PIP25_MAIZE
ID PIP25_MAIZE Reviewed; 285 AA.
AC Q9XF58;
DT 01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 25-MAY-2022, entry version 123.
DE RecName: Full=Aquaporin PIP2-5;
DE AltName: Full=Plasma membrane intrinsic protein 2-5;
DE AltName: Full=ZmPIP2-5;
DE AltName: Full=ZmPIP2;5;
DE AltName: Full=ZmPIP2a;
GN Name=PIP2-5;
OS Zea mays (Maize).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea.
OX NCBI_TaxID=4577;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. B73; TISSUE=Root;
RX PubMed=11244102; DOI=10.1104/pp.125.3.1206;
RA Chaumont F., Barrieu F., Wojcik E., Chrispeels M.J., Jung R.;
RT "Aquaporins constitute a large and highly divergent protein family in
RT maize.";
RL Plant Physiol. 125:1206-1215(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], AND INDUCTION.
RC TISSUE=Root;
RX PubMed=14701934; DOI=10.1093/pcp/pcg168;
RA Lopez F., Bousser A., Sissoeff I., Gaspar M., Lachaise B., Hoarau J.,
RA Mahe A.;
RT "Diurnal regulation of water transport and aquaporin gene expression in
RT maize roots: contribution of PIP2 proteins.";
RL Plant Cell Physiol. 44:1384-1395(2003).
RN [3]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=10759498; DOI=10.1104/pp.122.4.1025;
RA Chaumont F., Barrieu F., Jung R., Chrispeels M.J.;
RT "Plasma membrane intrinsic proteins from maize cluster in two sequence
RT subgroups with differential aquaporin activity.";
RL Plant Physiol. 122:1025-1034(2000).
RN [4]
RP FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND
RP MUTAGENESIS OF GLY-104.
RX PubMed=14671024; DOI=10.1105/tpc.017194;
RA Fetter K., van Wilder V., Moshelion M., Chaumont F.;
RT "Interactions between plasma membrane aquaporins modulate their water
RT channel activity.";
RL Plant Cell 16:215-228(2004).
RN [5]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=16845476; DOI=10.1007/s11103-006-9022-1;
RA Hachez C., Moshelion M., Zelazny E., Cavez D., Chaumont F.;
RT "Localization and quantification of plasma membrane aquaporin expression in
RT maize primary root: a clue to understanding their role as cellular
RT plumbers.";
RL Plant Mol. Biol. 62:305-323(2006).
CC -!- FUNCTION: Water channel required to facilitate the transport of water
CC across cell membrane. Its function is impaired by Hg(2+). May play a
CC role in water uptake from the root surface. Active as homomers.
CC Increased activity when heteromerization with PIP1-2.
CC {ECO:0000269|PubMed:10759498, ECO:0000269|PubMed:14671024,
CC ECO:0000269|PubMed:16845476}.
CC -!- SUBUNIT: Homomers. May interact with PIP1-2 to form heteromers.
CC {ECO:0000269|PubMed:14671024}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:14671024};
CC Multi-pass membrane protein {ECO:0000269|PubMed:14671024}.
CC -!- TISSUE SPECIFICITY: Specifically expressed in roots, in the exodermis,
CC endodermis and xylem parenchyma. Polar localization to the external
CC periclinal side of epidermal cells in root apices.
CC {ECO:0000269|PubMed:10759498, ECO:0000269|PubMed:14671024,
CC ECO:0000269|PubMed:16845476}.
CC -!- INDUCTION: Expressed in roots with a circadian rhythm showing an
CC increase at the end of the night period, a peak during the first part
CC of the light period and then a decrease. {ECO:0000269|PubMed:14701934}.
CC -!- DOMAIN: Aquaporins contain two tandem repeats each containing three
CC membrane-spanning domains and a pore-forming loop with the signature
CC motif Asn-Pro-Ala (NPA).
