PIN8_ARATH
ID PIN8_ARATH Reviewed; 367 AA.
AC Q9LFP6;
DT 16-FEB-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=Auxin efflux carrier component 8 {ECO:0000303|PubMed:15817418};
DE Short=AtPIN8 {ECO:0000303|PubMed:15817418};
GN Name=PIN8 {ECO:0000303|PubMed:15817418}; Synonyms=PIN5;
GN OrderedLocusNames=At5g15100 {ECO:0000312|Araport:AT5G15100};
GN ORFNames=F2G14_220 {ECO:0000312|EMBL:CAC01829.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=15817418; DOI=10.1016/j.tplants.2005.02.009;
RA Paponov I.A., Teale W.D., Trebar M., Blilou I., Palme K.;
RT "The PIN auxin efflux facilitators: evolutionary and functional
RT perspectives.";
RL Trends Plant Sci. 10:170-177(2005).
RN [4]
RP SUBCELLULAR LOCATION.
RX PubMed=19506555; DOI=10.1038/nature08066;
RA Mravec J., Skupa P., Bailly A., Hoyerova K., Krecek P., Bielach A.,
RA Petrasek J., Zhang J., Gaykova V., Stierhof Y.D., Dobrev P.I.,
RA Schwarzerova K., Rolcik J., Seifertova D., Luschnig C., Benkova E.,
RA Zazimalova E., Geisler M., Friml J.;
RT "Subcellular homeostasis of phytohormone auxin is mediated by the ER-
RT localized PIN5 transporter.";
RL Nature 459:1136-1140(2009).
RN [5]
RP SUBCELLULAR LOCATION, AND FUNCTION.
RX PubMed=20439545; DOI=10.1104/pp.110.156505;
RA Ganguly A., Lee S.H., Cho M., Lee O.R., Yoo H., Cho H.T.;
RT "Differential auxin-transporting activities of PIN-FORMED proteins in
RT Arabidopsis root hair cells.";
RL Plant Physiol. 153:1046-1061(2010).
RN [6]
RP FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE, AND SUBCELLULAR
RP LOCATION.
RX PubMed=22760640; DOI=10.1038/ncomms1941;
RA Ding Z., Wang B., Moreno I., Duplakova N., Simon S., Carraro N.,
RA Reemmer J., Pencik A., Chen X., Tejos R., Skupa P., Pollmann S., Mravec J.,
RA Petrasek J., Zazimalova E., Honys D., Rolcik J., Murphy A., Orellana A.,
RA Geisler M., Friml J.;
RT "ER-localized auxin transporter PIN8 regulates auxin homeostasis and male
RT gametophyte development in Arabidopsis.";
RL Nat. Commun. 3:941-941(2012).
RN [7]
RP FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION,
RP AND DISRUPTION PHENOTYPE.
RX PubMed=22540348; DOI=10.1111/j.1365-313x.2012.05037.x;
RA Dal Bosco C., Dovzhenko A., Liu X., Woerner N., Rensch T., Eismann M.,
RA Eimer S., Hegermann J., Paponov I.A., Ruperti B., Heberle-Bors E.,
RA Touraev A., Cohen J.D., Palme K.;
RT "The endoplasmic reticulum localized PIN8 is a pollen-specific auxin
RT carrier involved in intracellular auxin homeostasis.";
RL Plant J. 71:860-870(2012).
RN [8]
RP FUNCTION.
RX PubMed=22990451; DOI=10.4161/psb.21953;
RA Dal Bosco C., Dovzhenko A., Palme K.;
RT "Intracellular auxin transport in pollen: PIN8, PIN5 and PILS5.";
RL Plant Signal. Behav. 7:1504-1505(2012).
RN [9]
RP FUNCTION, DEVELOPMENTAL STAGE, AND SUBCELLULAR LOCATION.
RX PubMed=23437008; DOI=10.1371/journal.pgen.1003294;
RA Sawchuk M.G., Edgar A., Scarpella E.;
RT "Patterning of leaf vein networks by convergent auxin transport pathways.";
RL PLoS Genet. 9:E1003294-E1003294(2013).
RN [10]
RP FUNCTION.
RX PubMed=24304505; DOI=10.4161/psb.27205;
RA Sawchuk M.G., Scarpella E.;
RT "Control of vein patterning by intracellular auxin transport.";
RL Plant Signal. Behav. 8:E27205-E27205(2013).
RN [11]
RP TISSUE SPECIFICITY, AND FUNCTION.
RX PubMed=26560462; DOI=10.1186/s12915-015-0208-3;
RA Verna C., Sawchuk M.G., Linh N.M., Scarpella E.;
RT "Control of vein network topology by auxin transport.";
RL BMC Biol. 13:94-94(2015).
CC -!- FUNCTION: Component of the intracellular auxin-transport pathway in the
CC male gametophyte (PubMed:20439545, PubMed:22540348). Involved in the
CC regulation of auxin homeostasis in pollen (PubMed:22540348). Involved
CC in the efflux of auxin from the endoplasmic reticulum into the
CC cytoplasm (PubMed:22760640). PIN5 and PIN8 may have an
CC antagonistic/compensatory activity (PubMed:22760640, PubMed:22990451).
CC Involved in the control of vein patterning (PubMed:23437008,
CC PubMed:24304505). Redundantly with PIN6, inhibits the vein-formation-
CC promoting functions of PIN5 (PubMed:26560462). PIN5, PIN6, and PIN8
CC control vein network geometry, but they are expressed in mutually
CC exclusive domains of leaf vascular cells (PubMed:26560462).
