PIP21_ORYSJ
ID PIP21_ORYSJ Reviewed; 290 AA.
AC Q8H5N9; A3BJC3; O65336; Q0D6R4;
DT 22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 25-MAY-2022, entry version 124.
DE RecName: Full=Probable aquaporin PIP2-1;
DE AltName: Full=OsPIP2;1;
DE AltName: Full=Plasma membrane intrinsic protein 2-1;
DE AltName: Full=Plasma membrane intrinsic protein 2a;
DE Short=PIP2a;
GN Name=PIP2-1; Synonyms=PIP2a;
GN OrderedLocusNames=Os07g0448800, LOC_Os07g26690;
GN ORFNames=OJ1047_A06.117, OsJ_023145;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND INDUCTION.
RC TISSUE=Meristem;
RX PubMed=10527423; DOI=10.1023/a:1006265528015;
RA Malz S., Sauter M.;
RT "Expression of two PIP genes in rapidly growing internodes of rice is not
RT primarily controlled by meristem activity or cell expansion.";
RL Plant Mol. Biol. 40:985-995(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [7]
RP NOMENCLATURE, TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=16033806; DOI=10.1093/pcp/pci172;
RA Sakurai J., Ishikawa F., Yamaguchi T., Uemura M., Maeshima M.;
RT "Identification of 33 rice aquaporin genes and analysis of their expression
RT and function.";
RL Plant Cell Physiol. 46:1568-1577(2005).
CC -!- FUNCTION: Aquaporins facilitate the transport of water and small
CC neutral solutes across cell membranes. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in roots, leaves and anthers.
CC {ECO:0000269|PubMed:10527423, ECO:0000269|PubMed:16033806}.
CC -!- INDUCTION: Down-regulated by chilling. {ECO:0000269|PubMed:10527423,
CC ECO:0000269|PubMed:16033806}.
CC -!- DOMAIN: Aquaporins contain two tandem repeats each containing three
CC membrane-spanning domains and a pore-forming loop with the signature
CC motif Asn-Pro-Ala (NPA).
CC -!- SIMILARITY: Belongs to the MIP/aquaporin (TC 1.A.8) family. PIP (TC
CC 1.A.8.11) subfamily. {ECO:0000305}.
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DR EMBL; AF062393; AAC16545.1; -; mRNA.
DR EMBL; AP003802; BAC15868.1; -; Genomic_DNA.
DR EMBL; AP008213; BAF21459.1; -; Genomic_DNA.
DR EMBL; AP014963; BAT01306.1; -; Genomic_DNA.
DR EMBL; CM000144; EAZ39662.1; -; Genomic_DNA.
DR EMBL; AK072519; -; NOT_ANNOTATED_CDS; mRNA.
DR PIR; T02879; T02879.
DR RefSeq; XP_015646828.1; XM_015791342.1.
DR AlphaFoldDB; Q8H5N9; -.
DR SMR; Q8H5N9; -.
DR STRING; 4530.OS07T0448800-01; -.
DR PaxDb; Q8H5N9; -.
DR PRIDE; Q8H5N9; -.
DR EnsemblPlants; Os07t0448800-01; Os07t0448800-01; Os07g0448800.
DR GeneID; 4343122; -.
DR Gramene; Os07t0448800-01; Os07t0448800-01; Os07g0448800.
DR KEGG; osa:4343122; -.
DR eggNOG; KOG0223; Eukaryota.
DR HOGENOM; CLU_020019_3_0_1; -.
DR InParanoid; Q8H5N9; -.
DR OMA; DKAWDHH; -.
DR OrthoDB; 1152704at2759; -.
DR Proteomes; UP000000763; Chromosome 7.
DR Proteomes; UP000007752; Chromosome 7.
DR Proteomes; UP000059680; Chromosome 7.
DR ExpressionAtlas; Q8H5N9; baseline and differential.
DR Genevisible; Q8H5N9; OS.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0015250; F:water channel activity; IBA:GO_Central.
DR CDD; cd00333; MIP; 1.
DR Gene3D; 1.20.1080.10; -; 1.
DR InterPro; IPR023271; Aquaporin-like.
DR InterPro; IPR034294; Aquaporin_transptr.
DR InterPro; IPR000425; MIP.
DR InterPro; IPR022357; MIP_CS.
DR PANTHER; PTHR45687; PTHR45687; 1.
DR Pfam; PF00230; MIP; 1.
DR PRINTS; PR00783; MINTRINSICP.
DR SUPFAM; SSF81338; SSF81338; 1.
DR TIGRFAMs; TIGR00861; MIP; 1.
DR PROSITE; PS00221; MIP; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Membrane; Reference proteome; Repeat; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..290
FT /note="Probable aquaporin PIP2-1"
FT /id="PRO_0000064034"
FT TRANSMEM 43..63
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 80..100
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TRANSMEM 131..151
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TRANSMEM 173..193
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TRANSMEM 207..227
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TRANSMEM 255..275
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT MOTIF 112..114
FT /note="NPA 1"
FT MOTIF 233..235
FT /note="NPA 2"
FT CONFLICT 11
FT /note="G -> C (in Ref. 5; EAZ39662)"
FT /evidence="ECO:0000305"
FT CONFLICT 89
FT /note="A -> S (in Ref. 1; AAC16545)"
FT /evidence="ECO:0000305"
FT CONFLICT 149
FT /note="K -> N (in Ref. 1; AAC16545)"
FT /evidence="ECO:0000305"
FT CONFLICT 152..153
FT /note="QS -> PN (in Ref. 1; AAC16545)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 290 AA; 30285 MW; 7C9ECC8F9968826D CRC64;
MGKDEVMESG GAAGEFAAKD YTDPPPAPLI DAAELGSWSL YRAVIAEFIA TLLFLYITVA
TVIGYKHQTD ASASGADAAC GGVGVLGIAW AFGGMIFILV YCTAGISGGH INPAVTFGLF
LARKVSLVRA ILYIVAQCLG AICGVGLVKA FQSAYFNRYG GGANTLAAGY SKGTGLAAEI
IGTFVLVYTV FSATDPKRNA RDSHVPVLAP LPIGFAVFMV HLATIPITGT GINPARSIGA
AVIFNNEKAW HNHWIFWVGP FVGAAIAAFY HQYILRAGAI KALGSFRSNA
