ID   PIPB2_SALTI             Reviewed;         350 AA.
AC   Q8Z4G9; Q7C7U8;
DT   20-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   25-MAY-2022, entry version 99.
DE   RecName: Full=Secreted effector protein PipB2;
DE   AltName: Full=Type III effector PipB2;
GN   Name=pipB2; OrderedLocusNames=STY2897, t2674;
OS   Salmonella typhi.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Salmonella.
OX   NCBI_TaxID=90370;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CT18;
RX   PubMed=11677608; DOI=10.1038/35101607;
RA   Parkhill J., Dougan G., James K.D., Thomson N.R., Pickard D., Wain J.,
RA   Churcher C.M., Mungall K.L., Bentley S.D., Holden M.T.G., Sebaihia M.,
RA   Baker S., Basham D., Brooks K., Chillingworth T., Connerton P., Cronin A.,
RA   Davis P., Davies R.M., Dowd L., White N., Farrar J., Feltwell T.,
RA   Hamlin N., Haque A., Hien T.T., Holroyd S., Jagels K., Krogh A.,
RA   Larsen T.S., Leather S., Moule S., O'Gaora P., Parry C., Quail M.A.,
RA   Rutherford K.M., Simmonds M., Skelton J., Stevens K., Whitehead S.,
RA   Barrell B.G.;
RT   "Complete genome sequence of a multiple drug resistant Salmonella enterica
RT   serovar Typhi CT18.";
RL   Nature 413:848-852(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700931 / Ty2;
RX   PubMed=12644504; DOI=10.1128/jb.185.7.2330-2337.2003;
RA   Deng W., Liou S.-R., Plunkett G. III, Mayhew G.F., Rose D.J., Burland V.,
RA   Kodoyianni V., Schwartz D.C., Blattner F.R.;
RT   "Comparative genomics of Salmonella enterica serovar Typhi strains Ty2 and
RT   CT18.";
RL   J. Bacteriol. 185:2330-2337(2003).
CC   -!- FUNCTION: Effector proteins function to alter host cell physiology and
CC       promote bacterial survival in host tissues. Involved in the
CC       reorganization of late endosome/lysosome (LE/Lys) compartments in
CC       mammalian cells. Necessary and sufficient to link kinesin-1 onto the
CC       Salmonella-containing vacuole (SCV) membrane. Required for centrifugal
CC       extension of lysosomal glycoprotein-rich membrane tubules, known as
CC       Salmonella-induced filaments (Sifs), away from the SCV and toward the
CC       cell periphery. Required for virulence, but not for intracellular
CC       survival and replication in phagocytic cells (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with the host kinesin light chain (KLC), a subunit
CC       of the kinesin-1 motor complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}. Host membrane
CC       {ECO:0000250}. Note=Secreted via the type III secretion system 2 (SPI-2
CC       TTSS), and delivered into the host cell. {ECO:0000250}.
CC   -!- DOMAIN: Contains various tandem pentapeptide repeats in the C-terminal
CC       region. The pentapeptide motif is required to efficiently recruit
CC       kinesin-1. No position is completely conserved in these repeats, whose
CC       consensus sequence is A-[DN]-[FLM]-X-X. The C-terminal 38 amino acid
CC       residues are required for peripheral localization of PipB2 and
CC       redistribution of lysosomal-associated membrane protein (LAMP). The N-
CC       terminal 225 amino acid residues are sufficient for type III
CC       translocation and association with Sifs and SCVs, but not accumulation
CC       in peripheral vesicles (By similarity). {ECO:0000250}.
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DR   EMBL; AL513382; CAD05888.1; -; Genomic_DNA.
DR   EMBL; AE014613; AAO70245.1; -; Genomic_DNA.
DR   RefSeq; NP_457179.1; NC_003198.1.
DR   RefSeq; WP_001801692.1; NZ_WSUR01000031.1.
DR   AlphaFoldDB; Q8Z4G9; -.
DR   SMR; Q8Z4G9; -.
DR   STRING; 220341.16503863; -.
DR   EnsemblBacteria; AAO70245; AAO70245; t2674.
DR   KEGG; stt:t2674; -.
DR   KEGG; sty:STY2897; -.
DR   PATRIC; fig|220341.7.peg.2948; -.
DR   eggNOG; COG1357; Bacteria.
DR   HOGENOM; CLU_067808_0_0_6; -.
DR   OMA; ADCDGAN; -.
DR   Proteomes; UP000000541; Chromosome.
DR   Proteomes; UP000002670; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0033644; C:host cell membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   InterPro; IPR001646; 5peptide_repeat.
DR   Pfam; PF00805; Pentapeptide; 4.
PE   3: Inferred from homology;
KW   Host membrane; Membrane; Repeat; Secreted; Virulence.
FT   CHAIN           1..350
FT                   /note="Secreted effector protein PipB2"
FT                   /id="PRO_0000278298"
FT   DOMAIN          162..201
FT                   /note="Pentapeptide repeat 1"
FT   DOMAIN          202..241
FT                   /note="Pentapeptide repeat 2"
FT   DOMAIN          247..286
FT                   /note="Pentapeptide repeat 3"
FT   DOMAIN          287..326
FT                   /note="Pentapeptide repeat 4"
SQ   SEQUENCE   350 AA;  36997 MW;  37E5428A0167955E CRC64;
     MQRSLDSLAG MATSAFGAGT SAAMRQATSP KTILQHIINF FTCGGIRRRN ETQYQELIET
     MAETLKSSMS DRGAPLPENI ILDDVDGCRV EFNLPGENNE AGQVIVRVSK GDNSETREIP
     LASFEKICRA LLFRCEFSLP QDSVILTAQG GMNLKGAVLT GANLTAENLC DADLSGADLE
     GAILFMADCD GANFKGANLS GASLGDSNLT NACLEDSIMC GATLDRANLT GANLQHTSLL
     GCSMVECNCS GANMDHANVS GSTLIRADMS GATLKGATIM AAIMEGAVLT RANLQKASFT
     ATNLDGADLS EANLRNTSFK DCTLTDLRTE DATMSTSTQT LFNVFYSENI