CC {ECO:0000269|PubMed:20439545, ECO:0000269|PubMed:22540348,
CC ECO:0000269|PubMed:22760640, ECO:0000269|PubMed:23437008,
CC ECO:0000269|PubMed:24304505, ECO:0000269|PubMed:26560462,
CC ECO:0000305|PubMed:22990451}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000269|PubMed:19506555, ECO:0000269|PubMed:20439545,
CC ECO:0000269|PubMed:22540348, ECO:0000269|PubMed:22760640,
CC ECO:0000269|PubMed:23437008}; Multi-pass membrane protein
CC {ECO:0000305}. Cell membrane {ECO:0000269|PubMed:20439545}; Multi-pass
CC membrane protein {ECO:0000305}. Note=Localizes to endoplasmic reticulum
CC in pollen, growing pollen tubes and leaves.
CC {ECO:0000269|PubMed:22540348, ECO:0000269|PubMed:22760640}.
CC -!- TISSUE SPECIFICITY: Expressed in veins of mature leaves
CC (PubMed:26560462). Strongly expressed in pollen (PubMed:22760640,
CC PubMed:22540348). {ECO:0000269|PubMed:22540348,
CC ECO:0000269|PubMed:22760640, ECO:0000269|PubMed:26560462}.
CC -!- DEVELOPMENTAL STAGE: Expressed at later stages of microgametogenesis,
CC after the pollen has reached the tricellular stage and until pollen
CC dehiscence (PubMed:22540348). Expressed during vein formation
CC (PubMed:23437008). {ECO:0000269|PubMed:22540348,
CC ECO:0000269|PubMed:23437008}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype (PubMed:22760640,
CC PubMed:22540348). Increased frequency of aborted and misshaped pollen
CC grains and decreased pollen germination (PubMed:22760640).
CC {ECO:0000269|PubMed:22540348, ECO:0000269|PubMed:22760640}.
CC -!- SIMILARITY: Belongs to the auxin efflux carrier (TC 2.A.69.1) family.
CC {ECO:0000305}.
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DR EMBL; AL391146; CAC01829.1; -; Genomic_DNA.
DR EMBL; CP002688; AED92117.1; -; Genomic_DNA.
DR PIR; T51455; T51455.
DR RefSeq; NP_197014.1; NM_121514.2.
DR AlphaFoldDB; Q9LFP6; -.
DR STRING; 3702.AT5G15100.1; -.
DR TCDB; 2.A.69.1.7; the auxin efflux carrier (aec) family.
DR PaxDb; Q9LFP6; -.
DR PRIDE; Q9LFP6; -.
DR ProteomicsDB; 236756; -.
DR EnsemblPlants; AT5G15100.1; AT5G15100.1; AT5G15100.
DR GeneID; 831362; -.
DR Gramene; AT5G15100.1; AT5G15100.1; AT5G15100.
DR KEGG; ath:AT5G15100; -.
DR Araport; AT5G15100; -.
DR TAIR; locus:2147835; AT5G15100.
DR eggNOG; ENOG502QS5F; Eukaryota.
DR HOGENOM; CLU_019285_0_0_1; -.
DR InParanoid; Q9LFP6; -.
DR OMA; MIASAYC; -.
DR OrthoDB; 1106458at2759; -.
DR PhylomeDB; Q9LFP6; -.
DR PRO; PR:Q9LFP6; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9LFP6; baseline and differential.
DR Genevisible; Q9LFP6; AT.
DR GO; GO:0005783; C:endoplasmic reticulum; IDA:TAIR.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0010329; F:auxin efflux transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0010315; P:auxin export across the plasma membrane; IMP:TAIR.
DR GO; GO:0010252; P:auxin homeostasis; IMP:TAIR.
DR GO; GO:0009926; P:auxin polar transport; IBA:GO_Central.
DR GO; GO:0009734; P:auxin-activated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0009555; P:pollen development; IMP:TAIR.
DR InterPro; IPR014024; Auxin_eff_plant.
DR InterPro; IPR004776; Mem_trans.
DR Pfam; PF03547; Mem_trans; 1.
DR TIGRFAMs; TIGR00946; 2a69; 1.
PE 2: Evidence at transcript level;
KW Auxin signaling pathway; Cell membrane; Endoplasmic reticulum; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..367
FT /note="Auxin efflux carrier component 8"
FT /id="PRO_0000123784"
FT TRANSMEM 7..27
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 39..59
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 70..90
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 106..126
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 137..157
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 228..248
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 252..272
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 289..309
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 313..333
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 345..365
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 168..194
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 367 AA; 40502 MW; 1CC05A4716563E25 CRC64;
MISWLDIYHV VSATVPLYVS MTLGFLSARH LKLFSPEQCA GINKFVAKFS IPLLSFQIIS
ENNPFKMSPK LILSDILQKF LVVVVLAMVL RFWHPTGGRG GKLGWVITGL SISVLPNTLI
LGMPILSAIY GDEAASILEQ IVVLQSLIWY TILLFLFELN AARALPSSGA SLEHTGNDQE
EANIEDEPKE EEDEEEVAIV RTRSVGTMKI LLKAWRKLII NPNTYATLIG IIWATLHFRL
GWNLPEMIDK SIHLLSDGGL GMAMFSLGLF MASQSSIIAC GTKMAIITML LKFVLGPALM
IASAYCIRLK STLFKVAILQ AALPQGVVPF VFAKEYNLHP EIISTGVIFG MLIALPTTLA
YYFLLDL