CC -!- SIMILARITY: Belongs to the MIP/aquaporin (TC 1.A.8) family. PIP (TC
CC 1.A.8.11) subfamily. {ECO:0000305}.
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DR EMBL; AF130975; AAD28761.1; -; mRNA.
DR EMBL; AY243802; AAO86708.1; -; mRNA.
DR RefSeq; NP_001105616.1; NM_001112146.1.
DR AlphaFoldDB; Q9XF58; -.
DR SMR; Q9XF58; -.
DR STRING; 4577.GRMZM2G178693_P01; -.
DR TCDB; 1.A.8.11.7; the major intrinsic protein (mip) family.
DR PaxDb; Q9XF58; -.
DR ProMEX; Q9XF58; -.
DR EnsemblPlants; Zm00001eb077130_T001; Zm00001eb077130_P001; Zm00001eb077130.
DR GeneID; 542619; -.
DR Gramene; Zm00001eb077130_T001; Zm00001eb077130_P001; Zm00001eb077130.
DR KEGG; zma:542619; -.
DR eggNOG; KOG0223; Eukaryota.
DR HOGENOM; CLU_020019_3_0_1; -.
DR OMA; MMYMVAQ; -.
DR OrthoDB; 1152704at2759; -.
DR Proteomes; UP000007305; Chromosome 2.
DR ExpressionAtlas; Q9XF58; baseline and differential.
DR Genevisible; Q9XF58; ZM.
DR GO; GO:0016021; C:integral component of membrane; TAS:AgBase.
DR GO; GO:0005886; C:plasma membrane; IDA:AgBase.
DR GO; GO:0032991; C:protein-containing complex; TAS:AgBase.
DR GO; GO:0015250; F:water channel activity; IBA:GO_Central.
DR GO; GO:0051290; P:protein heterotetramerization; TAS:AgBase.
DR GO; GO:0051289; P:protein homotetramerization; TAS:AgBase.
DR CDD; cd00333; MIP; 1.
DR Gene3D; 1.20.1080.10; -; 1.
DR InterPro; IPR023271; Aquaporin-like.
DR InterPro; IPR034294; Aquaporin_transptr.
DR InterPro; IPR000425; MIP.
DR InterPro; IPR022357; MIP_CS.
DR PANTHER; PTHR45687; PTHR45687; 1.
DR Pfam; PF00230; MIP; 1.
DR PRINTS; PR00783; MINTRINSICP.
DR SUPFAM; SSF81338; SSF81338; 1.
DR TIGRFAMs; TIGR00861; MIP; 1.
DR PROSITE; PS00221; MIP; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Membrane; Reference proteome; Repeat; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..285
FT /note="Aquaporin PIP2-5"
FT /id="PRO_0000286022"
FT TRANSMEM 38..58
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 75..95
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TRANSMEM 126..146
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TRANSMEM 168..188
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TRANSMEM 202..222
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TRANSMEM 250..270
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT MOTIF 107..109
FT /note="NPA 1"
FT /evidence="ECO:0000250"
FT MOTIF 228..230
FT /note="NPA 2"
FT /evidence="ECO:0000250"
FT MUTAGEN 104
FT /note="G->W: Loss of water transport."
FT /evidence="ECO:0000269|PubMed:14671024"
SQ SEQUENCE 285 AA; 29836 MW; 871B8E5D9EA23F0C CRC64;
MAKDIEAAAA HEGKDYSDPP PAPLVDAEEL TKWSLYRAVI AEFVATLLFL YITVATVIGY
KHQTDAAASG PDAACGGVGV LGIAWAFGGM IFILVYCTAG VSGGHINPAV TFGLFLARKV
SLVRALLYIV AQCLGAICGV GLVKGFQSAF YVRYGGGANE LSAGYSKGTG LAAEIIGTFV
LVYTVFSATD PKRNARDSHV PVLAPLPIGF AVFMVHLATI PITGTGINPA RSLGAAVIYN
NDKAWDDHWI FWVGPFIGAA IAAAYHQYVL RASAAKLGSS ASFSR
