PIRA_DICDI
ID PIRA_DICDI Reviewed; 1336 AA.
AC Q6UK63; Q54JT5;
DT 29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=Protein pirA;
GN Name=pirA; Synonyms=pir121; ORFNames=DDB_G0287855;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND DISRUPTION PHENOTYPE.
RC STRAIN=AX3;
RX PubMed=12956949; DOI=10.1016/s0960-9822(03)00580-3;
RA Blagg S.L., Stewart M., Sambles C., Insall R.H.;
RT "PIR121 regulates pseudopod dynamics and SCAR activity in Dictyostelium.";
RL Curr. Biol. 13:1480-1487(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [3]
RP IDENTIFICATION IN THE WAVE COMPLEX.
RX PubMed=16581519; DOI=10.1016/j.cub.2006.02.068;
RA Ibarra N., Blagg S.L., Vazquez F., Insall R.H.;
RT "Nap1 regulates Dictyostelium cell motility and adhesion through SCAR-
RT dependent and -independent pathways.";
RL Curr. Biol. 16:717-722(2006).
CC -!- FUNCTION: Involved in regulation of actin and microtubule organization.
CC -!- SUBUNIT: Part of a Scar/WAVE complex containing brk1, scrA, abiA, pirA
CC and napA. {ECO:0000269|PubMed:16581519}.
CC -!- DISRUPTION PHENOTYPE: Cells have no developmental defects, but cells
CC are abnormally large and show a highly motile phenotype.
CC {ECO:0000269|PubMed:12956949}.
CC -!- SIMILARITY: Belongs to the CYFIP family. {ECO:0000305}.
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DR EMBL; AY368270; AAR89379.1; -; mRNA.
DR EMBL; AAFI02000104; EAL63574.1; -; Genomic_DNA.
DR RefSeq; XP_637067.1; XM_631975.1.
DR AlphaFoldDB; Q6UK63; -.
DR SMR; Q6UK63; -.
DR IntAct; Q6UK63; 2.
DR STRING; 44689.DDB0216270; -.
DR PaxDb; Q6UK63; -.
DR EnsemblProtists; EAL63574; EAL63574; DDB_G0287855.
DR GeneID; 8626322; -.
DR KEGG; ddi:DDB_G0287855; -.
DR dictyBase; DDB_G0287855; pirA.
DR eggNOG; KOG3534; Eukaryota.
DR HOGENOM; CLU_002688_2_1_1; -.
DR InParanoid; Q6UK63; -.
DR OMA; MPKVCKL; -.
DR PhylomeDB; Q6UK63; -.
DR Reactome; R-DDI-2029482; Regulation of actin dynamics for phagocytic cup formation.
DR Reactome; R-DDI-5663213; RHO GTPases Activate WASPs and WAVEs.
DR Reactome; R-DDI-6798695; Neutrophil degranulation.
DR Reactome; R-DDI-9013149; RAC1 GTPase cycle.
DR Reactome; R-DDI-9013404; RAC2 GTPase cycle.
DR Reactome; R-DDI-9013423; RAC3 GTPase cycle.
DR PRO; PR:Q6UK63; -.
DR Proteomes; UP000002195; Chromosome 5.
DR GO; GO:0031252; C:cell leading edge; IC:dictyBase.
DR GO; GO:0005911; C:cell-cell junction; IC:dictyBase.
DR GO; GO:0032433; C:filopodium tip; IC:dictyBase.
DR GO; GO:0031143; C:pseudopodium; IC:dictyBase.
DR GO; GO:0031209; C:SCAR complex; IDA:dictyBase.
DR GO; GO:0000340; F:RNA 7-methylguanosine cap binding; IBA:GO_Central.
DR GO; GO:0031267; F:small GTPase binding; IMP:dictyBase.
DR GO; GO:0000902; P:cell morphogenesis; IMP:dictyBase.
DR GO; GO:0048870; P:cell motility; IMP:dictyBase.
DR GO; GO:0046847; P:filopodium assembly; IMP:dictyBase.
DR GO; GO:0000281; P:mitotic cytokinesis; IMP:dictyBase.
DR GO; GO:0030835; P:negative regulation of actin filament depolymerization; IMP:dictyBase.
DR GO; GO:0031269; P:pseudopodium assembly; IMP:dictyBase.
