ID   PIRC2_BOVIN             Reviewed;         120 AA.
AC   A0A3Q1LFK7;
DT   25-MAY-2022, integrated into UniProtKB/Swiss-Prot.
DT   10-APR-2019, sequence version 1.
DT   03-AUG-2022, entry version 10.
DE   RecName: Full=Piercer of microtubule wall 2 protein;
GN   Name=PIERCE2;
GN   Synonyms=C10H15orf65 {ECO:0000312|VGNC:VGNC:108125}, C15orf65;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913 {ECO:0000312|Proteomes:UP000009136};
RN   [1] {ECO:0000312|Proteomes:UP000009136}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Hereford {ECO:0000312|Proteomes:UP000009136};
RA   Rosen B.D., Bickhart D.M., Koren S., Schnabel R.D., Hall R., Zimin A.,
RA   Dreischer C., Schultheiss S., Schroeder S.G., Elsik C.G., Couldrey C.,
RA   Liu G.E., Van Tassell C.P., Phillippy A.M., Smith T.P.L., Medrano J.F.;
RT   "ARS-UCD1.2.";
RL   Submitted (MAR-2018) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   IDENTIFICATION.
RC   STRAIN=Hereford;
RG   Ensembl;
RL   Submitted (JAN-2019) to UniProtKB.
RN   [3] {ECO:0007744|PDB:7RRO}
RP   STRUCTURE BY ELECTRON MICROSCOPY (3.40 ANGSTROMS), TISSUE SPECIFICITY,
RP   SUBCELLULAR LOCATION, INTERACTION WITH CFAP53; ODAD1 AND ODAD3, AND
RP   FUNCTION.
RX   PubMed=34715025; DOI=10.1016/j.cell.2021.10.007;
RA   Gui M., Farley H., Anujan P., Anderson J.R., Maxwell D.W., Whitchurch J.B.,
RA   Botsch J.J., Qiu T., Meleppattu S., Singh S.K., Zhang Q., Thompson J.,
RA   Lucas J.S., Bingle C.D., Norris D.P., Roy S., Brown A.;
RT   "De novo identification of mammalian ciliary motility proteins using cryo-
RT   EM.";
RL   Cell 184:5791-5806.e19(2021).
CC   -!- FUNCTION: Microtubule inner protein involved in the attachment of outer
CC       dynein arms (ODAs) to dynein-decorated doublet microtubules (DMTs) in
CC       cilia axoneme, which is required for motile cilia beating.
CC       {ECO:0000305|PubMed:34715025}.
CC   -!- SUBUNIT: Interacts with CFAP53, ODAD1 and ODAD3; the interactions link
CC       the outer dynein arms docking complex (ODA-DC) to the internal
CC       microtubule inner proteins (MIP) in cilium axoneme.
CC       {ECO:0000269|PubMed:34715025}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, cilium axoneme
CC       {ECO:0000269|PubMed:34715025}.
CC   -!- TISSUE SPECIFICITY: Expressed in trachea multiciliated cells.
CC       {ECO:0000269|PubMed:34715025}.
CC   -!- SIMILARITY: Belongs to the PIERCE2 family. {ECO:0000305}.
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DR   PDB; 7RRO; EM; 3.40 A; G=1-120.
DR   PDBsum; 7RRO; -.
DR   SMR; A0A3Q1LFK7; -.
DR   Ensembl; ENSBTAT00000084534; ENSBTAP00000056977; ENSBTAG00000051675.
DR   VEuPathDB; HostDB:ENSBTAG00000051675; -.
DR   VGNC; VGNC:108125; PIERCE2.
DR   GeneTree; ENSGT00940000154745; -.
DR   OMA; MIMYKTS; -.
DR   Proteomes; UP000009136; Chromosome 10.
DR   Bgee; ENSBTAG00000051675; Expressed in spermatid and 104 other tissues.
DR   GO; GO:0005879; C:axonemal microtubule; IDA:UniProtKB.
DR   GO; GO:0035082; P:axoneme assembly; ISS:UniProtKB.
DR   GO; GO:0003341; P:cilium movement; ISS:UniProtKB.
DR   GO; GO:0007368; P:determination of left/right symmetry; ISS:UniProtKB.
DR   InterPro; IPR026507; UPF0691.
DR   PANTHER; PTHR20899; PTHR20899; 1.
DR   Pfam; PF14892; DUF4490; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cell projection; Cytoplasm; Cytoskeleton; Reference proteome.
FT   CHAIN           1..120
FT                   /note="Piercer of microtubule wall 2 protein"
FT                   /id="PRO_0000455516"
FT   REGION          1..25
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   120 AA;  13771 MW;  841FB8D726166EA7 CRC64;
     MTECDWEKKS TSASNSDTEM KPELPPCVNP GNPVFSCMLD PKTLHTTTSL SKPKMIMYKT
     NSSNYGEFLP MPQFFPCYYT PREQVFSSHI RATGFYQNNT LNTAPDRTRT LDFPNFQHTL