PINE_ECOLI
ID PINE_ECOLI Reviewed; 184 AA.
AC P03014;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 2.
DT 03-AUG-2022, entry version 155.
DE RecName: Full=Serine recombinase PinE {ECO:0000250|UniProtKB:P03015};
DE EC=3.1.22.-;
DE EC=6.5.1.-;
DE AltName: Full=DNA-invertase PinE {ECO:0000305};
DE Short=DNA-invertase from lambdoid prophage e14;
DE AltName: Full=Site-specific recombinase PinE {ECO:0000305};
GN Name=pinE; Synonyms=pin; OrderedLocusNames=b1158, JW1144;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=3894006; DOI=10.1002/j.1460-2075.1985.tb02341.x;
RA Plasterk R.H.A., van de Putte P.;
RT "The invertible P-DNA segment in the chromosome of Escherichia coli.";
RL EMBO J. 4:237-242(1985).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=6310572; DOI=10.1073/pnas.80.17.5355;
RA Plasterk R.H.A., Brinkman A., van de Putte P.;
RT "DNA inversions in the chromosome of Escherichia coli and in bacteriophage
RT Mu: relationship to other site-specific recombination systems.";
RL Proc. Natl. Acad. Sci. U.S.A. 80:5355-5358(1983).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=8905232; DOI=10.1093/dnares/3.3.137;
RA Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K.,
RA Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S.,
RA Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H.,
RA Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G.,
RA Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M.,
RA Horiuchi T.;
RT "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 12.7-28.0 min region on the linkage map.";
RL DNA Res. 3:137-155(1996).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
CC -!- FUNCTION: This protein catalyzes the inversion of an 1800-bp E.coli DNA
CC fragment, the P region, which can exist in either orientation. The
CC function of the inversion is not yet clear.
CC -!- SIMILARITY: Belongs to the site-specific recombinase resolvase family.
CC {ECO:0000305}.
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DR EMBL; K00676; AAA24391.1; -; Genomic_DNA.
DR EMBL; X01805; CAA25948.1; -; Genomic_DNA.
DR EMBL; U00096; AAC74242.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA35994.1; -; Genomic_DNA.
DR PIR; A03545; JWEC.
DR RefSeq; NP_415676.1; NC_000913.3.
DR RefSeq; WP_000905001.1; NZ_CP047127.1.
DR AlphaFoldDB; P03014; -.
DR SMR; P03014; -.
DR BioGRID; 4262859; 144.
DR BioGRID; 850091; 1.
DR IntAct; P03014; 2.
DR STRING; 511145.b1158; -.
DR PaxDb; P03014; -.
DR PRIDE; P03014; -.
DR EnsemblBacteria; AAC74242; AAC74242; b1158.
DR EnsemblBacteria; BAA35994; BAA35994; BAA35994.
DR GeneID; 945721; -.
DR KEGG; ecj:JW1144; -.
DR KEGG; eco:b1158; -.
DR PATRIC; fig|511145.12.peg.1199; -.
DR EchoBASE; EB0730; -.
DR eggNOG; COG1961; Bacteria.
DR HOGENOM; CLU_010686_8_0_6; -.
DR InParanoid; P03014; -.
DR OMA; MALKMYD; -.
DR PhylomeDB; P03014; -.
DR BioCyc; EcoCyc:EG10737-MON; -.
DR BioCyc; MetaCyc:EG10737-MON; -.
DR PRO; PR:P03014; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000150; F:DNA strand exchange activity; IMP:EcoCyc.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0016874; F:ligase activity; IEA:UniProtKB-KW.
DR GO; GO:0015074; P:DNA integration; IEA:UniProtKB-KW.
DR GO; GO:0006310; P:DNA recombination; IMP:EcoCyc.
DR Gene3D; 3.40.50.1390; -; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR006118; Recombinase_CS.
DR InterPro; IPR006119; Resolv_N.
DR InterPro; IPR036162; Resolvase-like_N_sf.
DR InterPro; IPR006120; Resolvase_HTH_dom.
DR Pfam; PF02796; HTH_7; 1.
DR Pfam; PF00239; Resolvase; 1.
DR SMART; SM00857; Resolvase; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR SUPFAM; SSF53041; SSF53041; 1.
DR PROSITE; PS00397; RECOMBINASES_1; 1.
DR PROSITE; PS00398; RECOMBINASES_2; 1.
DR PROSITE; PS51736; RECOMBINASES_3; 1.
PE 3: Inferred from homology;
KW DNA integration; DNA invertase; DNA recombination; DNA-binding; Hydrolase;
KW Ligase; Reference proteome.
FT CHAIN 1..184
FT /note="Serine recombinase PinE"
FT /id="PRO_0000196360"
FT DOMAIN 1..134
FT /note="Resolvase/invertase-type recombinase catalytic"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01072"
FT DNA_BIND 161..180
FT /note="H-T-H motif"
FT /evidence="ECO:0000255"
FT ACT_SITE 9
FT /note="O-(5'-phospho-DNA)-serine intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01072"
FT CONFLICT 178
FT /note="R -> T (in Ref. 1; CAA25948)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 184 AA; 20573 MW; 5CD5F23F1E21A1DE CRC64;
MLIGYVRVST NDQNTDLQRN ALNCAGCELI FEDKISGTKS ERPGLKKLLR TLSAGDTLVV
WKLDRLGRSM RHLVVLVEEL RERGINFRSL TDSIDTSTPM GRFFFHVMGA LAEMERELIV
ERTKAGLETA RAQGRIGGRR PKLTPEQWAQ AGRLIAAGTP RQKVAIIYDV GVSTLYKRFP
AGDK
