ID   PIP1_SOLLC              Reviewed;         286 AA.
AC   Q08451;
DT   01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1994, sequence version 1.
DT   03-AUG-2022, entry version 119.
DE   RecName: Full=Probable aquaporin PIP-type pTOM75;
DE   AltName: Full=Ripening-associated membrane protein;
DE            Short=RAMP;
OS   Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum;
OC   Solanum subgen. Lycopersicon.
OX   NCBI_TaxID=4081;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=7510135; DOI=10.1007/bf00024122;
RA   Fray R.G., Wallace A., Grierson D., Lycett G.W.;
RT   "Nucleotide sequence and expression of a ripening and water stress-related
RT   cDNA from tomato with homology to the MIP class of membrane channel
RT   proteins.";
RL   Plant Mol. Biol. 24:539-543(1994).
CC   -!- FUNCTION: Aquaporins facilitate the transport of water and small
CC       neutral solutes across cell membranes. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC       protein {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Roots, ripening fruit and senescing leaves.
CC   -!- INDUCTION: By water stress.
CC   -!- DOMAIN: Aquaporins contain two tandem repeats each containing three
CC       membrane-spanning domains and a pore-forming loop with the signature
CC       motif Asn-Pro-Ala (NPA).
CC   -!- SIMILARITY: Belongs to the MIP/aquaporin (TC 1.A.8) family. PIP (TC
CC       1.A.8.11) subfamily. {ECO:0000305}.
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DR   EMBL; X73848; CAA52068.1; -; mRNA.
DR   EMBL; X73847; CAA52067.1; -; mRNA.
DR   PIR; S42542; S42542.
DR   RefSeq; NP_001234139.1; NM_001247210.2.
DR   AlphaFoldDB; Q08451; -.
DR   SMR; Q08451; -.
DR   STRING; 4081.Solyc08g081190.2.1; -.
DR   PaxDb; Q08451; -.
DR   PRIDE; Q08451; -.
DR   EnsemblPlants; Solyc08g081190.3.1; Solyc08g081190.3.1; Solyc08g081190.3.
DR   GeneID; 544086; -.
DR   Gramene; Solyc08g081190.3.1; Solyc08g081190.3.1; Solyc08g081190.3.
DR   KEGG; sly:544086; -.
DR   eggNOG; KOG0223; Eukaryota.
DR   HOGENOM; CLU_020019_3_0_1; -.
DR   InParanoid; Q08451; -.
DR   OMA; EGGNQYK; -.
DR   OrthoDB; 1152704at2759; -.
DR   PhylomeDB; Q08451; -.
DR   Proteomes; UP000004994; Chromosome 8.
DR   ExpressionAtlas; Q08451; baseline and differential.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR   GO; GO:0015250; F:water channel activity; IBA:GO_Central.
DR   GO; GO:0009414; P:response to water deprivation; IBA:GO_Central.
DR   CDD; cd00333; MIP; 1.
DR   Gene3D; 1.20.1080.10; -; 1.
DR   InterPro; IPR023271; Aquaporin-like.
DR   InterPro; IPR034294; Aquaporin_transptr.
DR   InterPro; IPR000425; MIP.
DR   InterPro; IPR022357; MIP_CS.
DR   PANTHER; PTHR45687; PTHR45687; 1.
DR   Pfam; PF00230; MIP; 1.
DR   PRINTS; PR00783; MINTRINSICP.
DR   SUPFAM; SSF81338; SSF81338; 1.
DR   TIGRFAMs; TIGR00861; MIP; 1.
DR   PROSITE; PS00221; MIP; 1.
PE   2: Evidence at transcript level;
KW   Cell membrane; Membrane; Reference proteome; Repeat; Stress response;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..286
FT                   /note="Probable aquaporin PIP-type pTOM75"
FT                   /id="PRO_0000064059"
FT   TOPO_DOM        1..55
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        56..76
FT                   /note="Helical; Name=1"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        77..89
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        90..110
FT                   /note="Helical; Name=2"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        111..133
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        134..154
FT                   /note="Helical; Name=3"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        155..175
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        176..196
FT                   /note="Helical; Name=4"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        197..209
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        210..230
FT                   /note="Helical; Name=5"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        231..257
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        258..278
FT                   /note="Helical; Name=6"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        279..286
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   REGION          1..35
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           115..117
FT                   /note="NPA 1"
FT   MOTIF           236..238
FT                   /note="NPA 2"
SQ   SEQUENCE   286 AA;  30753 MW;  397DECC763CD4638 CRC64;
     MAENKEEDVK LGANKFRETQ PLGTAAQTDK DYKEPPPAPL FEPGELSSWS FYRAGIAEFM
     ATFLFLYITI LTVMGLKRSD SLCSSVGIQG VAWAFGGMIF ALVYCTAGIS GGHINPAVTF
     GLFLARKLSL TRAVFYMVMQ CLGAICGAGV VKGFMVGPYQ RLGGGANVVN PGYTKGDGLG
     AEIIGTFVLV YTVFSATDAK RNARDSHVPI LAPLPIGFAV FLVHLATIPI TGTGINPARS
     LGAAIIYNDE HAWNDHWIFW VGPMIGAALA AIYHQIIIRA MPFHRS