ID   PIP12_MUSAC             Reviewed;         258 AA.
AC   P0DO53;
DT   03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT   03-AUG-2022, sequence version 1.
DT   03-AUG-2022, entry version 1.
DE   RecName: Full=Aquaporin PIP1-2 {ECO:0000303|PubMed:23745761, ECO:0000303|PubMed:26307965};
DE   AltName: Full=Plasma membrane intrinsic protein 1-2 {ECO:0000303|PubMed:23745761, ECO:0000303|PubMed:26307965};
DE            Short=MusaPIP1-2 {ECO:0000303|PubMed:26307965};
DE            Short=MusaPIP1;2 {ECO:0000303|PubMed:23745761};
DE   Flags: Fragment;
GN   Name=PIP1-2 {ECO:0000303|PubMed:23745761, ECO:0000303|PubMed:26307965};
OS   Musa acuminata (Banana) (Musa cavendishii).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Zingiberales; Musaceae; Musa.
OX   NCBI_TaxID=4641;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Mpologoma (AAA), cv. Pisang Awak (ABB), and
RC   cv. Sukari Ndizi (AB); TISSUE=Root;
RA   Hearne S.J., Town C.D., Moskal W.A. Jr., Zhuang J., Viswanathan L.D.,
RA   Kriga Y., Shatsman S., Shetty J., Ferguson M.E.;
RT   "ESTs from drought stressed and non-stressed leaves, shoot meristem and
RT   roots of drought tolerant and drought sensitive Musa cultivars.";
RL   Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   TISSUE SPECIFICITY, GENE FAMILY, AND NOMENCLATURE.
RC   STRAIN=cv. Cavendish (AAA);
RX   PubMed=26307965; DOI=10.3390/ijms160819728;
RA   Hu W., Hou X., Huang C., Yan Y., Tie W., Ding Z., Wei Y., Liu J., Miao H.,
RA   Lu Z., Li M., Xu B., Jin Z.;
RT   "Genome-wide identification and expression analyses of aquaporin gene
RT   family during development and abiotic stress in banana.";
RL   Int. J. Mol. Sci. 16:19728-19751(2015).
RN   [3]
RP   FUNCTION, SUBCELLULAR LOCATION, AND BIOTECHNOLOGY.
RC   STRAIN=cv. Karibale Monthan, and cv. Rasthali;
RX   PubMed=23745761; DOI=10.1111/pbi.12086;
RA   Sreedharan S., Shekhawat U.K.S., Ganapathi T.R.;
RT   "Transgenic banana plants overexpressing a native plasma membrane aquaporin
RT   MusaPIP1;2 display high tolerance levels to different abiotic stresses.";
RL   Plant Biotechnol. J. 11:942-952(2013).
CC   -!- FUNCTION: Water channel required to facilitate the transport of water
CC       across cell membrane; mercury-insensitive (By similarity). Contributes
CC       to the tolerance to multiple abiotic stresses including salt (NaCl),
CC       cold and water deprivation, by modulating cytosolic K(+)/Na(+) ratio,
CC       maintaining osmotic balance, and reducing membrane injury (e.g.
CC       oxidative injury) (PubMed:23745761). {ECO:0000250|UniProtKB:P30302,
CC       ECO:0000269|PubMed:23745761}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:23745761};
CC       Multi-pass membrane protein {ECO:0000255}.
CC   -!- TISSUE SPECIFICITY: Barely detectable in roots, leaves and fruits.
CC       {ECO:0000269|PubMed:26307965}.
CC   -!- DOMAIN: Aquaporins contain two tandem repeats each containing three
CC       membrane-spanning domains and a pore-forming loop with the signature
CC       motif Asn-Pro-Ala (NPA). {ECO:0000305}.
CC   -!- BIOTECHNOLOGY: Can be used to improve resistance to abiotic stresses
CC       such as cold, drought and salt. {ECO:0000269|PubMed:23745761}.
CC   -!- SIMILARITY: Belongs to the MIP/aquaporin (TC 1.A.8) family. PIP (TC
CC       1.A.8.11) subfamily. {ECO:0000305}.
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DR   EMBL; FF561783; -; NOT_ANNOTATED_CDS; mRNA.
DR   PROSITE; PS00221; MIP; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Membrane; Stress response; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..>258
FT                   /note="Aquaporin PIP1-2"
FT                   /id="PRO_0000455807"
FT   TOPO_DOM        1..55
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        56..76
FT                   /note="Helical; Name=1"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        77..89
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        90..110
FT                   /note="Helical; Name=2"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        111..133
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        134..154
FT                   /note="Helical; Name=3"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        155..175
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        176..196
FT                   /note="Helical; Name=4"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        197..209
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        210..230
FT                   /note="Helical; Name=5"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        231..258
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000305"
FT   MOTIF           115..117
FT                   /note="NPA 1"
FT                   /evidence="ECO:0000255"
FT   MOTIF           236..238
FT                   /note="NPA 2"
FT                   /evidence="ECO:0000255"
FT   NON_TER         258
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   258 AA;  27431 MW;  57681EFCBABDE89C CRC64;
     MEGKEEDVRL GANKFTERQP IGTAAQSQDK DYKEPPPAPL FEPGELSSWS FYRAGIAEFV
     ATFLFLYITI LTVMGVVKSS TKCSTVGIQG IAWAFGGMIF ALVYCTAGIS GGHINPAVTF
     GLFLARKLSL TRALFYMVMQ CLGAICGAGV VKGFQKGLYE NNGGGANVVA PGYTKGDGLG
     AEIVGTFILV YTVFSATDAK RSARDSHVPI LAPLPIGFAV FLVHLATIPI TGTGINPARS
     LGAAIIYNKG HAWDDHWI