ID   PINQ_ECOLI              Reviewed;         196 AA.
AC   P77170;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   03-AUG-2022, entry version 140.
DE   RecName: Full=Serine recombinase PinQ {ECO:0000250|UniProtKB:P03015};
DE            EC=3.1.22.-;
DE            EC=6.5.1.-;
DE   AltName: Full=DNA-invertase PinQ {ECO:0000305};
DE   AltName: Full=Putative DNA-invertase from lambdoid prophage Qin;
DE   AltName: Full=Site-specific recombinase PinQ {ECO:0000305};
GN   Name=pinQ; Synonyms=ydfL; OrderedLocusNames=b1545, JW1538;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=9097039; DOI=10.1093/dnares/3.6.363;
RA   Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T.,
RA   Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K.,
RA   Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S.,
RA   Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G.,
RA   Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y.,
RA   Wada C., Yamamoto Y., Horiuchi T.;
RT   "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT   the 28.0-40.1 min region on the linkage map.";
RL   DNA Res. 3:363-377(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
CC   -!- SIMILARITY: Belongs to the site-specific recombinase resolvase family.
CC       {ECO:0000305}.
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DR   EMBL; U00096; AAC74618.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAA15249.1; -; Genomic_DNA.
DR   PIR; D64909; D64909.
DR   RefSeq; NP_416063.1; NC_000913.3.
DR   RefSeq; WP_000078177.1; NZ_LN832404.1.
DR   AlphaFoldDB; P77170; -.
DR   SMR; P77170; -.
DR   BioGRID; 4260217; 202.
DR   DIP; DIP-10508N; -.
DR   IntAct; P77170; 1.
DR   STRING; 511145.b1545; -.
DR   PaxDb; P77170; -.
DR   PRIDE; P77170; -.
DR   EnsemblBacteria; AAC74618; AAC74618; b1545.
DR   EnsemblBacteria; BAA15249; BAA15249; BAA15249.
DR   GeneID; 946088; -.
DR   KEGG; ecj:JW1538; -.
DR   KEGG; eco:b1545; -.
DR   PATRIC; fig|511145.12.peg.1615; -.
DR   EchoBASE; EB3585; -.
DR   eggNOG; COG1961; Bacteria.
DR   HOGENOM; CLU_010686_8_2_6; -.
DR   InParanoid; P77170; -.
DR   OMA; QRQMEGI; -.
DR   PhylomeDB; P77170; -.
DR   BioCyc; EcoCyc:G6819-MON; -.
DR   PRO; PR:P77170; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000150; F:DNA strand exchange activity; IBA:GO_Central.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016874; F:ligase activity; IEA:UniProtKB-KW.
DR   GO; GO:0015074; P:DNA integration; IEA:UniProtKB-KW.
DR   GO; GO:0006310; P:DNA recombination; IBA:GO_Central.
DR   Gene3D; 3.40.50.1390; -; 1.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR006118; Recombinase_CS.
DR   InterPro; IPR006119; Resolv_N.
DR   InterPro; IPR036162; Resolvase-like_N_sf.
DR   InterPro; IPR006120; Resolvase_HTH_dom.
DR   Pfam; PF02796; HTH_7; 1.
DR   Pfam; PF00239; Resolvase; 1.
DR   SMART; SM00857; Resolvase; 1.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   SUPFAM; SSF53041; SSF53041; 1.
DR   PROSITE; PS00398; RECOMBINASES_2; 1.
DR   PROSITE; PS51736; RECOMBINASES_3; 1.
PE   3: Inferred from homology;
KW   DNA integration; DNA invertase; DNA recombination; DNA-binding; Hydrolase;
KW   Ligase; Reference proteome.
FT   CHAIN           1..196
FT                   /note="Serine recombinase PinQ"
FT                   /id="PRO_0000196362"
FT   DOMAIN          3..143
FT                   /note="Resolvase/invertase-type recombinase catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01072"
FT   ACT_SITE        11
FT                   /note="O-(5'-phospho-DNA)-serine intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01072"
SQ   SEQUENCE   196 AA;  21852 MW;  7CF47F6D88DBD497 CRC64;
     MSQIFAYCRI STLDQTTENQ RREIESAGFK IKPQQIIEEH ISGSAATSER PGFNRLLARL
     KCGDQLIVTK LDRLGCNAMD IRKTVEQLTE TGIRVHCLAL GGIDLTSPTG KMMMQVISAV
     AEFERDLLLE RTHSGIVRAR GAGKRFGRPP VLNEEQKQAV FERIKSGVSI SAIAREFKTS
     RQTILRAKAK LQTPDI