAREYN
LHPDLLSTLV IFGMIVSLPV TILYYVLLGL