DR GO; GO:0030833; P:regulation of actin filament polymerization; IEA:InterPro.
DR GO; GO:0006417; P:regulation of translation; IBA:GO_Central.
DR InterPro; IPR009828; CYRIA/CYRIB_Rac1-bd.
DR InterPro; IPR008081; Cytoplasmic_FMR1-int.
DR PANTHER; PTHR12195; PTHR12195; 1.
DR Pfam; PF07159; DUF1394; 1.
DR Pfam; PF05994; FragX_IP; 1.
DR PIRSF; PIRSF008153; FMR1_interacting; 1.
DR PRINTS; PR01698; CYTOFMRPINTP.
PE 1: Evidence at protein level;
KW Reference proteome.
FT CHAIN 1..1336
FT /note="Protein pirA"
FT /id="PRO_0000331388"
FT REGION 1262..1336
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1262..1286
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1288..1310
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1319..1336
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1336 AA; 153205 MW; 381D32E0D2412A8E CRC64;
MSVDPKQSKI ADVFERCDVL ESFPFHDDQS EIEEQSPSIG YDAYDKSLNY TDRGAYETQW
SEETIGMEKM EEVLKQGDSF INMVYTYRSC SKALPTVKTA EQVNKTQIYE GNFEVLEPEI
KKLKDFMYFQ KDTIKLFCDH IKKLASTYDK KKETISASES FINYLVRILD LLAILDALKN
MKACLNNDFS FFKRATGFLR KQMSGTEDQT QENHTLYLFL ANQNSITSSL KLELHNIDKF
DDILPMIVNQ CADYLEQEKY ILPSEKHCLL RVMPFVLFLI DENDSKHNIN KNKNLNISRY
AKIFKKNPVV PLYGDMQITL ESLVKRSPHF DEKAWGTSTL DSKSALDYEI IHVLDQTRSL
YNEYMARFAN MVNEIRAAKA RGPKEPLPLA ESDIQAITLM GLRILSDWTS RVLQQSAWKY
SKPNNDPTIS ATFDYERVVK FNYTKEERTA LVQLIAMIKS LASLMMKSET LLQPILRKTI
HQELQEFVQI NLKETIKSFV KNNPKKKDNI KLEMSQLKNI SVDWFSGFEP AEAVPNKKSK
EVEEKVQIPA RAVPPSPTQL ELILTLVSSL MDKKKDFSSD QYRDFEAFSS KAFFYRYLLS
LSSSIISITD LADLWYREFY LELNNRVQFP IETSLPWILT DHILESDDPS LIEHLFYPLG
LYNDTAQRAL LSLNQRFLYD EIEAELNLCF DQLLYKLSGK VYTHFKTQAS SILLDKPYKT
QLELAHFNGK LHTPKSRFDV LLRQKHITLL GRSIDLCGLL AQRQNNTIRQ NLDYAISRFE
ACDLTSIVEL ETQIANIKLT HKLLSEYFDI DPFESIFNEI NESTSLVSYH GRIVLHIIFE
LVADFAPNYT FNSITQRFIK APYTFTEELK RDALPKTNPV FLFGNKYLNA AYANSIELYK
NFIGVPHIQS ILRIVGKKNL PLIVAEVLRN IEIKITNVLS PYVSELLQGM PASTKLPIYD
YGTEGGYGYF QLKLKDIYIY PDLRPEVLQT FRELGNSLVF MNLLDQVITQ TDSYNFAKAA
PFLGITPETW KDLEPSTEDP TLQSPLYSQL QKLAQLLESR PEVAKSSQSL NDMVANAWRA
DKFYRPSEQN FSLFKSVLQR FSSILNIVRH DWSGAPPDNG VICVDSSTEF YRLWSALQFV
ICWPLSNEND KSFHELFGDG LMWAGCSLIH FLGQQYRFEL FDFCYHILNV EEAAAVRSDK
PALKNFFKNA QIIKDINNQI FSILNTYCPP PPPSNMILSP PATEQAEQFI VTTTVEEQSI
QQQQQQQQQQ QLQQSTSNSG SSLSLSAAGA PPPPPPPSTN GLPPPPPMAE YEMSSSIDDF
APPPPPPFGM PPPPPM
